EFFECTS OF MERCURIC CHLORIDE ON MALE REPRODUCTION

氯化汞对男性生殖的影响

• 批准号: 7561453
• 负责人: HARI Om GOYAL
• 金额: $ 9.57万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561453
• 关键词: Affect; Age; Computer Retrieval of Information on Scientific Projects Database; Control Groups; Development; Environment; Female; Fertility Rates; Fetus; Funding; Grant; Ingestion; Institution; Mercuric chloride; Mercury; Numbers; Ovulation; Ovum; Partner in relationship; Pregnancy; Rattus; Reproduction; Research; Research Personnel; Resources; Side; Source; Staging; United States National Institutes of Health; Uterus; Water; Weight Gain; assault; corpus luteum; day; implantation; male

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this study was to investigate effects of mercuric chloride (MC) ingestion on the fertility rate of female rats including (1) the number of viable fetuses, (2) the number of implantations, and (3) the number of corpora lutea. Female rats, 102 days of age, were treated with 2.5 mg/kg/day of MC (treatment group) or de-ionized water (control group) for a period of 60 days. They were then mated with 70 to 80-day-old untreated males (2 females to 1 male) for 17 days. Pregnancy was confirmed by weight increase. Upon confirmation of pregnancy, all females were euthanized and examined for the number of viable fetuses, implantations, and corpora lutea in both sides. The mercury treatment resulted in lowering the mean number of fetuses (6.9 vs 12.4 in controls) and implantations (10.5 vs 12.8 in controls); however, the decrease was significant (P less than 0.05) only in the case of fetuses. Conversely, the number of corpora lutea was not different between the treated and control groups (14.9 vs 14.8 in controls). Hence, it is hypothesized that the mercury-induced reduction in the number of fetuses resulted at a post-implantation stage; probably from a malfunction of the uterus so that it could not provide a proper environment for the development of the fetus, or from a direct assault of mercury on the fetus, or both. It is further hypothesized that mercury ingestion did not affect ovulation since the number of corpora lutea (indicator for the number of ova released) was not different between the treated and control groups.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 本研究的目的是调查氯化汞 (MC) 摄入对雌性大鼠生育率的影响，包括 (1) 存活胎儿数量、(2) 着床数量和 (3) 黄体数量。 102天龄的雌性大鼠用2.5mg/kg/天的MC（治疗组）或去离子水（对照组）治疗60天。然后将它们与 70 至 80 天大的未经处理的雄性（2 雌性对 1 雄性）交配 17 天。通过体重增加证实怀孕。确认怀孕后，对所有雌性实施安乐死，并检查两侧的存活胎儿数、植入数和黄体数。 汞处理导致平均胎儿数量（对照组为 6.9 vs 12.4）和植入数（对照组为 10.5 vs 12.8）降低；然而，只有胎儿的下降才显着（P 小于 0.05）。相反，治疗组和对照组之间的黄体数量没有差异（对照组为 14.9 vs 14.8）。 因此，推测汞引起的胎儿数量减少是在着床后阶段造成的；可能是由于子宫功能障碍，无法为胎儿的发育提供适当的环境，或者是汞对胎儿的直接攻击，或者两者兼而有之。 进一步假设汞摄入不会影响排卵，因为治疗组和对照组之间的黄体数量（卵子释放数量的指标）没有差异。