Core2: Biophysics and Metabolic Imaging Core

核心2：生物物理学和代谢成像核心

• 批准号: 10020778
• 负责人: WARREN R ZIPFEL
• 金额: $ 13.13万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10020778
• 关键词: Anisotropy; Architecture; Area; Award; Back; Biological; Biomechanics; Biomedical Engineering; Biophysics; Biotechnology; Cell membrane; Cells; Cellular Mechanotransduction; Chemicals; Code; Collaborations; Computer Hardware; Custom; Data; Data Analyses; Development; Engineering; Environment; Equipment; Extracellular Matrix; Faculty; Fluorescence; Fluorescence Resonance Energy Transfer; Functional Imaging; Future; Glycocalyx; Goals; Grant; Image; Image Analysis; Imaging Device; Infrastructure; Label; Lead; Lighting; Link; Measurement; Metabolic; Metabolism; Methods; Microfabrication; Microscope; Microscopy; Minor; Modeling; Modification; Molecular Analysis; NADH; Optics; Patients; Physics; Play; Protocols documentation; Research; Research Personnel; Resolution; Resources; Role; Sampling; Services; Software Tools; Spectrum Analysis; Standardization; Structure; Substrate Interaction; System; Technology; Testing; Time; Tissues; Validation; Visualization; Work; base; biophysical analysis; biophysical model; cancer cell; cellular imaging; design; fluorescence imaging; image processing; imaging platform; imaging system; improved; innovation; instrument; instrumentation; light microscopy; member; metabolic imaging; microscopic imaging; multiphoton imaging; nanoscale; new technology; novel; optical imaging; phosphorescence; programs; second harmonic generation imaging; sensor; stem cells; success; technology development; tumor metabolism; two photon microscopy; two-photon

Project Summary - Biophysics and Metabolic Imaging Core The Biophysics and Metabolic Imaging Core will provide the instrumentation, facilities and expertise required to meet the optical microscopy and imaging analysis needs of the three Projects detailed in this application, as well as those of any PSOC pilot or trans-network projects that develop as a result of this Center program. In particular, the Core will provide the imaging infrastructure and expertise required for imaging of the cellular metabolic state, tissue structural features, and the general visualization of the biological samples tested in this Center. The Biophysics and Metabolic Imaging Core is made up of four overall aims: Aim 1: We will provide instrumentation and analysis support for high resolution quantitative optical imaging-based measurements of cellular metabolic state using new genetically encoded metabolite-sensing indicators and label-free two-photon excited NADH/FAD ratio imaging. Aim 2: We will develop a set of novel hardware platforms that will introduce further improved methods for carrying out FRET-based metabolic sensor imaging and combined lifetime/polarization anisotropy NADH imaging. We will also enable high throughput, high content, and long time-course imaging of metabolism and cellular features on the single cell level in culture systems and tissue explants such as patient-derived xenographs (PDXs). Aim 3: We will construct and utilize a new type of super-resolution interference-based microscope with 10 nm axial resolution that is especially designed to probe the cell membrane/ glycocalyx – substrate interactions that play a critical role in how cancer cells interact with their environment. Aim 4: We will provide the computational expertise and computer hardware for the image processing and analysis required to evaluate the image data acquired in Aims 1-3. The Biophysics and Metabolic Imaging Core is co-directed by two recognized experts in biological imaging and provides cutting-edge instrumentation and expertise that will be critical to the success the overall project. The Biophysics and Metabolic Imaging Core will closely interact with the three Projects and with the Tissue Microfabrication Core on applications of our existing technologies and the development of new technologies for task-specific metabolic and functional imaging, image-based biophysical modeling, and analytical image analysis.

项目摘要 - 生物物理学和代谢成像核心 生物物理学和代谢成像核心将提供仪器、设施和专业知识 需要满足本节详细介绍的三个项目的光学显微镜和成像分析需求 应用程序，以及作为该中心开发的任何 PSOC 试点或跨网络项目的应用程序 特别是，核心将提供成像所需的成像基础设施和专业知识。 细胞代谢状态、组织结构特征和生物样品的总体可视化 生物物理学和代谢成像核心由四个总体目标组成：目标 1： 我们将为基于高分辨率定量光学成像的仪器和分析提供支持 使用新的基因编码代谢物传感指标测量细胞代谢状态 无标记双光子激发 NADH/FAD 比率成像。目标 2：我们将开发一套新颖的硬件。 平台将进一步引入改进的方法来进行基于 FRET 的代谢传感器成像 以及组合寿命/偏振各向异性 NADH 成像，我们还将实现高通量、高通量。 培养物中单细胞水平的代谢和细胞特征的内容和长时程成像 目标 3：我们将构建和利用患者来源的异种移植系统和组织外植体。 一种新型超分辨率干涉显微镜，具有 10 nm 轴向分辨率，特别是 旨在探测细胞膜/糖萼-底物相互作用，这些相互作用在癌症发生过程中发挥着关键作用 目标 4：我们将提供计算专业知识和计算机。 用于评估目标 1-3 中获取的图像数据所需的图像处理和分析的硬件。 生物物理学和代谢成像核心由两位生物成像和代谢领域公认的专家共同指导 提供对整个项目成功至关重要的尖端仪器和专业知识。 生物物理学和代谢成像核心将与这三个项目以及组织密切互动 微细加工核心：现有技术的应用和新技术的开发 特定任务的代谢和功能成像、基于图像的生物物理建模和分析图像 分析。