Pet Core Facility

宠物核心设施

• 批准号: 7599236
• 负责人: Alan J Fischman
• 金额: $ 12.57万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7599236
• 关键词: Animal Experimentation; Animals; Apoptosis; Arts; Biological Process; Burn Trauma; C57BL/6 Mouse; Complex; Core Facility; Data Analyses; Development; Gene Expression; Genetic; Hospitals; Human; Human Volunteers; Image; Imaging Techniques; Immune response; Inflammatory; Intensive Care; Intervention; Label; Laboratory Animals; Life; Methodology; New Agents; Nuclear; Patients; Physiological; Positron-Emission Tomography; Production; Protocols documentation; Range; Research Personnel; Resolution; Services; Skeletal Muscle; Support of Research; Technology; Testing; Time; Tissues; bone imaging; design; human subject; instrumentation; molecular imaging; new technology; trauma centers

The PET and muPET Facility Core provides the Burn Trauma Center with the capability to apply nuclear imaging techniques to study phenomena at the tissue, cellular, and genetic levels. The facility core supports research that allows us to study a wide range of biological processes in both humans and living laboratory animals. In particular, this technology using either state-of-the-art conventional PET cameras or a muPET camera provides us with the ability to study an animal more than once, allows each animal to serve as its own control, and allows interventional strategies to be followed over time. Furthermore, identical studies can also be conducted non-invasively directly in normal human volunteers and patients. The muPET camera, which enables imaging at resolutions of 1 mm or less in both humans and animals, is mobile and will make imaging skeletal muscle possible in our intensive care patients while they are still acutely ill. Further development of molecular imaging methodologies with high resolution will enable investigators to image gene expression in relevant tissues and skeletal muscle apoptosis to better understand the immuno-inflammatory host response initially in C57BL/6 mice and later in humans. The PET and muPET Facility Core allows complex studies to be performed within the Human Subjects Core and Animal Research Cores at the MGH, Shriners Hospital, and MIT. The facility core provides the Burn Trauma Center investigators with the capability to apply PET technology to test scientific hypotheses without the need to become PET experts themselves. The PET and muPET Facility Core offers several services to the Burn Trauma Center. (1) Design and development of new physiological imaging agents labeled with 18F, 11C, 13N, or 15O. (2) Routine Production and dispensing of existing and new imaging agents for PET study protocols. (3) Design of imaging and data analysis protocols for new agents. (4) Routine execution of existing imaging protocols and data analyses. (5) Development of new instrumentation for specific applications within the Center (e.g. mu PET camera). (6) Development of new technology for real-time molecular imaging of apoptosis and gene expression. Support is requested in this facility for methodology and development that will be very helpful in all four projects.

PET和MUPET设施核心提供了燃烧创伤中心，具有应用核成像技术来研究组织，细胞和遗传水平的现象的能力。设施核心支持的研究使我们能够研究人类和活物动物的广泛生物学过程。特别是，这项使用最先进的传统宠物摄像机或mupet相机的技术使我们能够多次研究动物，允许每只动物作为自己的控制，并允许随着时间的推移遵循介入的介入策略。此外，同一研究也可以直接在正常的人类志愿者和患者中进行非侵入性进行。 Mupet摄像机可以在人类和动物中以1毫米或更少的分辨率进行成像，并且在我们的重症监护患者仍然急性病的同时，将使成像骨骼肌成为可能。具有高分辨率的分子成像方法的进一步发展将使研究人员能够在相关组织和骨骼肌凋亡中对基因表达进行图像，以更好地了解最初在C57BL/6小鼠中以及后来在人类中的免疫炎症宿主反应。 PET和MUPET设施核心允许在MGH，Shriners医院和MIT的人类受试者核心和动物研究核心内进行复杂的研究。 设施核心为烧伤创伤中心调查人员提供了应用PET的能力 研究科学假设的技术，而无需成为宠物专家。宠物和mupet设施核心为烧伤创伤中心提供了多种服务。 （1）用18F，11C，13N或15O标记的新生理成像剂的设计和开发。 （2）宠物研究方案的现有和新成像剂的常规生产和分配。 （3）新代理的成像和数据分析协议的设计。 （4）例行执行现有成像协议和数据分析。 （5）开发中心内特定应用的新仪器（例如MU PET相机）。 （6）开发凋亡和基因表达的实时分子成像的新技术。在此设施中要求提供支持，以在所有四个项目中都非常有帮助。