Conformational change in NSS transporters

NSS转运蛋白的构象变化

基本信息

批准号：
9507971
负责人：
GARY W RUDNICK
金额：
$ 55.08万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-06-15 至 2021-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9507971
关键词：
Affinity Amines Amino Acid Transporter Amino Acids Animals Binding Binding Sites Biochemical Biological Biophysics Brain Carrier Proteins Cell membrane Cells Coupled Couples Coupling Crystallization Cysteine Diet Disease Drosophila genus Family Family member Glycine Goals Health Human Intestines Investigation Iodides Ion Cotransport Ions Kidney Ligand Binding Ligands Link Measurement Mediating Membrane Membrane Transport Proteins Minerals Molecular Conformation Molecular Structure Monitor Movement Mutation Nature Neurons Neurotransmitters Occupations Pathway interactions Peripheral Physiological Processes Process Prokaryotic Cells Proteins Pump Resolution Role Sampling Serotonin Side Site Sodium Structure Sugar Acids Testing Thyroid Gland Transport Process Work antiport base conformational conversion design driving force glycine transporter inhibitor/antagonist insight molecular dynamics monoamine mutant neurotransmitter reuptake novel nutrient absorption protein folding protein structure protein transport radioligand response serotonin transporter single-molecule FRET small molecule solute symporter uptake

项目摘要

Abstract Our goal is to understand the mechanism by which a large family of transport proteins moves small molecules and ions across biological membranes. In recent years it has become apparent that many families of transport proteins are related at the structural level. The transporter proteins are responsible for moving a wide variety of substrate molecules across cell membranes. They mediate neurotransmitter re-uptake in the brain, sugar and amino acid transport in kidney and intestine, iodide transport in thyroid, and many other important transport processes that are critically important in health and disease. These transporter families are all based on a protein fold containing two structurally similar repeats in opposite orientations relative to the membrane. Recent structural evidence indicates that transport requires a conformational change in which a 4-helix bundle rearranges within the protein structure. However, the ability of these transporters to accumulate substrates within the cell using the coupled movement of ions is a separate issue not addressed by previous studies. The specific aims of this project are to use biochemical, biophysical and computational approaches to test proposed mechanisms, and to understand how binding of substrate and ions can trigger conformational changes relating to the movement of the bundle and the consequent opening and closing of permeation pathways.

抽象的我们的目标是了解转运蛋白大家族的移动机制小分子和离子穿过生物膜。近年来已经很明显许多转运蛋白家族在结构水平上是相关的。运输者蛋白质负责在细胞间移动多种底物分子膜。它们介导大脑中神经递质、糖和氨基酸的重新摄取肾脏和肠道的运输、甲状腺中的碘化物运输以及许多其他重要的对健康和疾病至关重要的运输过程。这些运输机家族都基于包含两个结构相似的重复序列的蛋白质折叠相对于膜的方向。最近的结构性证据表明，运输需要构象变化，其中 4 螺旋束在蛋白质内重新排列结构。然而，这些转运蛋白在细胞内积累底物的能力使用离子的耦合运动是一个单独的问题，以前的研究没有解决。这该项目的具体目标是使用生物化学、生物物理和计算方法测试所提出的机制，并了解底物和离子的结合如何触发与束的运动和随后的打开相关的构象变化和关闭渗透通道。