ShEEP Request for Keyence BZ-X800E All-in-One Automated Imaging System

ShEEP 请求 Keyence BZ-X800E 一体化自动化成像系统

• 批准号: 9793454
• 负责人: TIBOR KRISTIAN
• 金额: --
• 依托单位: BALTIMORE VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9793454
• 关键词: 3-Dimensional; Agonist; Antibodies; Area; Baltimore; Bioenergetics; Brain; Brain Injuries; Brain Ischemia; Brain-Derived Neurotrophic Factor; Cell Count; Cell Death; Chronic; Clinical Sciences; Colon Carcinoma; Color; Comb animal structure; Computer software; Contracts; Development; Dimensions; Doctor of Medicine; Doctor of Philosophy; Encephalomyelitis; Equipment; Event; Exposure to; Failure; Fluorescence; Funding; General Population; Goals; Head; Health; Histologic; Homing; Human; Hybrid Cells; Image; Imagery; Immunity; Immunohistochemistry; Incubators; Inflammation; Injury; Intuition; Ischemia; Ischemic Brain Injury; Laboratory Research; Lead; Macrophage Activation; Maintenance; Matrilysin; Measurement; Medical center; Mental disorders; Methods; Microglia; Microscope; Mitochondria; Modeling; Monitor; Monoclonal Antibodies; Multiple Sclerosis; Oils; Operative Surgical Procedures; Organ; Organelles; Pattern; Pharmacotherapy; Phase; Price; Principal Investigator; Process; Program Evaluation; Rehabilitation Research; Reperfusion Injury; Reperfusion Therapy; Request for Proposals; Research; Research Personnel; Resolution; Risk; Scanning; Series; Services; Shapes; Shipping; Signal Transduction; Soldier; Specimen; Spinal Cord; Stress; Stroke; System; Testing; Texas red; Thick; Three-dimensional analysis; Time; Tissues; Training; Translational Research; Traumatic Brain Injury; Veterans; Work; cancer cell; chemokine receptor; colon cancer progression; contrast imaging; cost; cyanine dye 5; design; discount; fluorescence imaging; fluorescence microscope; high resolution imaging; imaging system; improved; innovation; instrument; interest; lens; live cell imaging; medical specialties; mouse model; neuroinflammation; novel; novel therapeutics; processing speed; research and development; stroke victims; targeted treatment; three dimensional structure; tissue processing; tool; translational study; traumatic event

The objective of this Shared Equipment Evaluation Program (ShEEP) proposal is to request Keyence BZX800E All-in-One Automated Imaging System which will offer researchers an instrument that is capable of: confocal-like multi-stack imaging and real-time focusing of thick tissue sections; rapid automatic high- magnification image acquisition and stitching of composite images; and three-dimensional profiling of serial tissue sections imaged at high resolution. The control software is simple and intuitive. Thus, with minimal training a novice user can scan a series of tissue sections from an entire organ, generate high-resolution composites of each tissue section, and merge all composites into a single three-dimensional rendering of the entire tissue. Traditional microscopes available at the Baltimore VA Medical Center (BVAMC) do not possess these capabilities. The VA projects cover an array of specialties as described below. Each will benefit from Keyence BZX800E All-in- One Automated Imaging System. The BZX800E Microscope optimizes the imaging process by designing a fully automated, easy-to-use system that captures high-resolution images and allows researchers to achieve their desired results faster. These applications are of great use for VA Biomedical Laboratory Research and Development Service (BLR&D), Clinical Science Research and Development (CSR&D), and Rehabilitation Research and Development (RR&D) funded investigators. Tibor Kristian, Ph.D. will serve as the Project Director (PD)/ Principal Investigator (PI) for this proposal. Dr. Kristian’s study focuses on understanding injury mechanisms that lead to mitochondrial and ultimately cellular bioenergetic failure in stroke victims. This imaging system is essential for imaging and quantification of intracellular organelles damage following stroke- induced ischemic brain injury. Bingren Hu, M.D., Ph.D. is testing a novel hypothesis that brain ischemia- reperfusion leads to a cascade of events resulting in inactivation; as well as study novel mechanisms underlying brain ischemia-reperfusion (IR) injury. Many antibodies will be used in Aims 1 and 2 of Dr. Hu’s studies; and the BZX800 microscope is necessary for imaging and quantification of intracellular organelles damage following IR brain injury. Bogdan Stoica, M.D. will use this new system to investigate traumatic brain injury (TBI). Dr. Stoica’s project requires significant imaging of chronic neuroinflammation markers and secondary damaging markers. Tapas Makar, Ph.D.’s project studies the effects of 7, 8- dihydroxyflavone (7,8- DHF); which has potent brain derived neurotrophic factor (BDNF) agonist activity and will use the imaging system in his specific aims 2 and 3. Dr. Makar will determine whether DHF treatment modulates inflammation and immunity in the brain and spinal cord in encephalomyelitis (EAE), a murine model of Multiple sclerosis, using monoclonal antibodies. Leonardo Tonelli, Ph.D.’s project studies the stress of deployment and exposure to traumatic events that puts soldiers at a greater risk than the general public in developing psychological disorders. Dr. Tonelli’s analysis will consist of immunohistochemistry in adjacent sections for: homing markers, chemokine receptors, microglia and macrophage activation and/or differentiation- all of which can be viewed with the Keyence BZ-X800. Guofeng Xie, M.D. studies colon cancer and his long-term goal is to develop innovative, effective, and safe therapies by targeting pivotal signal transduction molecules underlying colon cancer progression. The microscope will allow Dr. Xie to examine the expression patterns of matrix metalloproteinase 7 (MMP7) using immunofluorescent anti-MMP7 antibody in human colon cancer cells and surgical specimens. The funded projects form the basis for translational studies that will improve treatment in Veterans- potentially yielding new therapeutics.

此共享设备评估计划 (ShEEP) 提案的目标是要求 Keyence BZX800E 一体化自动成像系统将为研究人员提供一种能够： 厚组织切片的类共焦多堆栈成像和实时聚焦； 复合图像的放大图像采集和拼接；以及序列的三维分析； 组织切片以高分辨率成像，因此控制软件简单直观。 培训新手用户可以扫描整个器官的一系列组织切片，生成高分辨率 每个组织切片的复合材料，并将所有复合材料合并为单个组织切片的三维渲染 巴尔的摩退伍军人医疗中心 (BVAMC) 提供的传统显微镜不具备完整的组织。 这些能力。 VA 项目涵盖一系列专业，如下所述，每个项目都将从 Keyence BZX800E 中受益。 一体化自动成像系统 BZX800E 显微镜通过以下方式优化成像过程。 设计一个完全自动化、易于使用的系统，该系统可以捕获高分辨率图像并允许研究人员 这些应用对于 VA 生物医学实验室非常有用。 研究与开发服务 (BLR&D)、临床科学研究与开发 (CSR&D) 以及 Tibor Kristian 博士将担任康复研究与开发 (RR&D) 资助的研究人员。 该提案的项目总监 (PD)/首席研究员 (PI) 的研究重点是 Kristian 博士。 了解导致线粒体和最终细胞生物能衰竭的损伤机制 该成像系统对于细胞内细胞器的成像和定量至关重要。 中风引起的缺血性脑损伤后，胡秉仁博士正在测试一个新的假设。 脑缺血再灌注会导致一系列事件导致失活，这也是研究新颖的； 脑缺血再灌注 (IR) 损伤的机制 许多抗体将用于目标 1 和 2。 胡博士的研究；BZX800显微镜对于细胞内的成像和定量是必需的 Bogdan Stoica 医学博士将使用这一新系统来研究 IR 脑损伤后的细胞器损伤。 Stoica 博士的项目需要对慢性神经炎症进行显着的成像。 Tapas Makar 博士的项目研究了 7、8- 的影响。 二羟基黄酮 (7,8-DHF)；具有有效的脑源性神经营养因子 (BDNF) 激动剂活性 将在他的具体目标 2 和 3 中使用成像系统。Makar 博士将确定 DHF 治疗是否有效 调节脑脊髓炎 (EAE)（小鼠模型）中大脑和脊髓的炎症和免疫 Leonardo Tonelli 博士的项目研究了多发性硬化症的压力。 部署和遭受创伤性事件使士兵比普通公众面临更大的风险 托内利博士的分析将包括邻近疾病的免疫组织化学分析。 部分：归巢标记、趋化因子受体、小胶质细胞和巨噬细胞激活和/或 分化 - 所有这些都可以通过 Keyence BZ-X800 查看，谢国锋医学博士研究结肠。 他的长期目标是通过针对关键信号来开发创新、有效和安全的疗法 谢博士将利用显微镜来检查结肠癌进展的转导分子。 使用免疫荧光抗 MMP7 抗体观察基质金属蛋白酶 7 (MMP7) 的表达模式 人类结肠癌细胞和手术标本构成了转化研究的基础。 这将改善退伍军人的治疗——有可能产生新的疗法。