Gene Library Resource for the Sea Urchin S. purpuratus

海胆 S. purpuratus 基因库资源

基本信息

批准号：
8079202
负责人：
ERIC H DAVIDSON
金额：
$ 58.65万
依托单位：
CALIFORNIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-05-01 至 2016-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8079202
关键词：
Animals Asterina Binding Biological Biological Models Chromosome Mapping Collaborations Communities Comparative Study Consultations Custom DNA DNA Sequence Data Data Set Databases Development Developmental Cell Biology Developmental Gene Distant Embryo Engineering Gene Expression Gene Expression Profile Gene Library Gene Transfer Gene Transfer Techniques Genes Genome Genomics Gravidity Hand Head Health Services Research Human Genome In Vitro Knowledge Laboratories Laboratory Animals Libraries Lytechinus Maintenance Measurement Messenger RNA Methods Modeling Molecular Biology Mosaicism Peptides Preparation Proteomics RNA Reagent Recombinants Regulator Genes Research Research Infrastructure Resources Sea Urchins Sequence Analysis Services Source Starfish Strongylocentrotus purpuratus Structure Synthetic Genes System Technology base college data structure design experience falls genome sequencing improved instrumentation medical schools new technology next generation operation research study transcription factor web site

项目摘要

DESCRIPTION (provided by applicant): This revised proposal seeks support for the Gene Library Resource for the purple sea urchin, Strongylocentrotus purpuratus. This Resource provides the essential infrastructure, new technology, molecular biology support services, and both material and informational resources for the whole field of sea urchin research. The Resource is unique in the world, and it is fair to state that directly or indirectly it has impacted every sea urchin laboratory in the US and elsewhere as well. In the last five years the P40 provided essential technological and material support that made it possible for HGRI to obtain the genomic sequence of S. purpuratus. This animal has become a major research model, and knowledge of the genomically encoded regulatory networks underlying development of its embryo is more advanced than for any other animal system. Recently, HGRI approved support for sequencing of several further echinoderm species (at the Human Genome Sequencing Center, Baylor College School of Medicine). This decision was taken because of the major scientific advantages that would be available for the research community were the genomes of these additional echinoderm species for comparative studies of gene regulatory network organization, for cis-regulatory structure-function studies, and for research on mechanisms of embryonic and postembryonic development. But research model systems are useful in these fields only if the biological parameters of operation in the system as well as the sequence of the genome are in hand. The service and research objectives of the present renewal application thus fall into two broad components: to further support and develop the S. purpuratus research resource, by provision of advanced technologies and databases, and to establish equivalent technological and biological as well as material resources for the additional echinoderm species, while providing essential support for the genome sequencing efforts now being directed at these species. The initial set of additional species with which we are here concerned, all excellent laboratory animals, are a sea star, Asterina miniata, another very widely used sea urchin, Lytechinus varieaatus, and a distantly diverged echinoid, Eucidaris tribuloides. PUBLIC HEALTH RELEVANCE (provided by applicant): This enterprise is the principal source of genomic materials and information for the cell and developmental biology research community using the sea urchin biomedical model.

描述（由申请人提供）：该修订后的提案寻求支持紫色海胆，purpuratus的基因库资源。该资源为整个海胆研究领域提供了必不可少的基础设施，新技术，分子生物学支持服务以及物质和信息资源。该资源在世界上是独一无二的，公平地说，直接或间接地影响了美国和其他地方的每个海胆实验室。在过去的五年中，P40提供了必不可少的技术和材料支持，使HGRI获得了紫癜链球菌的基因组序列。该动物已成为主要的研究模型，并且对其胚胎开发的基因组编码的调节网络的了解比其他任何动物系统都要先进。最近，HGRI批准了对几种其他棘皮动物物种进行测序的支持（在贝勒学院医学院的人类基因组测序中心）。这一决定之所以做出，是因为研究界的主要科学优势是这些其他棘皮动物物种的基因组，用于基因调节网络组织的比较研究，用于顺式调节结构 - 功能研究，以及用于胚胎和胚胎后发展机制的研究。但是，仅当系统中的运行生物学参数以及基因组的序列都在手中，研究模型系统才在这些领域中有用。因此，当前续签应用的服务和研究目标属于两个广泛的组成部分：通过提供先进的技术和数据库，进一步支持和开发紫over虫研究资源，并为其他echinoderm物种建立等效的技术和生物学以及为其他echinoderm物种建立同等的物质资源，同时为这些物种提供了基于基础测序工作的基本支持。我们在这里与之相关的最初的其他物种，所有优秀的实验室动物，都是海星，Asterina Miniata，另一个非常广泛使用的海胆，Lytechinus varieaatus，以及远距离差异的棘突，Eucidaris tribuloides。公共卫生相关性（由申请人提供）：该企业是使用海胆生物医学模型的细胞和发育生物学研究界的基因组材料和信息的主要来源。