Core2: Biophysics and Metabolic Imaging Core

核心2：生物物理学和代谢成像核心

• 批准号: 10020778
• 负责人: WARREN R ZIPFEL
• 金额: $ 13.13万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10020778
• 关键词: Anisotropy; Architecture; Area; Award; Back; Biological; Biomechanics; Biomedical Engineering; Biophysics; Biotechnology; Cell membrane; Cells; Cellular Mechanotransduction; Chemicals; Code; Collaborations; Computer Hardware; Custom; Data; Data Analyses; Development; Engineering; Environment; Equipment; Extracellular Matrix; Faculty; Fluorescence; Fluorescence Resonance Energy Transfer; Functional Imaging; Future; Glycocalyx; Goals; Grant; Image; Image Analysis; Imaging Device; Infrastructure; Label; Lead; Lighting; Link; Measurement; Metabolic; Metabolism; Methods; Microfabrication; Microscope; Microscopy; Minor; Modeling; Modification; Molecular Analysis; NADH; Optics; Patients; Physics; Play; Protocols documentation; Research; Research Personnel; Resolution; Resources; Role; Sampling; Services; Software Tools; Spectrum Analysis; Standardization; Structure; Substrate Interaction; System; Technology; Testing; Time; Tissues; Validation; Visualization; Work; base; biophysical analysis; biophysical model; cancer cell; cellular imaging; design; fluorescence imaging; image processing; imaging platform; imaging system; improved; innovation; instrument; instrumentation; light microscopy; member; metabolic imaging; microscopic imaging; multiphoton imaging; nanoscale; new technology; novel; optical imaging; phosphorescence; programs; second harmonic generation imaging; sensor; stem cells; success; technology development; tumor metabolism; two photon microscopy; two-photon

Project Summary - Biophysics and Metabolic Imaging Core The Biophysics and Metabolic Imaging Core will provide the instrumentation, facilities and expertise required to meet the optical microscopy and imaging analysis needs of the three Projects detailed in this application, as well as those of any PSOC pilot or trans-network projects that develop as a result of this Center program. In particular, the Core will provide the imaging infrastructure and expertise required for imaging of the cellular metabolic state, tissue structural features, and the general visualization of the biological samples tested in this Center. The Biophysics and Metabolic Imaging Core is made up of four overall aims: Aim 1: We will provide instrumentation and analysis support for high resolution quantitative optical imaging-based measurements of cellular metabolic state using new genetically encoded metabolite-sensing indicators and label-free two-photon excited NADH/FAD ratio imaging. Aim 2: We will develop a set of novel hardware platforms that will introduce further improved methods for carrying out FRET-based metabolic sensor imaging and combined lifetime/polarization anisotropy NADH imaging. We will also enable high throughput, high content, and long time-course imaging of metabolism and cellular features on the single cell level in culture systems and tissue explants such as patient-derived xenographs (PDXs). Aim 3: We will construct and utilize a new type of super-resolution interference-based microscope with 10 nm axial resolution that is especially designed to probe the cell membrane/ glycocalyx – substrate interactions that play a critical role in how cancer cells interact with their environment. Aim 4: We will provide the computational expertise and computer hardware for the image processing and analysis required to evaluate the image data acquired in Aims 1-3. The Biophysics and Metabolic Imaging Core is co-directed by two recognized experts in biological imaging and provides cutting-edge instrumentation and expertise that will be critical to the success the overall project. The Biophysics and Metabolic Imaging Core will closely interact with the three Projects and with the Tissue Microfabrication Core on applications of our existing technologies and the development of new technologies for task-specific metabolic and functional imaging, image-based biophysical modeling, and analytical image analysis.

项目摘要 - 生物物理学和代谢成像核心 生物物理学和代谢成像核心将提供仪器，设施和专业知识 满足这三个项目的光学显微镜和成像分析需求的需求 以及该中心产生的任何PSOC飞行员或跨网络项目的应用程序 程序。特别是，核心将提供成像的成像基础架构和专业知识 细胞代谢状态，组织结构特征和生物样品的一般可视化 在这个中心进行了测试。生物物理学和代谢成像核心由四个总体目标组成：目标1： 我们将为高分辨率定量光学成像提供仪器和分析支持 使用新的一般编码的代谢物感应指标和 无标签的双光子激发NADH/FAD比率成像。目标2：我们将开发一组新颖的硬件 平台将引入进一步的改进方法，用于执行基于FRET的代谢传感器成像 并结合寿命/极化各向异性NADH成像。我们还将启用高吞吐量 含量，以及培养物中新陈代谢和细胞特征的长时间成像 系统和组织外植体，例如患者衍生的Xenographs（PDXS）。目标3：我们将构建和利用 一种具有10 nm轴向分辨率的新型超分辨率干扰显微镜，尤其是 旨在探测细胞膜/糖卵形 - 底物相互作用，这些相互作用在癌症中起着至关重要的作用 细胞与他们的环境相互作用。目标4：我们将提供计算专业知识和计算机 用于评估目标1-3中获得的图像数据所需的图像处理和分析的硬件。这 生物物理学和代谢成像核心由两位公认的生物成像专家和 提供尖端的仪器和专业知识，这对于整个项目的成功至关重要。 生物物理学和代谢成像核心将与三个项目和组织紧密相互作用 微加工核心在我们现有技术的应用以及开发新技术的应用 特定于任务的代谢和功能成像，基于图像的生物物理建模和分析图像 分析。