Hedgehog signaling during cardiovascular patterning in the mouse

小鼠心血管模式中的刺猬信号传导

• 批准号: 7567524
• 负责人: JOHN A KLINGENSMITH
• 金额: $ 39万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7567524
• 关键词: Adult; Affect; Anterior; Cardiac; Cardiovascular system; Cells; Complex; Congenital Heart Defects; Data; Defect; Development; Double Outlet Right Ventricle; Embryo; Embryonic Heart; Endocardium; Endoderm; Erinaceidae; Excision; Heart; In Vitro; Ligands; Mesenchymal; Mitral Valve; Molecular; Mus; Myocardial; Myocardium; Neural Crest Cell; Pattern; Persistent Truncus Arteriosus; Population; Pulmonary artery structure; Research Personnel; Right ventricular structure; SHH gene; Signal Pathway; Signal Transduction; Staging; Testing; Tissues; Tricuspid valve structure; Ventricular; cardiogenesis; congenital heart disorder; genetic manipulation; human SMO protein; in vivo; insight; malformation; mutant; precursor cell; programs; receptor; research study; smoothened signaling pathway

DESCRIPTION (provided by applicant): The outflow tract of the embryonic heart contributes to the formation of the aortic and pulmonary artery. Defects in outflow tract development result in severe congenital heart defects such as persistent truncus arteriosus and double outlet right ventricle. Similarly, atrio-ventricular (A-V) cushions contribute to the formation of the mitral and tricuspid valves as well as to the septa of the adult heart. Abnormalities in A-V cushion development can result in severe congenital heart defects such as A-V canal or tricuspid and mitral valve atresias. Understanding how the outflow tract forms and the A-V cushions develop is therefore critical to understanding the development of many congenital heart defects. Classic studies have demonstrated the requirement of cardiac neural crest cells (CNCC) in the septation of the single outflow tract into the aortic and pulmonary arteries. More recent studies have provided new insight into the development of the myocardium of the outflow tract of the heart. These studies demonstrate the existence of heart precursor cells that are added to the outflow tract and right ventricle. Our preliminary data suggests that the Shh signaling pathway is critical for these "anterior heart field" (AHF) cells as well as for migratory CNCC during outflow tract formation and septation. In addition, Shh appears to be required for A-V cushion formation and therefore valve development. Loss of the Shh signal results in a single outflow tract (pulmonary artery atresia) and a single A-V valve. We propose that the cardiac defects seen in Shh mutants are due to abnormal development of both CNCC and the AHF during outflow tract development as well as abnormal endocardial cushion formation during valve formation. We propose examining the cell autonomous requirement for hedgehog signaling within AHF and CNCC in the coordinated development of the outflow tract using a Cre/LoxP approach. Similarly we will test the cell autonomous requirement of hedgehog signaling during A-V valve formation using genetic manipulations in the mouse.

描述（由申请人提供）：胚胎心脏的流出道有助于主动脉和肺动脉的形成。流出道发育的缺陷导致严重的先天性心脏缺陷，例如持续的Truncus Arteriosus和Double Outlet右心室。同样，心室（A-V）垫子有助于形成二尖瓣和三尖瓣的形成以及成人心脏的隔膜。 A-V缓冲发育的异常会导致严重的先天性心脏缺陷，例如A-V运河或三尖瓣和二尖瓣闭锁。因此，了解流出道的形成和A-V垫子如何发展对于理解许多先天性心脏缺陷的发展至关重要。经典研究表明，在单一流出道中分离到主动脉和肺动脉中，心脏神经rest细胞（CNCC）的要求。最近的研究为心脏流出道的心肌发展提供了新的见解。这些研究表明，添加到流出道和右心室中的心脏前体细胞的存在。我们的初步数据表明，SHH信号通路对于这些“前心脏场”（AHF）细胞以及在流出道形成和分离期间的迁移CNCC至关重要。此外，A-V缓冲层形成和阀发育似乎是SHH。 SHH信号的损失导致单一流出道（肺动脉闭锁）和单个A-V阀。我们建议在SHH突变体中看到的心脏缺陷是由于流出道发育过程中CNCC和AHF的异常发育以及瓣膜形成期间异常的心内膜垫形成。我们建议使用CRE/LOXP方法在AHF和CNCC中检查AHF和CNCC内的刺猬信号传导的细胞自主需求。同样，我们将使用小鼠中的遗传操作来测试A-V阀形成过程中刺猬信号传导的细胞自主需求。