Mechanisms of SPW-R sequencing and termination

SPW-R 测序和终止机制

• 批准号: 9442584
• 负责人: GYORGY BUZSAKI
• 金额: $ 26.05万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9442584
• 关键词: Apical; Biological Neural Networks; Data; Electrophysiology (science); Event; Hippocampus (Brain); Image; Interneurons; Light; Measures; Medial; Mediating; Memory; Methods; Neurosciences; Optical Methods; Optics; Pattern; Play; Positioning Attribute; Probability; Process; Pyramidal Cells; Recruitment Activity; Role; Signal Transduction; Silicon; Site; Source; Testing; cell type; density; dentate gyrus; experimental study; extracellular; independent component analysis; operation; optogenetics; simulation; two-photon; voltage

The memory function of SPW-Rs depends critically on rapid sequential replay of memory items (e.g. spatial positions along an explored path). The question of how neural networks can produce accurate sequencing is an unsolved problem in neuroscience. Whereas Project 1 deals with the question of how SPW-Rs are initiated, Project 2 deals with how memory sequences are produced. Critical to this project is the need to determine the firing patterns of pyramidal cells and each of the major interneuron types during SPW-Rs. A large number of powerful new optical methods will be used to obtain this data. A second class of experiments will use optogenetic inactivation methods to determine whether the sequencing operation can be produced by events in CA2/CA3 alone, or whether the dentate gyrus (DG) can also influence SPW-R content. We further hope to reveal the basis of important discretization processes that govern SPW-R-related memory sequencing. There have been indications that memory readout during SPW-Rs is discretized by gamma oscillations - in Project 2, we will attempt to verify this and shed light on the basis of this timing operation. Other experiments will provide information relevant to understanding SPW-R termination processes. Collectively, this information, in conjunction with the analysis and simulations proposed in Project 5, is likely to answer fundamental questions about the cellular and network mechanisms governing the recruitment and sequencing of memory content during SPW-R-related replay.

SPW-RS的内存功能严重取决于记忆项目的快速顺序重播 （例如，沿探索路径的空间位置）。神经网络如何产生的问题 准确的测序是神经科学中未解决的问题。而项目1则处理 SPW-RS如何启动的问题，项目2涉及如何产生内存序列。 这个项目至关重要的是需要确定锥体细胞的发射模式和每个 SPW-RS期间主要的中间神经元类型。大量强大的新光学方法将是 用于获取此数据。第二类实验将使用光学遗传灭活方法 确定是否仅在CA2/CA3中进行的事件可以产生测序操作，或者 齿状回（DG）是否也会影响SPW-R含量。我们进一步希望揭示 支持SPW-R相关记忆测序的重要离散过程的基础。那里 迹象表明，SPW -RS期间的内存读数已被γ振荡离散 - 在项目2中，我们将尝试根据此计时操作来验证这一点并阐明。其他 实验将提供与了解SPW-R终止过程有关的信息。 总的来说，这些信息以及项目中提出的分析和模拟 5，很可能会回答有关控制细胞和网络机制的基本问题 在SPW-R相关重播过程中，记忆内容的募集和测序。