How Tetraspanins Regulate Sepsis

四跨膜蛋白如何调节脓毒症

基本信息

批准号：
10654676
负责人：
XIN A ZHANG
金额：
$ 29万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10654676
关键词：
Affect Animals Blood Cells Blood Vessels C-terminal Cause of Death Cell Adhesion Molecules Cell membrane Cysteine Cytoplasmic Tail Cytosol Disease E-Selectin Endothelial Cells Endothelium Endotoxins Event Exocytosis Extravasation Functional disorder Goals In Vitro Inflammation Inflammatory Inflammatory Response Injury KAI1 gene Knockout Mice Knowledge Leukocytes Lipopolysaccharides Mediating Membrane Molecular Molecular Target Morbidity - disease rate Multivesicular Body Organism Patients Proteins Recovery Role Sepsis Signaling Molecule Sorting Stimulus Structure Surface TNF gene Testing Thrombosis Transmembrane Domain Vascular Cell Adhesion Molecule-1 Vascular Diseases Vesicle animal mortality cadherin 5 cytokine disulfide bond exosome extracellular glycosylation improved in vivo insight mortality new therapeutic target palmitoylation rab GTP-Binding Proteins recruit response septic septic patients trafficking

项目摘要

Vascular inflammatory responses determine the onset, progression, and consequence of sepsis. Tetraspanin CD82 proteins are expressed in endothelial cells (ECs) and leukocytes. Our recent study revealed that CD82 facilitates vascular inflammatory responses during sepsis. We demonstrated that, to facilitate inflammation, endothelial CD82 promotes lipopolysaccharide (LPS)- induced vascular leakage, leukocyte recruitment, and endothelial release of cytokines, leading to marked increase in mortality of animal with sepsis. How CD82 promotes the inflammatory events in blood vessels during sepsis is unclear. The goal of this study is to identify the mechanisms by which CD82 facilitates vascular inflammatory responses in sepsis at the molecular, cellular, and organism levels. We hypothesize that, CD82 facilitates the vesicular trafficking of inflammation-related molecules such as VE- cadherin, E-selectin, and cytokines, to promote inflammatory responses in sepsis and therefore exacerbate sepsis. In this project, we will first determine how CD82 inhibits vascular stability in sepsis by assessing the regulatory roles of CD82 in i) inflammation-induced disruption of EC-EC contacts/junctions and ii) recovery of endothelial barriers from the disrupted EC-EC contacts/junctions. Secondly, we will determine the mechanism by which CD82 promotes vascular recruitment of leukocytes during sepsis by assessing CD82 effects on the i) levels, ii) activities, and iii) turnover of inflammation- related cell adhesion proteins in ECs and leukocytes during sepsis. Finally, we will determine how CD82 inhibits cytokine secretion in sepsis by i) assessing in vivo and in vitro effects of CD82 on cytokine secretomes, ii) revealing CD82 roles in exosome formation and exocytosis, and iii) identifying the Rab GTPase(s) crucial for CD82-regulated exocytosis. This project will fill important knowledge gaps for the regulatory mechanisms of vascular inflammatory events in response to septic injury, leading to systemic vascular dysfunction, at the molecular, cellular, and organism levels. This project will also establish CD82 as a novel therapeutic target for ameliorating systemic vascular dysfunction during sepsis and improving the survival of sepsis patients.

血管炎症反应决定炎症的发生、进展和后果败血症。四跨膜蛋白 CD82 蛋白在内皮细胞 (EC) 和白细胞中表达。我们的最近的研究表明 CD82 促进脓毒症期间的血管炎症反应。我们证明，为了促进炎症，内皮 CD82 促进脂多糖 (LPS)- 诱导血管渗漏、白细胞募集和内皮细胞因子释放，从而导致患有败血症的动物死亡率显着增加。 CD82如何促进炎症事件脓毒症期间的血管尚不清楚。本研究的目的是确定 CD82 促进血管生成的机制。脓毒症在分子、细胞和生物体水平上的炎症反应。我们假设 CD82 促进炎症相关分子（例如 VE-）的囊泡运输钙粘蛋白、E-选择素和细胞因子，促进脓毒症中的炎症反应，因此加剧败血症。在这个项目中，我们将首先通过评估确定 CD82 如何抑制脓毒症中的血管稳定性 CD82 在 i) 炎症诱导的 EC-EC 接触/连接破坏中的调节作用，以及 ii)从破坏的EC-EC接触/连接处恢复内皮屏障。其次，我们将确定 CD82 促进白细胞血管募集的机制通过评估 CD82 对 i) 水平、ii) 活动和 iii) 炎症周转的影响来评估脓毒症- 脓毒症期间 EC 和白细胞中的相关细胞粘附蛋白。最后，我们将确定如何 CD82 通过 i) 评估 CD82 对脓毒症的体内和体外作用来抑制细胞因子分泌细胞因子分泌组，ii) 揭示 CD82 在外泌体形成和胞吐作用中的作用，以及 iii) 鉴定对 CD82 调节的胞吐作用至关重要的 Rab GTP 酶。该项目将填补血管调节机制的重要知识空白脓毒性损伤引起的炎症事件，导致全身血管功能障碍分子、细胞和有机体水平。该项目还将把CD82打造成一部小说改善脓毒症期间全身血管功能障碍的治疗目标脓毒症患者的生存率。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

EWI2 promotes endolysosome-mediated turnover of growth factor receptors and integrins to suppress lung cancer.

DOI：
10.1016/j.canlet.2022.215641
发表时间：
2022-06-28
期刊：
CANCER LETTERS
影响因子：
9.7
作者：
Wang, Jie;Wren, Jonathan D.;Ding, Yingjun;Chen, Junxiong;Mittal, Nikhil;Xu, Chao;Li, Xing;Zeng, Cengxi;Wang, Meng;Shi, Jing;Zhang, Yanhui H.;Han, Sangyoon J.;Zhang, Xin A.
通讯作者：
Zhang, Xin A.

A Bioinformatics Perspective on the Links Between Tetraspanin-Enriched Microdomains and Cardiovascular Pathophysiology.