Botanical Core

植物核心

• 批准号: 10671058
• 负责人: Amar G. Chittiboyina
• 金额: $ 20.43万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10671058
• 关键词: Botanical; Core

BOTANICAL CORE: ABSTRACT/SUMMARY The overall purpose of the Botanical Core of the proposed University of Mississippi (UM) Botanical Dietary Supplements Research Center (BDSRC) is to ensure product integrity and advance chemistry research on the Spirulina-based product, Immulina™, using a combination of bioassay- and chemical-based approaches. Researchers affiliated with the proposed UM BDSRC have generated evidence that supports reproducible and mechanistically plausible effects of Immulina™ for enhancing host responses that may promote resilience against respiratory viral infections such as influenza which resulted in 959,000 patient hospitalizations and 79,400 deaths in United States alone. The Botanical Core will be headed by Amar Chittiboyina, PhD, a medicinal chemist with extensive research experience in natural product chemistry as well as standardization methodologies. The specific aims of the Botanical Core are to: 1. Ensure the unambiguous identification and develop additional safety testing on Arthrospira (Spirulina) platensis raw material that will be used in the production of sufficient quantities of Immulina™ for the proposed research projects; 2. Develop and validate a selective in vitro bioassay for quantitation of the toll-like receptor 2-dependent activity exhibited by the Braun-type lipoproteins in Immulina™; 3. Advance chemistry research on Immulina™ by establishing chemical-based authentication and standardization approaches, characterization of immune-inhibitory substances and detailed structural analysis of the active immune-enhancing Braun-type lipoproteins; and 4. Perform additional stability studies, provide sufficient quantities of well-characterized and safe ImmulinaTM for use in the proposed UM BDSRC research projects, and explore collaborative opportunities with other CARBON units. Expected outcomes include (1) achieving a higher level of product integrity and quality assurance of Immulina™ used in the proposed UM BDSRC projects; (2) design of an improved biological standardization method that will selectively detect only TLR2 agonists such as the Braun-type lipoproteins in Immulina™; (3) providing chemical-based authentication and standardization methods that will complement the bioassay-based standardization method and provide a robust system to fully characterize Immulina™ product material; and (4) to provide sufficient quantities of standardized Immulina™ working closely with other researchers from the UM BDSRC and collaborating with other units of CARBON on future efficacy studies of ImmulinaTM.

植物核心：摘要/摘要 密西西比大学植物学的植物核心（UM）植物饮食的总体目的 补充剂研究中心（BDSRC）是为了确保产品完整性并提高化学研究 基于螺旋藻的产品Immulina™，结合了生物测定方法和基于化学的方法。 研究人员隶属于拟议的UM BDSRC，已经产生了支持可再现和的证据 Immulina™在机械上合理的效果增强了可能促进弹性的宿主反应 反对呼吸道病毒感染，例如影响力，导致959,000例患者住院和 仅美国就有79,400人死亡。 植物核心将由Amar Chittiboyina博士领导，该博士是一名具有广泛研究的医学化学家 自然产品化学和标准化方法的经验。 植物核心的具体目的是： 1。确保明确的识别并开发有关关节螺旋藻（Spirulina）的其他安全测试 Platensis原材料将用于生产足够数量的Immulina™的原材料 拟议的研究项目； 2。开发和验证选择性的体外生物测定方法，以定量类似收费的接收器2依赖性 Immulina™中Braun型脂蛋白暴露的活性； 3。通过建立基于化学化学的身份验证和 标准化方法，免疫抑制物质的表征和详细的结构 分析活性免疫增强的Braun型脂蛋白；和 4.进行额外的稳定性研究，提供足够数量的特征和安全 用于拟议的UM BDSRC研究项目中的iMmulinatm，并探索协作 其他碳单元的机会。 预期结果包括（1）实现更高水平的产品完整性和质量保证 Immulina™用于拟议的UM BDSRC项目； （2）改进的生物标准化的设计 仅选择性检测TLR2激动剂（例如Immulina™中的Braun型脂蛋白）的方法； （3） 提供基于化学的身份验证和标准化方法，该方法将完成基于生物测定的标准化方法，并提供强大的系统以充分表征Immulina™产品材料； （4）提供足够数量的标准化Immulina™与其他研究人员紧密合作 UM BDSRC并与其他碳的其他单位合作在未来的免疫效率研究中进行。