Developing an Electrochemical Assay to Detect Nerve Regeneration in 3D Scaffolds

开发电化学检测方法来检测 3D 支架中的神经再生

基本信息

批准号：
8603858
负责人：
AMY B HARKINS
金额：
$ 7.28万
依托单位：
SAINT LOUIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-01-15 至 2014-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8603858
关键词：
3-Dimensional Alginates Amyotrophic Lateral Sclerosis Analytical Chemistry Animal Model Animals Applications Grants Atrophic Biocompatible Materials Biological Assay Biology Cell Survival Charge Chitosan Collagen Communication Detection Development Devices Diabetic Neuropathies Dimensions Disease Engineering Eye Failure Film Future Goals Growth Health Human In Vitro Individual Laminin Length Measurement Measures Medical Medical Research Methods Modeling Modification Muscle Muscle Contraction Nafion Natural regeneration Nerve Nerve Regeneration Neural Conduction Neurites Neurons Organ Outcome PSSO3 Phase Process Research Retinal Degeneration Sepharose Signal Transduction Solutions Staging Surface Synapses Techniques Testing Time Tissue Engineering Tissues Trauma Work World Health base carbon fiber clinically relevant design detector human tissue improved in vivo insight meetings nerve supply neuron loss public health relevance regenerative reinnervation repaired response restoration scaffold two-dimensional

项目摘要

DESCRIPTION (provided by applicant): The research objective of this proposal is to develop a method to detect whether regenerating neurons are communicating appropriately before they regrow to their target tissues. Currently, successful regeneration of nerves in vivo is evaluated by nerve conduction velocity and muscle contraction. The major drawback to currently available methods is that either complete reconnectivity or final reinnervation of target tissues must occur for testing to be possible. The proposed technique will utilize a multi-disciplinary approach to detect synaptic signals as a measure of proper nerve growth in the initial stages of regeneration, rather than waiting until the nerve has reconnected and innervated target tissues. The aims to accomplish this objective are (i) to characterize synaptic signals from neurons regrowing on a 2 dimensional surface, or within a 3 dimensional scaffold, and detected with an x-y multi-channel interdigitated array detector, and (ii) to design a 3 dimensional (x-y-z) interdigitated array detector to measure synaptic signals from regrowing neurons within clinically relevant 3D scaffold materials. The long-term outcome of this project has the potential to meet the global need for a practical and inexpensive method to rapidly assess the efficacy of nerve regeneration.

描述（由申请人提供）：本提案的研究目标是开发一种方法来检测再生神经元在重新生长到目标组织之前是否进行适当的通信。目前，体内神经再生的成功是通过神经传导速度和肌肉收缩来评估的。目前可用方法的主要缺点是，必须进行目标组织的完全重新连接或最终神经支配才能进行测试。所提出的技术将利用多学科方法来检测突触信号，作为再生初始阶段神经正常生长的衡量标准，而不是等到神经重新连接并支配目标组织。实现这一目标的目标是 (i) 表征在 2 维表面或 3 维支架内重新生长的神经元的突触信号，并使用 x-y 多通道叉指阵列检测器进行检测，以及 (ii) 设计一个 3三维 (x-y-z) 叉指阵列探测器，用于测量临床相关 3D 支架材料内再生神经元的突触信号。该项目的长期成果有可能满足全球对一种实用且廉价的方法来快速评估神经再生功效的需求。