Supplement: CRISPR screens of population relevant genes governing toxicant resilience

补充：CRISPR 筛选控制毒物抵抗力的群体相关基因

基本信息

批准号：
10720972
负责人：
CHRISTOPHER D VULPE
金额：
$ 36.64万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-02-14 至 2026-11-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10720972
关键词：
Afferent Neurons Alleles Ataxia CRISPR library CRISPR screen Candidate Disease Gene Cardiac Myocytes Categories Cell Line Cell model Cells Characteristics Chromosome Mapping Clustered Regularly Interspaced Short Palindromic Repeats Complex Data Dedications Disabled Persons Disease Environment Environmental Exposure Environmental Impact Environmental Pollutants Environmental Risk Factor Exposure to Gene Expression Genes Genetic Genetic Diseases Genetic Transcription Goals Health Hereditary Disease Heterogeneity Human Individual Introns Laboratories Length Libraries Mediating Mendelian disorder Mentorship Motor Mutation Neurodegenerative Disorders Phenotype Population Recording of previous events Research Research Assistant Resources Role Spinal Ganglia Stress Students System Training Trinucleotide Repeats Undifferentiated Variant Work autosome candidate identification career career development cell type differential expression disability disabled students experimental study frataxin gene correction gene environment interaction gene product genetic variant genome wide association study graduate student induced pluripotent stem cell insight loss of function mutant novel parent grant resilience response screening single-cell RNA sequencing stressor toxicant transcriptome sequencing vector

Afferent Neurons Alleles Ataxia CRISPR library CRISPR screen Candidate Disease Gene Cardiac Myocytes Categories Cell Line Cell model Cells Characteristics Chromosome Mapping Clustered Regularly Interspaced Short Palindromic Repeats Complex Data Dedications Disabled Persons Disease Environment Environmental Exposure Environmental Impact Environmental Pollutants Environmental Risk Factor Exposure to Gene Expression Genes Genetic Genetic Diseases Genetic Transcription Goals Health Hereditary Disease Heterogeneity Human Individual Introns Laboratories Length Libraries Mediating Mendelian disorder Mentorship Motor Mutation Neurodegenerative Disorders Phenotype Population Recording of previous events Research Research Assistant Resources Role Spinal Ganglia Stress Students System Training Trinucleotide Repeats Undifferentiated Variant Work autosome candidate identification career career development cell type differential expression disability disabled students experimental study frataxin gene correction gene environment interaction gene product genetic variant genome wide association study graduate student induced pluripotent stem cell insight loss of function mutant novel parent grant resilience response screening single-cell RNA sequencing stressor toxicant transcriptome sequencing vector

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项目摘要

Summary The goal of this supplement is to enhance diversity in the research workforce through support of a disabled graduate student in response to PA-21-071(Research Supplement to Promote Diversity in Health-Related Research). This supplement will enable the disabled student to work in the laboratory of the PI of parent grant by providing support for his stipend and tuition, as well as allowing the hiring of a dedicated technical research assistant for the components of the proposed work requiring fine motor capabilities. In addition, mentorship, career development support, targeted training efforts, and reasonable accommodations will enable the student to continue to pursue a scientific career while navigating the challenges presented by his disability. The parent grant focuses on GXE interactions with exogenous stressors but posited application to GXE interactions with endogenous stressors, such as a pre-existing monogenic genetic disorder, which is the focus of the supplement. We selected the monogenic disorder, Friedrich’s ataxia (FRDA), a progressive neurodegenerative disorder most commonly due to triplet repeat mutations in the frataxin gene. While triplet repeat length plays a role in the disease presentation, other unknown genetic and environmental factors clearly contribute. We will assess the capability of our novel targeted functional screening approach to identify genes which when disrupted could modulate the cellular phenotype of FRDA. As with the parent grant, the work will focus on the set of ~1490 genes with common LOF mutations in the population and will assess functional effect by assessing changes in the transcriptional phenotype of relevant cells. This work if successful could provide important insight into common LOF variants that impact the presentation and progression of Friedrich’s ataxia. This functional screening approach using transcriptional phenotypic endpoints could have general applicability to any genetic disease and enable identification of potentially functionally significant and population relevant genetic variants impacting the phenotypic manifestations of the disease.

概括这种补充的目的是通过支持残疾人来增强研究劳动力的多样性研究生响应PA-21-071（促进与健康相关的多样性的研究补充研究）。这种补充剂将使残疾学生能够在父母赠款的PI实验室工作通过为他的压力和学费提供支持，并允许雇用专门的技术研究需要精细运动能力的拟议工作组成部分的助手。此外，Mentalship，职业发展支持，有针对性的培训工作以及合理的住宿将使学生能够继续从事科学职业，同时导致其残疾带来的挑战。父母格兰特专注于与外源压力源的GXE相互作用，但已发布到与GXE相互作用的应用内源性应激源，例如先前存在的单基因遗传疾病，这是补充剂的重点。我们选择了单基因疾病，弗里德里希共济失调（FRDA），这是一种进行性神经退行性障碍通常是由于Frataxin基因中的三胞胎重复突变引起的。而三胞胎重复长度在疾病表现，其他未知的遗传和环境因素显然促成。我们将评估我们新颖的靶向功能筛选方法的能力鉴定基因，当受到干扰时调节FRDA的细胞表型。与父母赠款一样，工作将重点放在〜1490的集合上人群中具有常见LOF突变的基因，将通过评估变化来评估功能效应相关细胞的转录表型。这项工作如果成功可以提供重要的见解影响弗里德里希共济失调的表现和发展的常见LOF变体。这个功能使用转录表型端点的筛选方法可能对任何通用的一般适用性疾病并能够鉴定潜在的功能意义和种群相关的遗传变异影响疾病的表型表现。