Understanding and using microbial conductive nanowires

了解和使用微生物导电纳米线

• 批准号: 10705196
• 负责人: Fengbin Wang
• 金额: $ 24.9万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10705196
• 关键词: Address; Algorithms; Amaze; Antibodies; Archaea; Bacteria; Biochemical; Biocompatible Materials; Bioinformatics; Biophysical Process; Biosensor; Cells; Collaborations; Cryoelectron Microscopy; Cytochrome c Group; Cytochromes; Data; Electron Transport; Electronics; Electrons; Fiber; Filament; Fimbriae Proteins; Future; Genetic; Geobacter; Growth; Heme; Hydrogen; Knowledge; Label; Lead; Measurement; Measures; Medical; Mentors; Messenger RNA; Microbe; Microscopic; Modeling; Molecular; Morphology; Organism; Oxidants; Oxidation-Reduction; Peptides; Pilum; Point Mutation; Polymers; Prokaryotic Cells; Proteins; Proteomics; Pyrobaculum; Recombinants; Resolution; Respiration; Role; Scaffolding Protein; Source; Structure; Surface; Techniques; Validation; Work; appendage; biophysical properties; biophysical techniques; design; extracellular; heme C; imaging approach; insight; interest; microbial; monomer; mutant; nanofilament; nanowire; novel; rational design; scaffold; self assembly; synthetic biology

Abstract Long-range (>10 μm) transport of electrons along networks of G. sulfurreducens protein filaments, known as microbial nanowires, has been invoked to explain a wide range of globally important redox phenomena. The remarkable electronic conduction capability of those nanowires has sparked a great deal of interest in the medical application space, such as for building biocompatible materials and biosensor. For more than a decade, G. sulfurreducens nanowires were thought to be bacterial type IV pili, supported by many indirect genetic and biochemical observations. Recently we showed that these conductive nanowires are not made of type IV pilins. Instead, these structures are a polymerized multi-heme c-type cytochrome, OmcS, which have never been characterized before. The OmcS filament model is consistent with the known roles of OmcS in Geobacter respiration, but our knowledge of cytochrome appendages is still very limited. This study aims at addressing fundamental scientific questions about cytochrome filaments in respiring prokaryotes as well as applying our discoveries into the general medical field. Specifically, I will: A) identify and characterize novel cytochrome filaments in bacterial and archaeal strains, through bioinformatics algorithms followed by microscopic validation. B) Then I will study the conduction mechanism of these filaments by high resolution cryogenic electron microscopy (cryo-EM) and conductivity measurement. C) Finally, based on these new insights into cytochrome filaments, I will create a novel design for a self-assembled conductive nanowire. These nanowires may be derived directly from a novel cytochrome filament or may contain a peptide/protein based self-assembled scaffold core with soluble cytochromes appended to the outer surface. The results will advance our understanding of cytochrome nanowires, as well as generating self-assembling nanowire scaffolds that may be used in many future biomedical applications.

抽象的 电子沿G.硫杆菌蛋白丝的网络的远距离运输（>10μm） 已调用微生物纳米线来解释广泛的全球重要氧化还原现象。 这些纳米线的显着电子传导能力引起了医疗的极大兴趣 应用空间，例如构建生物相容性材料和生物传感器。十多年来，G。 硫糖纳米线被认为是IV型细菌，由许多间接遗传和 生化观察。最近，我们表明这些导电纳米线不是由IV型PILIN制成的。 取而代之的是，这些结构是聚合的多血红素C型细胞色素，OMC，从未有过 以前的特征。 OMC丝模型与OMC在Geobacter中的已知作用一致 呼吸，但是我们对细胞色素附属物的了解仍然非常有限。这项研究旨在解决 关于呼吸原核生物的细胞色素细丝的基本科学问题以及应用我们的 发现通用医疗领域。具体来说，我将：a）识别和表征新颖的细胞色素 细菌和古细菌菌株的细丝，通过生物信息学算法，然后是微观验证。 b）然后我将通过高分辨率低温电子研究这些细丝的传导机制 显微镜（冷冻EM）和电导率测量。 c）最后，基于对细胞色素的这些新见解 细丝，我将为自组装的导电纳米线创建一种新颖的设计。这些纳米线可能是 直接源自新型的细胞色素丝或可能包含基于肽/蛋白质的自组装支架 带有固体细胞色素的核心附加到外表面。结果将提高我们对 细胞色素纳米线，并产生自组装的纳米线脚手架 未来的生物医学应用。