Equipment Core

装备核心

• 批准号: 7616251
• 负责人: RICHARD BRIAN DYER
• 金额: $ 14.43万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7616251
• 关键词: Equipment; Fluorescence; Heating; Isotopes; Kinetics; Lasers; Measurement; Nature; Optics; Proteins; Pump; Range; Relaxation; Research Personnel; Resolution; Signal Transduction; Solutions; Spectrum Analysis; Standards of Weights and Measures; System; Temperature; Time; Water; Work; absorption; base; instrument; irradiation; programs; protein structure; research study; size; temperature jump

Core B - 'The Equipment Core'. The 'Equipment Core' supports the major equipment used to characterized the dynamical nature of the protein systems of the various projects of the Program Project. Two spectroscopic approaches are supported: 1. Temperature-jump relaxation spectroscopy. Three instruments are employed to cover the entire time range from 10 ps to minutes (or longer) in three overlapping time domains. (a) 10 ps - 10 ns. The T-jump is performed by heating water by laser irradiation, and the resolution of the instrument is set by the thermalization time of the absorbed energy. This picosecond instrument is located at Los Alamos. Both IR and UV/Vis absorbance and emission can be performed as probes of protein structure. (b) 15 ns-10 ms. The T-jump is achieved by heating water by laser irradiation. Three separate spectrometers will be employed. Two instruments are located at Albert Einstein and perform UV/Vis absorbance and/or fluorescence. IR transient studies will be performed at Los Alamos. (c) 1 ms - minutes (or longer). This instrument will be purchased and based at Einstein. The T-jump is achieved by mixing two solutions at different temperatures and holding the mixed solution at a final temp. Optical (UV/Vis) emission and or absorbance and IR absorbance spectroscopies are employed as structural probes. 2. A static isotope editing IR difference spectrometer located at Albert Einstein. In general, static thermal measurements precede T-jump kinetic studies. These calibrate the expected signal size to be observed in the kinetic work and also sets the magnitude of signal at infinite time in the kinetics experiments.

核心B-“设备核心”。 “设备核心”支持用于表征程序项目各个项目蛋白质系统的动力学性质的主要设备。支持两种光谱法： 1。悬浮的松弛光谱。在三个重叠的时间域中，使用三种仪器来覆盖从10 ps到分钟（或更长）的整个时间范围。 （a）10 ps -10 ns。通过激光照射通过加热水进行T-跳跃，仪器的分辨率是通过吸收能量的热化时间来设定的。这款Picsecond乐器位于Los Alamos。 IR和UV/Vis吸光度和发射都可以作为蛋白质结构的探针进行。 （b）15 ns-10 ms。通过激光照射加热水来实现T-跳跃。三个分开 光谱仪将被使用。两种仪器位于阿尔伯特·爱因斯坦（Albert Einstein），并执行紫外线/或荧光。 IR瞬态研究将在Los Alamos进行。 （c）1毫秒 - 分钟（或更长的时间）。该乐器将在爱因斯坦购买并基于爱因斯坦。通过在不同温度下混合两种溶液并将混合溶液保持在最终温度下，可以实现T突变。光学（UV/VIS）发射和/或吸光度和IR吸光度光谱被用作结构探针。 2。位于阿尔伯特爱因斯坦的静态同位素编辑IR差异光谱仪。通常，静态热测量在T-跳动动力学研究之前。这些校准了动力学工作中要观察到的预期信号大小，并在动力学实验中设置了无限时间的信号幅度。