Assessing a Novel Paradigm of Inducing Cryotolerance

评估诱导低温耐受的新范式

基本信息

批准号：
8243062
负责人：
Ram Devireddy
金额：
$ 18.5万
依托单位：
LOUISIANA STATE UNIV A&M COL BATON ROUGE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8243062
关键词：
Adherence Adipocytes Adipose tissue Alkaline Phosphatase Allogenic Animals Antibodies Autologous Biochemical Biological Assay Cardiac Cell Survival Cell Therapy Cells Centrifugation Chemicals Chills Chondrocytes Clinical Colony-Forming Units Assay Colony-forming units Communities Cryopreservation Cryopreserved Cell Detection Development Digestion Dimethyl Sulfoxide Disease Dyspnea Effectiveness Fibroblasts Flow Cytometry Freezing Genes Goals HSPB1 gene Heart Heart Arrest Heat shock proteins Heat-Shock Response Hormonal Human Hypotension Hypoxia Immunoblotting Immunotherapy In Vitro Infusion procedures Kidney Failure Laboratories Leptin Link Molecular Chaperones Muscle Cells Nausea Neurologic Neurons Osteoblasts Osteocalcin Osteogenesis Pathway interactions Patients Physicians Plastics Process Protocols documentation RNA analysis Regimen Relative (related person)Reporting Reverse Transcriptase Polymerase Chain Reaction Safety Serum Specimen Stem cell transplant Stem cells Stimulus Surface Testing Tissues Toxic effect Translating Undifferentiated Western Blotting adiponectin adult stem cell base bone sialoprotein collagenase conditioning cost improved in vivo lipid biosynthesis mineralization novel oil red O osteogenic protein expression research study respiratory response standard of care

项目摘要

DESCRIPTION (provided by applicant):The practical goals of this study will be the development and optimization of a serum free cryoprotection medium with minimum levels of cryoprotectants. We intend to pursue these studies using a hypothesis-driven, mechanism-based experimental approach, i.e., the viability of the cryopreserved adult stem cells (ASCs), in the undifferentiated, adipocyte- or osteoblast- differentiated states, can be improved by increasing the pre-freeze expression of heat shock proteins (HSPs). PUBLIC HEALTH RELEVANCE: This proposal will determine the link between the expression of heat shock proteins and pre-freeze (control) and post-freeze (cryopreservation) potential (osteogenic and adipogenic) of human adipose tissue derived adult stem cells (ASCs).

描述（由申请人提供）：这项研究的实际目标是开发和优化具有最低冷冻保护剂水平的无血清冷冻保护介质。我们打算使用基于假设驱动的基于机制的实验方法进行这些研究，即，在未分化的，脂肪细胞或成骨细胞分化状态下，冷冻保存的成年干细胞（ASC）的生存能力可以通过增加热激蛋白（热激蛋白（HSPS）的预释放表达）来改善。公共卫生相关性：该提案将确定热休克蛋白与冰冻前（对照）和冻结后（冷冻保存）潜在（成骨和脂肪生成）的联系之间的联系之间的联系之间的联系之间的联系是人类脂肪组织衍生的成年干细胞（ASCS）的联系。