CORE C- CELL AND TISSUE CULTURE CORE

核心 C 细胞和组织培养核心

• 批准号: 7683524
• 负责人: CAROLYN L SMITH
• 金额: $ 13.28万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7683524
• 关键词: Adenoviruses; American Type Culture Collection; Animal Experiments; Animals; Antibiotics; Antisense Oligonucleotides; Arts; Azacitidine; Biopsy; Buffers; Cattle; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Charcoal; Culture Media; Cyclic AMP; Decidual Cell Reactions; Deoxyribonuclease I; Detection; Dextrans; Dialysis procedure; Embryo; Endometrial Stromal Cell; Endometrium; Enzymes; Eukaryotic Cell; Fertilization in Vitro; Fibroblasts; Forskolin; Freezing; Gentamicins; Growth Factor; Hela Cells; Hormones; Human; In Vitro; Incubated; Individual; Insulin; Laboratories; MCF7 cell; Maintenance; Mammalian Cell; Microscopy; Modeling; Mus; Mycoplasma; Ovarian Granulosa Cell; Ovum; PC12 Cells; Penicillins; Primary Cell Cultures; Procedures; Production; Quality Control; Reagent; Reproducibility; Research Personnel; Serum; Small Interfering RNA; Sodium Chloride; Sodium Selenite; Solutions; Staining method; Stains; Steroids; Streptomycin; Structure of thyroid parafollicular cell; Subfamily lentivirinae; Technology; Testing; Time; Tissue Sample; Tissues; Training; Transferrin; Transgenic Mice; Trichostatin A; United States National Institutes of Health; Vitamins; Work; biological research; collagenase; cost; design; dextran; embryo culture; embryonic stem cell; established cell line; granulosa cell; improved; oocyte maturation; reproductive; research study; salt balance; small hairpin RNA; steroid hormone; tissue culture; tissue/cell culture

The Cell and Tissue Culture Core laboratory is designed to provide state-of-the-art tissue and cell culture facilities to the individual projects of the Center for Reproductive Biological Research. This Core will perform in vitro culture of immortilized and primary mammalian cells, murine embryonic stem cell manipulations and lentivirus and adenovirus production, as required by Center investigators and their staff. In addition, this facility will train Core users in the use of in vitro cell culture technologies. A combination of a common, well-equipped tissue culture facility and centralized purchasing of standard and specialized cell culture reagents directly benefits individual projects by improving reproducibility, time utilization and cost management.

细胞和组织培养核心实验室旨在提供最新的组织和细胞培养 生殖生物学研究中心各个项目的设施。这个核心将在 永生和原发性哺乳动物细胞，鼠类胚胎干细胞操作的体外培养 慢病毒和腺病毒生产，按中心调查人员及其工作人员的要求。此外，这个设施 将培训核心用户使用体外细胞培养技术。共同，设备齐全的结合 直接对标准和专业细胞培养试剂进行组织培养设施以及集中购买 通过改善可重复性，时间利用率和成本管理来使单个项目受益。