Cellular and Molecular Neuroscience Core

细胞和分子神经科学核心

基本信息

批准号：
8257638
负责人：
John P Svaren
金额：
$ 31.92万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-26 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8257638
关键词：
Alexander Disease Astrocytes Biological Assay Biomanufacturing Biotechnology Cancer Center Cell Line Cells Clinical Commit Confocal Microscopy Core Facility DNA DNA Sequence DNA biosynthesis Developmental Disabilities Equipment Flow Cytometry Fluorescence Microscopy Funding Gel Generations Genomics Gifts Housing Human Human Cell Line In Situ Individual Informatics Intellectual functioning disability Laser Scanning Confocal Microscopy Mass Spectrum Analysis Mental Retardation and Developmental Disabilities Research Centers Microscopy Modeling Molecular Molecular and Cellular Biology Neurons Neurosciences Patients Preclinical Drug Evaluation Production Proteomics Research Research Personnel Resources Retinitis Pigmentosa Rett Syndrome Services Technology Training Viral Vector Vitelliform macular dystrophy chromatin immunoprecipitation cost effective design induced pluripotent stem cell interest new technology programs response small molecule stem cell biology two-dimensional

Alexander Disease Astrocytes Biological Assay Biomanufacturing Biotechnology Cancer Center Cell Line Cells Clinical Commit Confocal Microscopy Core Facility DNA DNA Sequence DNA biosynthesis Developmental Disabilities Equipment Flow Cytometry Fluorescence Microscopy Funding Gel Generations Genomics Gifts Housing Human Human Cell Line In Situ Individual Informatics Intellectual functioning disability Laser Scanning Confocal Microscopy Mass Spectrum Analysis Mental Retardation and Developmental Disabilities Research Centers Microscopy Modeling Molecular Molecular and Cellular Biology Neurons Neurosciences Patients Preclinical Drug Evaluation Production Proteomics Research Research Personnel Resources Retinitis Pigmentosa Rett Syndrome Services Technology Training Viral Vector Vitelliform macular dystrophy chromatin immunoprecipitation cost effective design induced pluripotent stem cell interest new technology programs response small molecule stem cell biology two-dimensional

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项目摘要

The Cellular and Molecular Neuroscience Core is designed to facilitate the research objectives of the IDDRC and the scientific progress of individual IDDRC projects by providing state-of-the-art equipment, training, and expertise for IDDRC investigators employing, or wishing to employ, cellular and molecular biology in their research programs. In-house equipment includes advanced microscopy (laser scanning confocal and fluorescence microscopy with Stereology), genomic and proteomic analysis both in situ (flow cytometry) and in cell lysates (phosphonmaging and quantitative PCR), and informatics. During the current project period, the Waisman Center committed $500,000 of gift funds to upgrade the Core's existing proteomics and confocal microscopy capabilities. In addition, the CMN Core has well-established partnerships with nearby UW-Madison core facilities (UW-Biotechnology Center and UW-Cancer Center) to offer cost effective, state-of-the-art genomic and proteomic technology, such as 2-dimensional gel analysis, mass spectrometry, DNA sequencing and synthesis, DNA array and SNP analysis, NextGen sequencing, tiled array analysis of chromatin immunoprecipitation (ChIP) assays, and small molecule drug screening. In response to the growing interest for human-derived IDD models, in 2009 we created a new service of the CMN Core (with ARRA support) to generate induced pluripotent stem cells (iPSCs) from individuals with IDD conditions for IDDRC investigators. The IPSC service leverages the IDDRC's long-standing expertise in stem cell biology, access to well characterized patients with IDD (via the Research Participation Core), and clinical biomanufacturing (Waisman Biomanufacturing) for the production of IPSC viral vectors. Using these complementary resources, the iPSC service has successfully created 23 cell lines from patients with IDD including Rett syndrome, Alexander disease, FXS, DS, SMA, retinitis pigmentosa. Best disease, and others, and provided guidance and expertise in differentiating the cell lines into neuronal sub-types and astrocytes.

细胞和分子神经科学核心旨在通过为IDDRC研究人员提供最先进的设备，培训和专业知识，以促进IDDRC的研究目标以及单个IDDRC项目的科学进步。内部设备包括高级显微镜（激光扫描共聚焦和荧光显微镜使用立体学），基因组和蛋白质组学分析原位（流式细胞仪）和细胞裂解物（磷酸化和定量PCR）以及信息学。在当前的项目期间，Waisman中心承诺了500,000美元的礼品资金来升级核心现有的蛋白质组学和共聚焦显微镜功能。此外，CMN Core与附近的UW-Madison建立了良好的合作伙伴关系核心设施（UW-Biotechnology Center和UW-Cancer中心）可提供具有成本效益的最先进的基因组和蛋白质组学技术，例如二维凝胶分析，质谱，DNA测序和合成，DNA阵列和SNP分析，NextGen测序分析，NextGen测序，薄层群群均型磨石药物分析（Chomatay Marecray ship）和CHIP（CHIP）。为了响应对人类衍生的IDD模型日益增长的兴趣，在2009年，我们创建了CMN核心的新服务（并提供ARRA支持），以生成IDDRC研究人员IDD条件的个体的诱导多能干细胞（IPSC）。 IPSC服务利用IDDRC在干细胞生物学方面的长期专业知识，访问具有IDD的良好患者（通过研究参与核心）以及临床用于生产IPSC病毒载体的生物制造（Waisman生物制造）。使用这些互补资源，IPSC服务已成功创建了IDD患者的23种细胞系，包括RETT综合征，Alexander病，FXS，DS，SMA，SMA，视网膜炎色素。最佳疾病和其他疾病，并提供了将细胞系区分为神经元亚型和星形胶质细胞的指导和专业知识。