ShEEP Request for Keyence BZ-X800E All-in-One Automated Imaging System

ShEEP 请求 Keyence BZ-X800E 一体化自动化成像系统

• 批准号: 9793454
• 负责人: TIBOR KRISTIAN
• 金额: --
• 依托单位: BALTIMORE VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9793454
• 关键词: 3-Dimensional; Agonist; Antibodies; Area; Baltimore; Bioenergetics; Brain; Brain Injuries; Brain Ischemia; Brain-Derived Neurotrophic Factor; Cell Count; Cell Death; Chronic; Clinical Sciences; Colon Carcinoma; Color; Comb animal structure; Computer software; Contracts; Development; Dimensions; Doctor of Medicine; Doctor of Philosophy; Encephalomyelitis; Equipment; Event; Exposure to; Failure; Fluorescence; Funding; General Population; Goals; Head; Health; Histologic; Homing; Human; Hybrid Cells; Image; Imagery; Immunity; Immunohistochemistry; Incubators; Inflammation; Injury; Intuition; Ischemia; Ischemic Brain Injury; Laboratory Research; Lead; Macrophage Activation; Maintenance; Matrilysin; Measurement; Medical center; Mental disorders; Methods; Microglia; Microscope; Mitochondria; Modeling; Monitor; Monoclonal Antibodies; Multiple Sclerosis; Oils; Operative Surgical Procedures; Organ; Organelles; Pattern; Pharmacotherapy; Phase; Price; Principal Investigator; Process; Program Evaluation; Rehabilitation Research; Reperfusion Injury; Reperfusion Therapy; Request for Proposals; Research; Research Personnel; Resolution; Risk; Scanning; Series; Services; Shapes; Shipping; Signal Transduction; Soldier; Specimen; Spinal Cord; Stress; Stroke; System; Testing; Texas red; Thick; Three-dimensional analysis; Time; Tissues; Training; Translational Research; Traumatic Brain Injury; Veterans; Work; cancer cell; chemokine receptor; colon cancer progression; contrast imaging; cost; cyanine dye 5; design; discount; fluorescence imaging; fluorescence microscope; high resolution imaging; imaging system; improved; innovation; instrument; interest; lens; live cell imaging; medical specialties; mouse model; neuroinflammation; novel; novel therapeutics; processing speed; research and development; stroke victims; targeted treatment; three dimensional structure; tissue processing; tool; translational study; traumatic event

The objective of this Shared Equipment Evaluation Program (ShEEP) proposal is to request Keyence BZX800E All-in-One Automated Imaging System which will offer researchers an instrument that is capable of: confocal-like multi-stack imaging and real-time focusing of thick tissue sections; rapid automatic high- magnification image acquisition and stitching of composite images; and three-dimensional profiling of serial tissue sections imaged at high resolution. The control software is simple and intuitive. Thus, with minimal training a novice user can scan a series of tissue sections from an entire organ, generate high-resolution composites of each tissue section, and merge all composites into a single three-dimensional rendering of the entire tissue. Traditional microscopes available at the Baltimore VA Medical Center (BVAMC) do not possess these capabilities. The VA projects cover an array of specialties as described below. Each will benefit from Keyence BZX800E All-in- One Automated Imaging System. The BZX800E Microscope optimizes the imaging process by designing a fully automated, easy-to-use system that captures high-resolution images and allows researchers to achieve their desired results faster. These applications are of great use for VA Biomedical Laboratory Research and Development Service (BLR&D), Clinical Science Research and Development (CSR&D), and Rehabilitation Research and Development (RR&D) funded investigators. Tibor Kristian, Ph.D. will serve as the Project Director (PD)/ Principal Investigator (PI) for this proposal. Dr. Kristian’s study focuses on understanding injury mechanisms that lead to mitochondrial and ultimately cellular bioenergetic failure in stroke victims. This imaging system is essential for imaging and quantification of intracellular organelles damage following stroke- induced ischemic brain injury. Bingren Hu, M.D., Ph.D. is testing a novel hypothesis that brain ischemia- reperfusion leads to a cascade of events resulting in inactivation; as well as study novel mechanisms underlying brain ischemia-reperfusion (IR) injury. Many antibodies will be used in Aims 1 and 2 of Dr. Hu’s studies; and the BZX800 microscope is necessary for imaging and quantification of intracellular organelles damage following IR brain injury. Bogdan Stoica, M.D. will use this new system to investigate traumatic brain injury (TBI). Dr. Stoica’s project requires significant imaging of chronic neuroinflammation markers and secondary damaging markers. Tapas Makar, Ph.D.’s project studies the effects of 7, 8- dihydroxyflavone (7,8- DHF); which has potent brain derived neurotrophic factor (BDNF) agonist activity and will use the imaging system in his specific aims 2 and 3. Dr. Makar will determine whether DHF treatment modulates inflammation and immunity in the brain and spinal cord in encephalomyelitis (EAE), a murine model of Multiple sclerosis, using monoclonal antibodies. Leonardo Tonelli, Ph.D.’s project studies the stress of deployment and exposure to traumatic events that puts soldiers at a greater risk than the general public in developing psychological disorders. Dr. Tonelli’s analysis will consist of immunohistochemistry in adjacent sections for: homing markers, chemokine receptors, microglia and macrophage activation and/or differentiation- all of which can be viewed with the Keyence BZ-X800. Guofeng Xie, M.D. studies colon cancer and his long-term goal is to develop innovative, effective, and safe therapies by targeting pivotal signal transduction molecules underlying colon cancer progression. The microscope will allow Dr. Xie to examine the expression patterns of matrix metalloproteinase 7 (MMP7) using immunofluorescent anti-MMP7 antibody in human colon cancer cells and surgical specimens. The funded projects form the basis for translational studies that will improve treatment in Veterans- potentially yielding new therapeutics.

该共享设备评估计划（Sheep）建议的目的是请求密钥 BZX800E多合一的自动成像系统，它将为研究人员提供一种能够： 厚组织切片的共聚焦多堆栈成像和实时聚焦；快速自动高 - 复合图像的放大图像采集和缝合；和串行的三维分析 以高分辨率成像的组织切片。控制软件简单明了。那很少 训练新颖的用户可以从整个器官扫描一系列的组织切片，产生高分辨率 每个组织部分的复合材料，并将所有共子材料合并成单个三维渲染 整个组织。巴尔的摩VA医疗中心（BVAMC）可用的传统显微镜不具备 这些功能。 如下所述，VA项目涵盖了一系列专业。每个都将从钥匙bzx800e中受益 一个自动成像系统。 BZX800E显微镜通过 设计一个完全自动化，易于使用的系统，可捕获高分辨率图像并允许研究人员 更快地实现他们所需的结果。这些应用非常适合VA生物医学实验室 研发服务（BLR＆D），临床科学研发（CSR＆D），以及 康复研发（RR＆D）资助的研究人员。蒂博·克里斯蒂安（Tibor Kristian）博士将作为 该提案的项目总监（PD）/首席研究员（PI）。克里斯蒂安博士的研究重点 了解导致线粒体和最终细胞生物能衰竭的损伤机制 中风可怕。该成像系统对于细胞内细胞器的成像和量化至关重要 中风引起的缺血性脑损伤后的损伤。 Bingren Hu，医学博士，博士正在检验一个新的假设 大脑缺血 - 再灌注导致一系列级联，导致失活。以及研究小说 脑缺血再灌注（IR）损伤的机制。目标1和2将使用许多抗体 Hu博士的研究； BZX800显微镜对于对细胞内的成像和定量是必需的 红外脑损伤后细胞器损伤。博格丹·斯托卡（Bogdan Stoica），医学博士将使用这个新系统进行调查 创伤性脑损伤（TBI）。 Stoica博士的项目需要对慢性神经炎症进行大量成像 标记和次要破坏标记。 TAPAS MAKAR，博士的项目研究7、8-的效果 二羟基氟法酮（7,8-DHF）;具有潜在的大脑衍生神经营养因子（BDNF）激动剂活性和 将在其特定目标2和3中使用成像系统。Makar博士将确定DHF治疗是否治疗 调节脑脊髓炎（EAE）的大脑和脊髓的炎症和免疫力，一种鼠模型 使用单克隆抗体的多发性硬化症。莱昂纳多·托内利（Leonardo Tonelli）博士的项目研究 部署和暴露于创伤事件，使士兵面临的风险比公众更大 发展心理障碍。 Tonelli博士的分析将包括相邻的免疫组织化学 部分：归因标记，趋化因子受体，小胶质细胞和巨噬细胞激活和/或 分化 - 所有这些都可以使用Keyence BZ-X800查看。 Guofeng Xie，医学博士研究结肠 癌症及其长期目标是通过针对关键信号来开发创新，有效和安全的疗法 结肠癌进展的基础转导分子。显微镜将允许Xie博士检查 使用免疫荧光抗MMP7抗体中基质金属蛋白酶7（MMP7）的表达模式 人类结肠癌细胞和手术标本。资助的项目构成了翻译研究的基础 这将改善退伍军人的治疗方法 - 潜在的新疗法。