High-resolution functional imaging of the retina

视网膜的高分辨率功能成像

基本信息

批准号：
9566002
负责人：
Jennifer J Hunter
金额：
$ 33.57万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-04-01 至 2020-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9566002
关键词：
Aerobic Affect All-Trans-Retinol Anaerobic Bacteria Anterior Apoptosis Biochemical Process Biological Markers Cell Death Cell Respiration Cell physiology Cells Characteristics Clinical Dark Adaptation Diagnosis Disease Disease Progression Early Diagnosis Early identification Equilibrium Eye Fluorescence Functional Imaging Functional disorder Funding Future Generations Glaucoma Health Human Hypoxia Image Individual Injury Intervention Laboratory Animals Lead Leber&apos s Hereditary Optic Neuropathy Light Macaca Macular degeneration Measurement Measures Metabolic Metabolism Methods Monitor Monkeys Mus NADH Nature Normal Range Ophthalmoscopes Ophthalmoscopy Optics Oxidation-Reduction Pathologic Pathology Pharmacology Photic Stimulation Photons Photoreceptors Physiological Physiology Primates Process Production Property Research Resolution Retina Retinal Retinal Cone Retinal Degeneration Retinal Diseases Retinal Ganglion Cells Retinoids Rodent Scanning Spectrum Analysis Time Translations Treatment Efficacy Variant Vision absorption adaptive optics calcium indicator experimental study fluorescence imaging fluorophore ganglion cell human disease improved in vivo insight method development molecular dynamics non-invasive imaging novel optical spectra response retinal imaging retinal rods two-photon visual cycle

项目摘要

Project Summary To understand visual function and cellular metabolism in the living eye, we need a means to visualize individual cells and assess molecular dynamics non-invasively. Some of the key molecules involved in the visual cycle and cellular energy production are intrinsically fluorescent, but are inaccessible in the living eye through single-photon excitation because the needed wavelength range is not transmitted by the optics of the eye. Two-photon excited fluorescence ophthalmoscopy (TPEFO) can excite these otherwise inaccessible fluorophores with near-infrared light. In our previous experiments in the living macaque eye, we used an adaptive optics scanning light ophthalmoscope (AOSLO) to non-invasively image many different cell classes throughout the retina, including ganglion cells (Sharma, Williams et al., 2016). Additionally, we measured visual cycle function by characterizing the time course of two-photon excited fluorescence from photoreceptors (Sharma, Schwarz et al., 2016). We now propose to expand the capabilities of TPEFO in the living rodent and macaque eye to measure several important fluorescence properties, such as emission spectrum, fluorescence lifetime, and redox ratio, which may be indicators of cell health and function. This research has the potential to provide insight into normal and altered biochemical processes and improve our understanding of diseases that impact retinal metabolism and function such as glaucoma, macular degeneration and Leber hereditary optic neuropathy.

项目概要为了了解活体眼睛的视觉功能和细胞代谢，我们需要一种可视化的方法单个细胞并非侵入性地评估分子动力学。涉及的一些关键分子视觉循环和细胞能量产生本质上是荧光的，但在通过单光子激发活体眼睛，因为所需的波长范围不是通过眼睛的光学系统传输。双光子激发荧光检眼镜 (TPEFO) 用近红外光激发这些原本难以接近的荧光团。在我们之前的实验中在活体猕猴的眼睛中，我们使用自适应光学扫描光检眼镜（AOSLO）对整个视网膜中许多不同的细胞类别进行非侵入性成像，包括神经节细胞（夏尔马、威廉姆斯等人，2016）。此外，我们通过表征视觉周期功能来测量光感受器双光子激发荧光的时间过程（Sharma、Schwarz 等人，2016）。我们现在建议将 TPEFO 在活体啮齿动物和猕猴眼睛中的能力扩展到测量几个重要的荧光特性，例如发射光谱、荧光寿命、和氧化还原比，这可能是细胞健康和功能的指标。这项研究有潜力提供对正常和改变的生化过程的洞察，并提高我们对影响视网膜代谢和功能的疾病，如青光眼、黄斑变性和莱伯遗传性视神经病。