Molecular Biology/Molecular Genetics Core

分子生物学/分子遗传学核心

基本信息

批准号：
8135912
负责人：
RUDOLPH L LEIBEL
金额：
$ 17.69万
依托单位：
ST. LUKE'S-ROOSEVELT INST FOR HLTH SCIS
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2016-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8135912
关键词：
Animal Model Architecture Biology Biomedical Research Body Composition Body Weight Capital Cells Cloning Comorbidity Complex Complications of Diabetes Mellitus Computer Analysis Computer software Congenic Mice Consult Costs and Benefits Data Dimensions Endocrine Equipment Faculty Feeding behaviors Funding Gene Expression Genes Genetic Genetic Variation Genomics Grant Homeostasis Human Human Resources In Vitro Individual Investigation Investments Laboratories Leptin Licensing Metabolic Mission Molecular Molecular Biology Molecular Genetics Mus Mutation New York Obesity Peer Review Phenotype Play Publications Reagent Research Research Personnel Resources Rodent Rodent Model Role Services Somatic Cell Stem cells Students Technical Expertise Techniques Technology Testing Tissues Training United States National Institutes of Health Variant Work base cost energy balance experience gene function genetic analysis human subject instrument leptin receptor member mouse genome mouse model nutrition programs tool

项目摘要

The mission of the Molecular Biology/Molecular Genetics (MBMG) Core is to assist investigators seeking to discover the molecular bases of obesity and its co-morbidities. Through collaborative efforts with other NYONRC Cores, the effects of defined genetic alterations in humans and rodents on ingestive behavior, energy balance, body composition, and endocrine function can be characterized. The role of candidate molecules in tissues and cells related to energy homeostasis can be delineated through experimentation on both human subjects and rodent models. Access to sophisticated manipulations of the mouse genome and to in vitro manipulation of genes are also critical to generating and testing hypotheses with regard to mechanisms by which genetic effects are conveyed. Finally, the use of stem cells to replicate the cell-based consequences of genetic variation is playing a rapidly growing role in biomedical research. The modern tools of molecular biology and molecular genetic analysis comprise rapidly expanding techniques for acquiring and interpreting large amounts of data relating to gene sequence, variation in genomic architecture, gene expression and gene function. These approaches require specialized, costly equipment, skilled technical personnel and increasingly intense and complex computational analysis, all of which are inefficiently replicated in multiple individual laboratories. The objectives of the MBMG Core are to minimize barriers to the integration of cutting edge technologies of molecular genetics and genomics into the research of the Center's constituencies; to allow experienced investigators as well as researchers who are not molecular geneticists to add this dimension to their studies at reasonable cost.

分子生物学/分子遗传学（MBMG）核心的任务是帮助寻求发现肥胖及其合并症的分子碱基的研究人员。通过与其他NyonRC核心的合作努力，可以表征人类和啮齿动物对摄入行为，能量平衡，身体成分和内分泌功能的定义遗传改变的影响。可以通过对人类受试者和啮齿动物模型进行实验来描述候选分子在组织和与能量稳态相关的细胞中的作用。获取对小鼠基因组的复杂操纵和基因的体外操纵，对于传达遗传效应的机制而产生和测试假设也至关重要。最后，使用干细胞复制基于细胞的后果遗传变异在生物医学研究中起着快速增长的作用。分子生物学和分子遗传分析的现代工具包括迅速扩展的技术，用于获取和解释与基因序列，基因组结构变异，基因表达和基因功能有关的大量数据。这些方法需要专业的，昂贵的设备，熟练的技术人员以及日益激烈且复杂的计算分析，所有这些都是在多个单个实验室中效率低下。 MBMG核心的目标是最大程度地降低分子遗传学和基因组学的最先进技术与中心选区研究的障碍；允许经验丰富的研究人员以及不是分子遗传学家的研究人员，以合理的成本将此维度添加到他们的研究中。