UNCOVERING HIDDEN IN VIVO RESONANCES USING EDITING BASED

使用基于编辑的方法揭示隐藏的体内共振

• 批准号: 7601632
• 负责人: MALGORZATA MARJANSKA
• 金额: $ 2.36万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601632
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Coupling; Funding; Glucose; Grant; Institution; Lasers; Lipids; Methods; Physiologic pulse; Protons; Pulse taking; Research; Research Personnel; Resources; Signal Transduction; Source; System; Techniques; United States National Institutes of Health; Water; base; in vivo; methyl lactate; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A novel single-shot spectral editing technique for in vivo proton NMR is proposed to recover resonances of low-concentration metabolites obscured by very strong resonances. With this new method, editing is performed by transferring transverse magnetization to J-coupled spins from selected coupling partners using a homonuclear Hartmann-Hahn polarization transfer with adiabatic pulses. The current implementation uses 1D-TOCSY with single-voxel localization based on LASER to recover the H1 proton of beta-glucose at 4.63 ppm from under water and the lactate methyl resonances from beneath a strong lipid signal. The method can be extended to further spin systems where conventional editing methods are difficult to perform.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 提出了一种用于体内质子 NMR 的新型单次光谱编辑技术，以恢复被非常强的共振掩盖的低浓度代谢物的共振。通过这种新方法，通过使用绝热脉冲的同核 Hartmann-Hahn 极化转移将横向磁化从选定的耦合伙伴转移到 J 耦合自旋来进行编辑。当前的实施使用基于激光的单体素定位的 1D-TOCSY，从水下恢复 4.63 ppm 的 β-葡萄糖的 H1 质子，并从强脂质信号下恢复乳酸甲基共振。该方法可以扩展到传统编辑方法难以执行的其他旋转系统。