Protective film-forming disinfectant based on chitosan/water/ethanol tertiary solutions

基于壳聚糖/水/乙醇三元溶液的保护膜消毒剂

基本信息

批准号：
10662022
负责人：
Alexey A Vertegel
金额：
$ 5.5万
依托单位：
VRM LABS INC
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-13 至 2023-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10662022
关键词：
2019-nCoV Affect Aftercare Alcohols American Ammonium Antifungal Agents Antiviral Agents Beds COVID-19 COVID-19 pandemic COVID-19 patient COVID-19 treatment Centers for Disease Control and Prevention (U.S.)Cessation of life Chitosan Coagulants Copper Coronavirus Country Crustacea Disinfectants Disinfection Elevator Environment Environmental Protection Ethanol Feedback Film Food Additives Formulation Goals Growth Guidelines Hand Health Home Hospitals Hour Household Human Hydrogen Peroxide Hygiene Hypochlorite In Vitro Infection Manufactured Materials Measures Medical Microbiology Modeling Modernization Outcome Patients Performance Persons Polymers Population Prevention Preventive Property Protocols documentation Recommendation Resistance Resources Reverse Transcriptase Polymerase Chain Reaction Secondary to Services Silver Social Distance Solid Source South Carolina Surface Swab Technology Testing Textiles Thick Thinness Time Topical application Touch sensation Viral Viral Load result Viscosity Water anti-viral efficacy antimicrobial antimicrobial drug aqueous bactericide base disease transmission efficacy study experience experimental study health care settings human coronavirus member microbial novel novel coronavirus pandemic disease pathogen phase 1 study prevent prisma prototype respiratory transmission process virucide

项目摘要

Project Summary A novel coronavirus (SARS-CoV-2) has become one of the greatest challenges of the modern world and has caused a pandemic with millions of infected people and hundreds of thousands of deaths around the globe. Transmissions occur through respiratory droplets, as well as through contaminated hands and environmental surfaces. Therefore, cleaning and disinfection of potentially contaminated surfaces becomes one of the most important measures in prevention of coronavirus transmission, along with social distancing and hand hygiene. Current CDC guidelines for preventing transmission of coronavirus specifically recommend cleaning and disinfection of frequently touched surfaces. The use of commercially available disinfectants significantly reduces microbial counts on environmental surfaces for only up to 4 hours after treatment. An alcohol-based disinfectant with a compound capable of forming a durable but removable biocidal film could combine the advantages of both approaches described above. It would combine high short-term efficacy due to the presence of alcohol and prevent microbial re-contamination due to the long-lasting efficacy of the film, while requiring less frequent applications. VRM Labs has recently developed such disinfectant, which contains 3 wt. % chitosan and 60 % wt. ethanol. Chitosan is obtained from crustacean shells and its antimicrobial and antiviral efficacy is well-established. However, it has not been used in surface disinfectants because of high viscosity of its aqueous solutions and long drying times required to form a continuous film. Our proposed formulation has low viscosity, outstanding film-forming properties and drying time similar to that for currently used disinfectants. In preliminary studies, door handles treated by this novel formulation prevented microbial growth for up to 48 hours after treatment. The goal of this Phase I study is to create an optimized prototype of chitosan-based disinfectant and to test its bactericidal and viricidal performance in a limited-scale study in a healthcare setting. In Aim I, we will characterize film formation properties, including film thickness, film durability, abrasion resistance, and film drying times for formulations with different chitosan and ethanol concentrations. We will also evaluate in vitro antimicrobial and antiviral efficacy of these chitosan-based coatings with the most common pathogens involved known to be a source of infection on environmental surfaces, and with a surrogate coronavirus to mimic antiviral action against COVID-19. In Aim II, we will use the optimized formulation developed in Aim I to coat several designated elevator buttons, door handles, and bed rails in a medical facility (Greenville Memorial Hospital in South Carolina). We will then collect environmental swabs from these coated surfaces on a regular basis and compare the microbial and viral counts to those obtained from similar surfaces treated according to the current hospital protocol. These experiments will help to determine the efficacy time window for the coating.

项目摘要一项新颖的冠状病毒（SARS-COV-2）已成为现代世界的最大挑战之一，并已成为引起了大流行，全球数百万人和数十万人死亡。通过呼吸液滴以及受污染的手和环境进行传播表面。因此，对潜在受污染的表面的清洁和消毒成为最多的预防冠状病毒传播的重要措施，以及社会疏远和手动卫生。最新的CDC指南防止冠状病毒的传播专门建议清洁和对经常触摸的表面进行消毒。使用市售的消毒剂可显着降低环境表面的微生物计数治疗后最多只有4个小时。基于酒精的消毒剂，具有能够形成A的化合物耐用但可移动的杀生物膜可以结合上述两种方法的优势。会结合由于酒精的存在并防止因微生物重新污染而引起的高短期疗效膜的持久功效，同时需要频繁的应用。 VRM Labs最近开发了这种消毒剂，其中包含3 wt。％壳聚糖和60％wt。乙醇。壳聚糖是从甲壳类动物壳中获得的，其抗菌和抗病毒功效已良好。但是，由于其水溶液的高粘度和形成连续膜需要长的干燥时间。我们提出的配方具有低粘度，出色与当前使用的消毒剂相似的膜形成特性和干燥时间类似。在初步研究中，门通过这种新型配方处理的手柄可预防治疗后长达48小时的微生物生长。 I阶段我研究的目的是创建一个基于壳聚糖的消毒剂的优化原型，并测试其在医疗保健环境中的有限尺度研究中，杀菌性和疾病性能。在目标一世中，我们将描述膜形成特性，包括膜厚度，膜耐用性，耐磨性和膜干燥时间具有不同壳聚糖和乙醇浓度的配方。我们还将评估体外抗菌剂和这些基于壳聚糖的涂层的抗病毒功效，最常见的病原体涉及的是一种环境表面感染的来源，并具有替代冠状病毒，以模仿抗病毒作用新冠肺炎。在AIM II中，我们将使用在Aim I中开发的优化配方来覆盖几个指定的电梯在医疗机构（南卡罗来纳州的格林维尔纪念医院）的纽扣，门把手和床轨。我们然后，将定期从这些涂层表面收集环境拭子，并比较微生物和病毒计数是根据当前医院方案处理的类似表面获得的。这些实验将有助于确定涂层的功效时间窗口。