Bio-electrochemical detectors for in vivo continuous monitoring
用于体内连续监测的生物电化学检测器
基本信息
- 批准号:9238429
- 负责人:
- 金额:$ 57.31万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-01 至 2021-06-30
- 项目状态:已结题
- 来源:
- 关键词:AffinityAmino AcidsAminoglycoside AntibioticsAminoglycosidesAnimal ModelAnticoagulationBiological MarkersBiomedical ResearchBiosensorBladderBloodBrainCaliberChemistryCircadian RhythmsClinicalCoagulation ProcessComplexDNADetectionDevelopmentDiseaseDoseDoxorubicinDrug ControlsDrug Delivery SystemsDrug KineticsDrug TargetingFeedbackFutureGoalsHalf-LifeHealthHealth StatusHealthcareHourIn VitroIndividualIntercellular FluidKanamycinKnowledgeLaboratoriesLaboratory AnimalsMeasurementMeasuresMembraneMetabolicMetabolismMethodsMolecularMolecular TargetMonitorOryctolagus cuniculusOutcomePatientsPharmaceutical PreparationsPhysiologicalPhysiologyRattusReporterResearchResolutionRunningSamplingSensitivity and SpecificitySpecificityTechnologyTestingTherapeuticTherapeutic IndexTimeTissuesTobramycinTranslatingUrineValidationVariantVeinsWhole Bloodaptamerawakebasebiomaterial compatibilitybrain tissueclinical applicationclinical practiceclinically relevantdetectorimprovedin vivonovel therapeuticsoperationpre-clinicalprogramssensorspecific biomarkersstability testingtemporal measurementtool
项目摘要
Summary. Our goal is the development and validation of a technology that can measure a wide range of drugs,
metabolites, and biomarkers in the blood, urine, and interstitial fluids of living subjects continuously and in real
time, an ability that would revolutionize many aspects of biomedical research and healthcare. To achieve this
goal we will combine electrochemical aptamer-based sensors (which are reagentless and wash-free and can
operate directly in complex samples without fouling) with biocompatible anti-coagulation membranes and
advanced drift correction methods to achieve continuous, real-time, multi-day measurements directly in vivo.
As preliminary results supporting this goal we demonstrate the continuous, real-time measurement of four
different drugs directly in the jugulars of anesthetized rats for periods of more than five hours with 20-second
time resolution and sub-micromolar precision. Leveraging these initial results we propose here three further
advancements: First, we will expand the platform to the in vivo measurement of nine additional drugs and
metabolites, which will demonstrate the platform's versatility. Second, we will improve the platform's stability
such that we can perform high-precision in vivo measurements over the course of days, which will enable
continuous measurements of long-lived drugs and capture clinically important circadian metabolic variations.
Finally, we will expand the platform to the simultaneous measurement of multiple molecules and/or across
multiple body compartments (e.g., blood, tissues, brain, bladder), rendering it a powerful new tool for
understanding pharmacokinetics and physiology. By creating an unprecedented window into a patient's
molecular-level physiological state, the proposed technology would enable many transformative clinical
applications, including the high-precision measurement of patient-specific pharmacokinetics, the continuous
monitoring of health status via the real-time measurement of specific biomarkers, and, ultimately, feedback-
controlled drug delivery.
概括。我们的目标是对可以测量广泛药物的技术的开发和验证,
活性受试者的血液,尿液和间质性流体中的代谢物以及生物标志物不断,实际上是
时间,这种能力将彻底改变生物医学研究和医疗保健的许多方面。实现这一目标
目标我们将结合基于电化学适体的传感器(无试剂无洗,可以
直接在复杂的样品中运行而无需结垢),并用生物相容性的抗凝结膜和
直接在体内直接实现连续的,实时的多天测量的高级漂移校正方法。
作为支持此目标的初步结果,我们证明了四个的连续实时测量
直接在麻醉大鼠的颈颈中的不同药物超过五个小时,有20秒
时间分辨率和亚微摩尔精度。利用这些初始结果,我们在这里提出了三个
进步:首先,我们将将平台扩展到体内测量九种其他药物和
代谢物将展示该平台的多功能性。其次,我们将改善平台的稳定性
这样我们就可以在几天内执行高精度在体内测量结果,这将使
长寿命药物的连续测量并捕获临床上重要的昼夜节律代谢变异。
最后,我们将将平台扩展到多个分子和/或跨度的同时测量
多个身体室(例如,血液,组织,大脑,膀胱),使其成为一个强大的新工具
了解药代动力学和生理学。通过创建一个前所未有的窗口到患者的
分子水平的生理状态,提出的技术将使许多变革性临床
应用,包括对患者特异性药代动力学的高精度测量,连续的
通过实时测量特定的生物标志物来监测健康状况,并最终反馈 -
受控药物输送。
项目成果
期刊论文数量(0)
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Kevin W Plaxco其他文献
Kevin W Plaxco的其他文献
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{{ truncateString('Kevin W Plaxco', 18)}}的其他基金
Biostable nucleic acid aptamers for long-duration, in vivo molecular monitoring
用于长时间体内分子监测的生物稳定核酸适体
- 批准号:
10304801 - 财政年份:2021
- 资助金额:
$ 57.31万 - 项目类别:
Biostable nucleic acid aptamers for long-duration, in vivo molecular monitoring
用于长时间体内分子监测的生物稳定核酸适体
- 批准号:
10430240 - 财政年份:2021
- 资助金额:
$ 57.31万 - 项目类别:
Feedback controlled, ultra-high-precision drug delivery
反馈控制的超高精度药物输送
- 批准号:
10084266 - 财政年份:2019
- 资助金额:
$ 57.31万 - 项目类别:
Feedback controlled, ultra-high-precision drug delivery
反馈控制的超高精度药物输送
- 批准号:
10321612 - 财政年份:2019
- 资助金额:
$ 57.31万 - 项目类别:
Feedback controlled, ultra-high-precision drug delivery
反馈控制的超高精度药物输送
- 批准号:
9761770 - 财政年份:2019
- 资助金额:
$ 57.31万 - 项目类别:
Bio-electrochemical detectors for in vivo continuous monitoring
用于体内连续监测的生物电化学检测器
- 批准号:
9551624 - 财政年份:2017
- 资助金额:
$ 57.31万 - 项目类别:
A new approach to quantitative, point-of-care serology
定量、护理点血清学的新方法
- 批准号:
9306748 - 财政年份:2014
- 资助金额:
$ 57.31万 - 项目类别:
A new tool for measuring surface-biomolecule interactions
测量表面生物分子相互作用的新工具
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8662567 - 财政年份:2014
- 资助金额:
$ 57.31万 - 项目类别:
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