Virus & Reservoirs Core

病毒

• 批准号: 10656366
• 负责人: Katharine June Bar
• 金额: $ 34.97万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10656366
• 关键词: AIDS Vaccines; Abbreviations; Acquired Immunodeficiency Syndrome; Advisory Committees; Antigens; Area; Basic Science; Bioinformatics; Biological Assay; Budgets; Cell Therapy; Cells; Clinical; Clinical Trials; Collaborations; Communities; Complement; Complex; Consultations; Custom; DNA; Development; Education; Emerging Technologies; Experimental Designs; Feedback; Foundations; Funding; Generations; Genome; Goals; HIV; HIV vaccine; HIV/AIDS; Immunology; Laboratory Research; Lead; Leadership; Mentors; Mentorship; Mission; Modeling; Molecular; Molecular Cloning; Molecular Virology; Pathogenesis; Peripheral Blood Mononuclear Cell; Phenotype; Population; Positioning Attribute; Prevention; RNA; Reagent; Reporting; Research; Research Personnel; Research Priority; Retroviral Vector; SIV; Scientist; Series; Services; Strategic Planning; Structure; Techniques; Technology; Therapy Clinical Trials; Time; Tissues; Training; Training and Education; Transcript; Translational Research; United States National Institutes of Health; Vaccines; Viral; Viral Load result; Viral Proteins; Viral reservoir; Virus; Work; antiretroviral therapy; career; deep sequencing; density; design; digital; first-in-human; gene therapy; innovation; integration site; member; new technology; next generation; nonhuman primate; novel; novel strategies; outreach; preclinical study; programs; repository; response; screening; service programs; simian human immunodeficiency virus; single cell analysis; transmission process; vaccine discovery; viral DNA; working group; zinc finger nuclease

Core D: Virus and Reservoirs ABSTRACT The mission of the Virus and Reservoirs Core (Core D) is to provide unparalleled molecular virology and sequencing support, services, facilities, consultation, and training to Penn CFAR investigators on campus, as well as the HIV/AIDS research community more broadly. Through this cutting-edge enabling technology combined with proactive scientific leadership, Core D seeks to advance AIDS research in high priority scientific directions. In the current reporting period, Core D has made substantial changes to both meet evolving needs, opportunities and priorities, and lead a forward-looking research agenda in the area of HIV cure, pathogenesis understanding of persistent viral reservoirs. These changes include new services as well as installation of a new leadership team comprised of Drs. Katharine Bar (Director) and Mohamed Abdel- Mohsen (Co-Director), and three senior HIV/AIDS scientists as Core Investigators (Drs. Frederic Bushman, Beatrice Hahn and George Shaw) to provide both specialized services and mentorship to the leadership team. To support its mission, the Virus and Reservoirs Core provides: (1) State-of-the-art molecular virology services including an extensive virus repository, viral assay services for economy of scale, an innovative cell product release assay for retroviral vector transduced ex vivo expanded cells to support innovative gene- and cell-therapy clinical trials, and quantitative real-time and digital droplet PCR platforms; (2) State-of-the-art sequencing services with analytical support, including Single Genome Amplification, amplicon sequencing by Illumina and Sanger platforms with custom processing and analytic support, and deep sequencing for complex populations or integration site analysis; (3) A new service based on novel transmitted/founder SHIV (SIV/HIV chimeric) viruses, to leverage the power of a new generation of SHIVs with tremendous opportunity for vaccine, pathogenesis and cure research; (4) Novel platforms for highly sensitive and specialized viral analysis including quantification of viral transcripts, proviral integration, single-copy viral load, single-cell analysis and related techniques; (5) Mentoring and education in appropriate application of these emerging technologies as well as training in implementation; and (6) Proactive outreach and leadership to promote research that exploits these technologies, including close collaboration with the HIV Reservoirs & Tissue Immunology SWG. In the current reporting period, Core D provided support to 119 investigators, generated over $5,000,000 in chargebacks, and supported more than 140 NIH-funded applications. Looking ahead to the upcoming cycle, in close collaboration with the Clinical, Immunology and Nonhuman Primate Cores and the Reservoirs and Tissue Immunology SWG, Core D will expand its services to galvanize basic and translational laboratory research, pre- clinical studies in nonhuman primates, and first-in-human clinical trials studying HIV reservoirs, virus persistence, and cure strategies.

核心D：病毒和水库 抽象的 病毒和储层核心（核心D）的任务是提供无与伦比的分子病毒学和 对校园宾夕法尼亚州CFAR调查人员进行测序，服务，设施，咨询和培训， 以及更广泛的艾滋病毒/艾滋病研究界。通过这项尖端的促成技术 结合积极主动的科学领导，核心D试图以高优先级的科学发展促进艾滋病研究 方向。在当前的报告期间，核心D已实质性更改以满足不断发展的 需求，机会和优先事项，并领导着艾滋病毒治愈领域的前瞻性研究议程， 对持续病毒储层的发病机理理解。这些更改还包括新服务 作为由DRS组成的新领导团队的安装。 Katharine Bar（导演）和穆罕默德·阿卜杜勒（Mohamed Abdel） Mohsen（联合导演）和三名高级艾滋病毒/艾滋病科学家担任核心研究人员（弗雷德里克·布什曼（Frederic Bushman）博士， 比阿特丽斯·哈恩（Beatrice Hahn）和乔治·肖（George Shaw））为领导团队提供专业服务和指导。 为了支持其使命，病毒和水库核心提供：（1）最先进的分子病毒学服务 包括广泛的病毒存储库，用于规模经济的病毒测定服务，一种创新的细胞产品 逆转录病毒载体转导的离体扩张细胞的释放测定，以支持创新的基因和细胞疗法 临床试验以及定量实时和数字液滴PCR平台； （2）最先进的测序 具有分析支持的服务，包括单个基因组扩增，Illumina和 具有自定义处理和分析支持的Sanger平台，以及对复杂人群的深入测序 或集成站点分析； （3）基于新型传输/创始人SHIV（SIV/HIV嵌合）的新服务 病毒，利用新一代湿婆的力量，为疫苗带来巨大机会， 发病机理和治愈研究； （4）高度敏感和专业的病毒分析的新型平台，包括 量化病毒转录本，前病毒整合，单拷贝病毒载荷，单细胞分析和相关 技术； （5）在适当应用这些新兴技术的指导和教育以及 实施培训； （6）积极主动的宣传和领导层以促进利用这些研究的研究 技术，包括与HIV水库和组织免疫学SWG密切合作。 在当前的报告期间，核心D向119名调查人员提供了支持，产生了超过5,000,000美元 退款，并支持140多个NIH资助的申请。展望即将到来的周期， 与临床，免疫学和非人类灵长类核心以及储层和组织密切合作 Immunology SWG，核心D将扩大其服务，以激发基础和转化实验室研究， 非人类灵长类动物的临床研究以及研究HIV储层，病毒持久性的第一临床临床试验， 和治愈策略。