Human Embryonic Stem Cell Training and Culture Core

人类胚胎干细胞训练和培养核心

• 批准号: 7678453
• 负责人: MARGARET A. GOODELL
• 金额: $ 26.77万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7678453
• 关键词: Adoption; Biology; Cell Line; Cells; Collaborations; Communities; Continuing Education; Data; Educational process of instructing; Educational workshop; Embryo; Ensure; Extracellular Matrix; Fibroblasts; Freezing; Genetic; Geographic Locations; Growth Factor; Hand; Human; Immunophenotyping; In Situ; In Vitro; Instruction; Karyotype determination procedure; Laboratories; Maintenance; Malignant Neoplasms; Manuals; Medical center; Medicine; Modification; Mus; Mycoplasma; Numbers; Pilot Projects; Preparation; Quality Control; Reagent; Research Personnel; Resources; Rice; Sampling; Scientist; Serum; Services; Sterility; Techniques; Technology; Teratoma; Testing; Texas; Training; Training Activity; Training Programs; Universities; WA01 cell line; WA09 Cell Line; Week; Work; base; college; cost; experience; gulf coast; human embryonic stem cell; human embryonic stem cell line; in vivo; pluripotency; programs; reagent testing; stem

This human Embryonic Stem (hES) Cell Training and Culture Core has two objectives. The first is to provide centralized hES culture support to all projects; the second is to broadly support hES cell work in our geographic region (Texas/Gulf Coast/South-west) by sponsoring a hES cell training program, to facilitate adoption of the technology among a larger number of laboratories. For the first objective, we will provide quality-tested reagents, such as lot-tested media and mouse embryonic fibroblasts (MEF) to all projects. We will also provide cultures of WA01 (H1)and WA09 (H9)cells to project investigators on a routine basis, and large-scale cultures of these lines upon request. We will ensure the hES provided to our investigators are high quality through routine testing for sterility, pluripotency, and genetic stability. For the second objective, we will offer a hES Training workshop every other month. This workshop will be an intensive week-long hands-on training in small groups with highly experienced hES workers. Training will include all basic hES-related technologies, such as MEF preparation and quality testing, hES culture and quality analysis, teratoma formation, and EB differentiation. In addition, an extended workshop will be offered annually that will include strategies for genetic modification. This workshop will be taught in collaboration with the Genetic Modification Core (Core C). By centralizing these efforts of hES training and culture, we expect to ensure high quality hES culture for our investigators, as well as dissemination of the technology throughout the Texas Medical Center and larger geographic region.

这种人类胚胎（HES）细胞训练和培养核心有两个目标。首先是 为所有项目提供集中的HES文化支持；第二个是广泛支持HES细胞工作 我们的地理区域（德克萨斯州/墨西哥湾沿海/西南）通过赞助HES Cell培训计划， 促进在大量实验室中采用该技术。对于第一个目标，我们将 向所有人提供质量测试的试剂，例如经过大量测试的培养基和老鼠胚胎成纤维细胞（MEF） 项目。我们还将提供WA01（H1）和WA09（H9）细胞的培养物，以投影研究人员 根据要求，常规的基础和这些线的大规模文化。我们将确保提供给他的 我们的研究人员通过常规测试对不育，多能性和遗传稳定性来提高质量。 对于第二个目标，我们将每隔一个月提供一次HES培训研讨会。这个研讨会将 在与经验丰富的HE工人的小组中进行长达一周的深入动手培训。 培训将包括所有与HES相关的基本技术，例如MEF准备和质量测试，HES 培养和质量分析，畸胎瘤形成和EB分化。此外，扩展 每年将提供研讨会，其中包括遗传修饰的策略。这个研讨会将 与遗传修饰核心（核心C）合作教授。通过集中这些努力 他的培训和文化，我们希望确保调查人员以及 整个得克萨斯州医疗中心和更大的地理区域的技术传播。