RESOURCE CORE 2 - BIOLOGICAL STUDIES CORE

资源核心 2 - 生物学研究核心

• 批准号: 8205992
• 负责人: Virginia Kraus
• 金额: $ 15.85万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8205992
• 关键词: Address; Age; Aging; American; Amino Acids; Animals; Arthritis; Biochemical; Biochemical Markers; Biochemical Process; Biological; Biological Aging; Biological Assay; Biological Markers; Carnitine; Cells; Cellular Assay; Chinese People; Clinical; Clinical Research; Clinical Trials; Cohort Studies; Communities; Core Facility; Counseling; Cross-Sectional Studies; Data; Databases; Development; Disease; Early identification; Elderly; Exercise; Functional disorder; Funding; Genetic Markers; Glycine; Goals; Grant; Health; Health Professional; Human; Individual; Instruction; Insulin Resistance; Intervention; Knowledge; Lead; Lipids; Longevity; Maintenance; Measures; Mediator of activation protein; Metabolic; Metabolic Marker; Metabolism; Methods; Mitochondria; Modification; Molecular; Molecular Profiling; Morbidity - disease rate; Muscle Mitochondria; Pathway interactions; Physical Function; Process; Publications; Research; Resources; Risk; Risk Factors; Sampling; Supplementation; Testing; Time; Tissues; Training; Translating; Urine; Work; adduct; age related; base; clinically relevant; cohort; collaboratory; combat; fitness; functional decline; functional outcomes; functional status; insight; investigator training; laboratory facility; metabolomics; mortality; novel marker; novel strategies; prognostic; programs; therapy design; trend

The purpose of the Biological Studies Core (RC2) facility is to provide a centralized resource for biomarker analyses in support of Duke Pepper OAIC projects to evaluate biomarker profiles as mediators and predictors of function and functional decline. This Core will provide a comprehensive laboratory facility for assays of cellular, molecular, biochemical and metabolomic factors related to aging and functional decline, inflammation and joint tissue metabolism.. The proposed Core Facility will serve in these capacities and will provide biomarker analyses to three large new externally funded studies in human cohorts to validate associations, discovered through the Duke Pepper OAIC in the previous 5 years, of physical function and functional decline with biomarkers, and to expand, enhance and refine knowledge related to these biomarkers as potential mediators and predictors of function. These three cohorts will afford the opportunity to: 1) assess the strength of the prognostic capability of the biomarkers for predicting risk of functional decline or mortality over the subsequent 1-3 years; 2) assess concurrent change in biomarkers and function in a community sample with acquisition of baseline and 24-month longitudinal samples; 3) assess both concurrent change in biomarkers and functional status and the impact of an exercise intervention on biochemical and metabolic markers as well as physical function. The Core Facility will also initially support one Developmental Project to advance the understanding of metabolic function and aging. This project will focus on developing a targeted mass spectroscopic analytic method for acylglycines in urine and will evaluate concurrent change in this new marker and metabolic health and insulin resistance with a clinical trial intervention of glycine supplementation in animals. This Core will also continue to serve as a resource for the training of investigators on principles and methods of biomarker analyses. These biomarker studies are expected to provide further insights into the molecular pathophysiology of aging to inform potential new strategies for intervention and modification of functional decline. The overall approach will continue to be one of a 'Collaboratory' in which multiple excellent clinical studies will contribute subsets of samples for biomarker analyses by the Duke Pepper OAIC Biological Studies Core, and statistical analysis of results by the Duke Pepper OAIC Analysis Core, to address, jointly and severally, the overarching Duke Pepper OAIC theme of exploring approaches to understand and modify multiple pathways of functional decline, and to translate noteworthy findings into disseminated knowledge through publications and advances in health.

生物学研究核心（RC2）设施的目的是为生物标志物分析提供集中资源，以支持Puke Pepper OAIC项目，以评估生物标志物概况作为介体和功能和功能下降的预测指标。该核心将提供一个全面的实验室设施，用于对与衰老和功能下降，炎症和联合组织代谢相关的细胞，分子，生化和代谢组分的测定。.拟议的核心设施将在这些能力中服务，并将为三个大型新型研究中的人类研究，以验证人类的研究，以验证人类的研究，以验证人类的研究，以验证人类的研究，该研究是在人类研究中，该研究的oa oa oa oa oa oa oa oa oa oa oa oa oa oa oa oa oa oa oa oa osia in oa oa oa osia ceppers oa oa op oa oa osia is的核心设施将在这些能力中提供。生物标志物的身体功能和功能下降，并扩展，增强和完善与这些生物标志物相关的知识，作为功能的潜在介体和预测指标。这三个队列将有机会：1）评估生物标志物预测能力的强度，以预测随后的1 - 3年中功能下降或死亡率的风险； 2）评估以下基线和24个月纵向样本的社区样本中生物标志物和功能的同时变化； 3）评估生物标志物和功能状态的并发变化以及运动干预对生化和代谢标记物以及身体机能的影响。核心设施最初也将支持 一个开发项目，以促进对代谢功能和衰老的理解。该项目将着重于为尿液中的酰基甘氨酸开发目标质谱分析方法，并通过对动物补充甘氨酸的临床试验干预来评估这种新标记和代谢健康和胰岛素抵抗的并发变化。该核心还将继续作为研究人员对生物标志物分析原理和方法进行培训的资源。预计这些生物标志物研究将为衰老的分子病理生理学提供进一步的见解，以告知潜在的新策略，以进行干预和功能下降的改变。总体方法将继续是一种“协作”之一，在该方法中，多项出色的临床研究将为生物标志物分析的样本子集贡献用于生物标志物分析的子集，以及对杜克·胡椒（Duke Pepper Oaic）分析核心结果对结果进行统计分析，以共同和偏僻的是，探索杜克·帕珀（Duke Pepper Oaic）的主题和修饰方法，以探讨和偏僻的降低，以探索和修饰的方法，以探索和修饰的方式，以探索和修饰的方式。通过出版物和健康的进步进入传播知识的结果。