Tissue Culture Core Facility

组织培养核心设施

基本信息

批准号：
8340321
负责人：
Steve Oglesbee
金额：
$ 20.47万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-05-23 至 2015-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8340321
关键词：
Accounting Adipose tissue Aliquot Basic Science Bioinformatics Biology Biostatistics Core Blood Cancer Center Cancer Center Support Grant Cancer Patient Cancer Survivorship Cells Clinical Clinical Sciences Collection Computer software Consent Core Facility DNA Data Data Collection Databases Discipline Enrollment Epidemiology Faculty Freezing Funding Genomics Genotype Grant Guidelines Health Hematology Human Individual Institutional Review Boards Label Laboratories Link Malignant - descriptor Malignant Neoplasms Normal Statistical Distribution Nursing Faculty Operative Surgical Procedures Pathology Patients Peer Review Peer Review Grants PicoGreen Pilot Projects Plasma Population Sciences Process Proteomics Protocols documentation Quality Control RNA Recruitment Activity Registries Research Research Personnel Role Saliva Sampling Serum Services Solid Specimen Specimen Handling Spottings Surgical Pathology Swab The Sun Time Tissue Procurements Tissue Sample Tissues Translational Research Tube UNC Lineberger Comprehensive Cancer Center Urine Whole Blood Work anticancer research biobank cohort cost data management follow-up genome wide association study human subject member multidisciplinary next generation oncology patient registry research study software systems tissue culture

项目摘要

Tissue Procurement and Biospecimens Core Facility The major objective of the Tissue Procurement and Biospecimens Core Facility is to support and enhance cancer-related translational, clinical, population and basic science research. This is achieved by providing UNC Lineberger Comprehensive Cancer Center members with a centralized, coordinated, quality controlled, quality-assured facility for the procurement, processing, storage, and distribution of biologic specimens. Such specimens include normal and malignant tissue, blood, buccal samples, cell lysates, saliva, urine and adipose, collected from human subjects with and without cancer. The facility procures biologic specimens for utilizing its own IRB-approved protocols. The facility now serves over 50 IRB approved protocols. It will begin to serve the UNC Health Registry Cancer Survivorship Cohort protocol that incorporates a global consent process developed to support the enrollment into the planned 10,000 patient Cancer Survivorship Cohort with well-characterized, clinically annotated biologic specimens. The facility provides laboratory support and storage capabilities for both clinical and epidemiologic cancer research studies. For investigator driven studies, the core accommodates investigator specific needs and applications, including planning and performing experiments to optimize specimen handling. In addition, the facility supports many pilot studies and institutionally supported studies to obtain preliminary data for subsequent peer-reviewed grants, especially for newly recruited clinical faculty. During 2009, the facility collected tissue samples from 1094 patients and distributed 674 samples to LCCC members. A major service of the facility is DNA extraction, quantitation, normalization, quality analysis and aliquoting. Additionally, the core coordinates and integrates activities with several other cores, including the Translational Core, the Genomics Core, the Proteomics Core, the Next Generation Sequencing and Genotyping Core, the Biostatistics and Data Management Core, and the Bioinformatics Core. All aspects of sample processing are tracked, using a unique identifier, from arrival in the lab through processing, storage, and disbursement. Distribution of biologic specimens and con-esponding data is facilitated by the Lineberger Data-Biologies Governance/Sharing Committees and guidelines. We request $297,439 in CCSG funds, representing 20% of the core's operating costs for 2010. Over 90% of the tissues use was by LCCC members. Members accounted for 79 % of blood and other biospecimen use.

组织采购和生物测量核心设施组织采购和生物测量核心设施的主要目标是支持和增强与癌症相关的转化，临床，人群和基础科学研究。这是通过为UNC Lineberger综合癌症中心成员提供的集中式，协调，质量控制，质量鉴定的设施，用于生物标本的采购，加工，储存和分布。这样的标本包括正常和恶性组织，血液，颊样品，细胞裂解物，唾液，尿液和脂肪从有或没有癌症的人类受试者中收集。该设施采用生物标本来利用其自己的IRB批准协议。该设施现在为50多个IRB批准的协议提供服务。它将开始为UNC卫生注册表癌症生存队列协议提供服务，该协议纳入了全球同意程序，以支持计划入学的10,000名患者癌症生存队列具有良好的临床注释的生物标本。该设施为临床和流行病学癌症研究提供了实验室支持和存储能力。对于研究者驱动的研究，核心可容纳研究者的特定需求和应用，包括计划和执行实验以优化样品处理。此外，该设施支持许多试点研究和机构支持的研究，以获取初步数据随后经过同行评审的赠款，尤其是针对新招募的临床教师。在2009年期间，该设施收集了1094例患者的组织样品，并向LCCC成员分配了674个样品。该设施的主要服务是DNA提取，定量，归一化，质量分析和等分试样。此外，核心坐标并将活动与其他几个核心相结合，包括转化核心，基因组核心，蛋白质组学核心，下一代测序和基因分型核心，生物统计学和数据管理核心以及生物信息信息核心。使用唯一标识符从到达实验室，通过处理，存储和支出来跟踪样本处理的所有方面。 LineBerger数据生物治理/共享委员会和准则促进了生物标本和相应数据的分布。我们要求CCSG资金$ 297,439，占2010年核心运营成本的20％。超过90％的组织使用是LCCC成员。成员占血液和其他生物循环使用的79％。