Core B - Molecular Pathology and Imaging Core (MPIC)

核心 B - 分子病理学和成像核心 (MPIC)

• 批准号: 10214548
• 负责人: HANINA HIBSHOOSH
• 金额: $ 32.32万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10214548
• 关键词: 3-Dimensional; Address; Animals; Bass; Biomedical Research; Biometry; Biostatistics Core; Cancer Family; Cell Line; Cells; Clinical; Core Facility; Country; Cultured Cells; Dana-Farber Cancer Institute; Development; Digestive System Disorders; Emerging Technologies; Ensure; Equipment; Esophageal Squamous Cell; Esophagus; Experimental Designs; Exposure to; Floor; Fostering; Goals; Grant; Histopathology; Human; Human Resources; Image; Image Analysis; Immunofluorescence Immunologic; Immunohistochemistry; Immunooncology; In Situ Hybridization; Individual; Institutes; Laboratories; Laboratory Personnel; Liver diseases; Location; Malignant neoplasm of esophagus; Medical; Microscope; Modeling; Molecular; Mus; New York; Organ; Organoids; Pathologic; Pathologist; Pathology; Pennsylvania; Preparation; Productivity; Protocols documentation; Reagent; Reproducibility; Research; Research Institute; Research Personnel; Research Training; Resources; Schools; Services; Site; Slide; South Carolina; Specialist; Stains; System; Techniques; Technology; Tissues; Training; Universities; Visualization; cohesion; cost effective; design; esophageal carcinogenesis; experience; experimental study; imaging capabilities; imaging system; instrument; liquid crystal polymer; live cell imaging; medical schools; meetings; member; microscopic imaging; molecular pathology; multiplexed imaging; new technology; pathology imaging; programs; square foot; tissue processing

PROJECT SUMMARY – CORE B The Molecular Pathology and Imaging Core (MPIC), Core B, is strategically designed to address the shared needs for tissue processing, histopathology, imaging, and expert analyses and is integral to all three projects of this P01. Established in 1995, the MPIC has been a critical part of this P01 since its inception in 2003 and has served as a model core facility for numerous other program projects and centers at the University of Pennsylvania and throughout the country. The overarching goal of the MPIC is to provide exceptional service and support to the esophageal cancer P01 investigators and their laboratories and is accomplished through two interrelated Specific Aims: (1) To provide essential, cost-effective, and high-quality services and support to the P01 investigators and their laboratories for the preparation, sectioning, staining, and interpretation of tissue sections, both animal and human, as well as cultured cells (interacting with CCiC), with particular expertise and focus on 3D organotypic/organoid cultures; and (2) To assist in planning and optimizing (a) in situ hybridization (ISH), immunohistochemistry (IHC), and immunofluorescence (IF) experiments, including for immuno-oncology, and (b) microscopy and image analyses for the P01 members and their laboratories and to provide training in these techniques, as well as perform these services as requested. The MPIC is led by an experienced Director (Dr. Jonathan Katz), Technical Director (Mr. Adam Bedenbaugh), and expert Consultant in esophageal and squamous cell pathology (mouse and human) (Dr. Andres Klein-Szanto). Integrating resources and services across all project sites, the MPIC is a unique entity at the University of Pennsylvania, the Medical University of South Carolina, the Dana Farber Cancer Institute and New York Institute with specific expertise in processing and analyses of all 3D organotypic cultures/organoid cultures and tissues (mouse, human) relevant to this P01. For P01 investigators and their laboratory personnel, the MPIC provides both essential, routine services and advanced technologies and approaches. The MPIC also fosters cost-effective approaches and promotes new and exciting directions for research. In addition, the MPIC offers special benefits to members of this P01 that are not available to other users, including esophageal cancer specific TMAs, significant subsidies for services, priority services, and access to Dr. Klein-Szanto, an expert clinical pathologist. Moreover, through consistent and validated approaches and standard protocols, the MPIC helps ensure rigor and reproducibility of experiments for P01 investigators and their laboratory members. Thus, the MPIC provides exceptional utility, quality, value, experience, service, and commitment to this P01's projects, cohesive coordination with other cores, and this P01 receives top priority.

项目摘要 - 核心B 分子病理学和成像核（MPIC），核心B，旨在解决共享 组织处理，组织病理学，成像和专家分析的需求，并且是所有三个项目的组成部分 这个P01。 MPIC成立于1995年，自2003年成立以来一直是该P01的关键部分 曾在大学的许多其他计划项目和中心充当模型核心设施 宾夕法尼亚州和全国。 MPIC的总体目标是提供卓越的服务 并支持食管癌P01研究者及其实验室，并通过 两个相互关联的具体目的：（1）提供基本，成本效益和高质量的服务和支持 向P01调查人员及其实验室进行准备，切片，染色和解释 动物和人类的组织切片以及培养的细胞（与CCIC相互作用），特别是 专业知识和专注于3D器官/器官培养物； （2）协助计划和优化（a） 原位杂交（ISH），免疫组织化学（IHC）和免疫荧光（IF）实验，包括用于 免疫肿瘤学，（b）P01成员及其实验室的显微镜和图像分析以及 提供这些技术的培训，并根据要求提供这些服务。 MPIC由 经验丰富的董事（Jonathan Katz博士），技术总监（Adam Bedenbaugh先生）和专家顾问 整合在食管和鳞状细胞病理学（小鼠和人）（Andres Klein-Szanto博士）中。 MPIC的所有项目网站的资源和服务是宾夕法尼亚大学的独特实体， 南卡罗来纳州医科大学，达娜·法伯癌症研究所和纽约学院 在所有3D器官培养物/器官培养和组织的处理和分析方面的专业知识（小鼠， 与此P01相关的人）。对于P01调查人员及其实验室人员，MPIC都提供 必不可少的常规服务以及高级技术和方法。 MPIC还促进了成本效益 接近并促进了新的和令人兴奋的研究方向。此外，MPIC提供特别 对于其他用户（包括食管癌），该P01成员的好处 TMA，服务，优先服务的大量补贴以及专家临床Klein-Szanto博士的访问权限 病理学家。此外，通过一致且经过验证的方法和标准协议，MPIC帮助 确保P01研究人员及其实验室成员实验的严格性和可重复性。那， MPIC为P01的项目提供了卓越的实用性，质量，价值，经验，服务和承诺， 与其他核心的凝聚协调性，该P01获得了最高优先级。