A COMPREHENSIVE RESOURCE BASE OF C. ELEGANS K+ CHANNELS

线虫 K 通道的综合资源库

• 批准号: 6751713
• 负责人: LAWRENCE B SALKOFF
• 金额: $ 42.41万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-23 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6751713
• 关键词: Caenorhabditis elegans; Xenopus; Xenopus oocyte; animal genetic material tag; complementary DNA; genetic registry /resource /referral center; genetically modified animals; helminth genetics; potassium channel; transfection /expression vector; voltage /patch clamp

DESCRIPTION (provided by applicant): The C. elegans genome contains an extended gene family of potassium channels consisting of more than seventy members. Among these genes are an ancient conserved "set" of potassium channels with orthologues in both humans and Drosophila. Many of these conserved genes are of interest to researchers working in fields as diverse as cardiac physiology, neurological function, and kidney and digestive disorders. Thus, C. elegans has the potential of being a valuable model system for basic research in virtually all fields where excitable membranes and ion currents are important. The investigators propose to create a resource base that will include full-length potassium channel cDNA clones in vectors suitable for functional expression in mammalian cell lines and Xenopus oocytes, and stocks of gene KO strains. Supporting this resource base will be studies showing the basic biophysical properties of the channels, studies showing the phenotypes of KO mutants and information on the cell-type expression patterns of each channel. Many aspects of this comprehensive project will directly guide studies in mammals and should offer an alternative model system applicable to a wide variety of researchers in both basic and applied research.

描述（由申请人提供）： 秀丽隐杆线虫基因组包含钾通道的扩展基因家族，由七十多个成员组成。 这些基因中有一组古老的保守钾通道，在人类和果蝇中都有直系同源物。 许多保守基因引起了心脏生理学、神经功能、肾脏和消化系统疾病等不同领域的研究人员的兴趣。 因此，线虫有潜力成为一个有价值的模型系统，可用于几乎所有可兴奋膜和离子流很重要的领域的基础研究。 研究人员提议创建一个资源库，其中包括适合在哺乳动物细胞系和非洲爪蟾卵母细胞中功能表达的载体中的全长钾通道 cDNA 克隆，以及基因 KO 菌株的库存。 支持该资源库的研究将显示通道的基本生物物理特性、显示 KO 突变体表型的研究以及有关每个通道的细胞类型表达模式的信息。 这个综合项目的许多方面将直接指导哺乳动物的研究，并应提供适用于基础研究和应用研究的各种研究人员的替代模型系统。