Genome and epigenome editing of induced pluripotent stem cells for investigating osteoarthritis risk alleles

诱导多能干细胞的基因组和表观基因组编辑用于研究骨关节炎风险等位基因

基本信息

批准号：
10707979
负责人：
Farshid Guilak
金额：
$ 17.11万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-22 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10707979
关键词：
Acceleration Affect Alleles Anabolism Automobile Driving Biochemical Biological Models CRISPR/Cas technology Cartilage Cartilage Matrix Cartilage injury Catabolism Chondrocytes Chromosome 8 Complex Cytoskeletal Proteins DNA Methylation Data Degenerative polyarthritis Development Epigenetic Process Equilibrium Event Future Gene Frequency Genes Genetic Genetic Polymorphism Genetic Risk Genetic Variation Genome Genotype Goals Hip Osteoarthritis Histology Homeostasis Human Human Engineering Hypertrophy Immunohistochemistry In Vitro Introns Knock-out Lead Link Mechanical Stress Mechanics Mediating Methylation Minor Molecular Pathologic Pathway interactions Patients Phenotype Physiological Physiology Play Process Production Quantitative Trait Loci Regulation Regulatory Pathway Reporting Risk Role Secondary to Signal Pathway Signal Transduction Single Nucleotide Polymorphism Specimen System Testing Tissue Engineering Tissues United Kingdom articular cartilage biobank cartilage development cell type cohort comparison control disability epigenetic regulation epigenome epigenome editing gene regulatory network genetic variant genome editing genome wide association study in vitro Model induced pluripotent stem cell interest mechanical load mechanical properties mechanotransduction new therapeutic target novel plectin prevent response risk variant single-cell RNA sequencing stem cells transcriptome transcriptome sequencing variant of interest

项目摘要

Abstract Genome wide association studies (GWAS) in large cohorts have identified and replicated robust single nucleotide polymorphisms (SNPs) with a strong association to osteoarthritis (OA) development. While many SNPs have been identified as risks for OA development, a mechanistic understanding of their role in cartilage homeostasis and mechanobiology has remained elusive. We propose to use a novel in vitro system combining genome editing of human induced pluripotent stem cells (iPSCs) and cartilage tissue engineering for studying the functional effect of identified OA SNPs on the biochemical and mechanical properties of articular cartilage. In preliminary data, we reported a functional characterization of rs11780978 in which we identified an expression quantitative trait locus (eQTL) operating on the gene PLEC in the cartilage of patients with OA. Our primary hypothesis is that OA-associated SNP rs11780978 results in reduced expression of PLEC in chondrocytes secondary to altered epigenetic regulation at this locus. Using CRISPR-Cas9 and dCas9TET-mediated genome editing, we will examine the role of genetic and epigenetic modulation (PLEC knockout and hypomethylation of the functional region) on the chondrogenic differentiation of hiPSCs. Furthermore, we propose that reduced production of plectin due to this SNP alters the response of chondrocytes to mechanical stress under physiologic or pathologic conditions. This tissue-engineering approach provides a model system that can be used in future studies to examine specific mechanistic hypothesis on the link between OA-associated SNPs and the regulation of chondrocyte physiology. Identification of these mechanisms will hopefully lead to new therapeutic targets for preventing or slowing OA progression.

抽象的大型队列中的基因组广泛关联研究（GWAS）已鉴定并复制了可靠的单一核苷酸多态性（SNP）与骨关节炎（OA）发育有很强的关联。而很多 SNP已被确定为OA开发的风险，一种机械理解其在软骨中的作用稳态和机械生物学仍然难以捉摸。我们建议使用一种新颖的体外系统结合人类诱导多能干细胞（IPSC）和软骨组织工程的基因组编辑用于研究已鉴定的OA SNP对生化和机械性能的功能效应关节软骨。在初步数据中，我们报告了RS11780978的功能表征，其中我们鉴定出在患者软骨中在基因PLEC上作战的表达定量性状基因座（EQTL）与OA。我们的主要假设是，与OA相关的SNP RS11780978导致表达降低在该基因座的表观遗传调节变化的软骨细胞中PLEC。使用CRISPR-CAS9和 DCAS9TET介导的基因组编辑，我们将研究遗传和表观遗传调节的作用（PLEC 功能区域的敲除和甲基化）在HIPSC的软骨分化上。此外，我们提出，由于SNP，降低了蛋白质的产生，改变了在生理或病理状况下机械应力的软骨细胞。这种组织工程方法提供了一个模型系统，可以在以后的研究中使用该系统来检查特定的机理关于OA相关SNP与软骨细胞生理学调节之间联系的假设。这些机制的识别将导致预防或放缓OA的新治疗靶点进展。