Cross-kingdom RNA communications between plant and fungal pathogens

植物和真菌病原体之间的跨界RNA通讯

基本信息

批准号：
10797921
负责人：
Hailing Jin
金额：
$ 23.55万
依托单位：
UNIVERSITY OF CALIFORNIA RIVERSIDE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-15 至 2025-08-31
项目状态：
未结题

项目摘要

SUMMARY Fungal pathogens infect humans, animals, and plants and cause severe consequences on global human health and crop production. Communication between hosts and pathogens is essential for host defense and pathogen virulence, but the underlying mechanisms are not well understood. Previous studies in my lab discovered that some non-coding regulatory small RNAs (sRNAs) from fungal pathogens, such as Botrytis cinerea, which causes grey mold disease on more than 1000 plant species, can be transported into host plant cells and suppress host immunity genes, a mechanism called “Cross-Kingdom RNAi”. Recently, we discovered that such sRNA communication is bi-directional. Plant hosts have also developed the ability to deliver sRNAs, mainly using extracellular vesicles, to fungal cells and induce cross-kingdom RNAi of fungal virulence-related genes. Such sRNA communication was also observed between mammals and parasites. Although more and more studies across diverse systems demonstrate that mobile sRNAs are key regulatory molecules in host and pathogen interactions, the field of cross-kingdom/cross-species RNA communication is still in its infancy. This proposal is designed to use plant Arabidopsis and fungal pathogen Botrytis as a model system to address the outstanding questions in this field, including, how host cells control sRNA transport upon infection, how specific classes of small RNAs are sorted into extracellular vesicles, how fungal cells deliver sRNAs into host cells, what are the mechanisms of RNA and vesicle uptake in the host cells and fungal cells, and whether other classes of RNAs, such as mRNAs and long non-coding RNAs, move between host and fungal cells, how they function in the counter party, etc.. A combination of genetics, genomics, biochemical and molecular biology approaches will be used. This project is expected to provide unprecedented insight into the underlying mechanisms of cross-kingdom/cross-species RNA communications, which will ultimately help develop innovative and eco-friendly disease control strategies and RNA-based fungicides or antifungal drugs.

概括真菌病原体感染人类、动物和植物，并对人类造成严重后果全球人类健康和作物生产之间的交流。对于宿主防御和病原体毒力至关重要，但其潜在机制并不重要我实验室之前的研究发现了一些非编码监管。来自真菌病原体的小 RNA (sRNA)，例如灰葡萄孢 (Botrytis cinerea)，它会导致灰色超过 1000 种植物的霉菌病，可以被转运到宿主植物细胞中并宿主抑制免疫基因，这种机制被称为“跨界RNAi”。发现这种 sRNA 通讯也是双向的。开发了主要使用细胞外囊泡向真菌细胞递送 sRNA 的能力并诱导真菌毒力相关基因的跨界RNAi。还观察到哺乳动物和寄生虫之间的交流。跨不同系统的更多研究表明，移动 sRNA 是关键的调控宿主和病原体相互作用中的分子，跨界/跨物种RNA领域该提案旨在利用植物拟南芥。和真菌病原体 Botrytis 作为模型系统来解决本研究中的突出问题领域，包括宿主细胞如何在感染时控制 sRNA 运输、特定类别如何的小 RNA 被分选到细胞外囊泡中，真菌细胞如何将 sRNA 传递到细胞外囊泡中宿主细胞，宿主细胞和真菌中RNA和囊泡摄取的机制是什么细胞，以及其他类别的 RNA，例如 mRNA 和长非编码 RNA，在宿主细胞和真菌细胞之间移动，它们如何在对方细胞中发挥作用，等等。遗传学、基因组学、生物化学和分子生物学方法的结合该项目预计将提供对底层的前所未有的洞察。跨界/跨物种 RNA 通讯机制，最终将帮助开发创新且环保的疾病控制策略和基于 RNA 的策略杀菌剂或抗真菌药物。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

RNAs - a new frontier in crop protection.

RNA——作物保护的新领域。

DOI：
10.1016/j.copbio.2021.06.005
发表时间：
2021-06-30
期刊：
Current opinion in biotechnology
影响因子：
7.7
作者：
Dongdong Niu;R. Hamby;J. Sánchez;Qiang Cai;Q. Yan;Hailing Jin
通讯作者：
Hailing Jin

Beyond transcription: compelling open questions in plant RNA biology.

超越转录：植物 RNA 生物学中引人注目的开放性问题。

DOI：
发表时间：
2023-05-29
期刊：
影响因子：
0
作者：
Manavella, Pablo A;Godoy Herz, Micaela A;Kornblihtt, Alberto R;Sorenson, Reed;Sieburth, Leslie E;Nakaminami, Kentaro;Seki, Motoaki;Ding, Yiliang;Sun, Qianwen;Kang, Hunseung;Ariel, Federico D;Crespi, Martin;Giudicatti, Axel J;Cai, Qiang;Jin
通讯作者：
Jin

Effective methods for isolation and purification of extracellular vesicles from plants.

从植物中分离和纯化细胞外囊泡的有效方法。

DOI：
10.1111/jipb.13181
发表时间：
2021-10-20
期刊：
Journal of integrative plant biology
影响因子：
0
作者：
Yifan Huang;Shumei Wang;Qiang Cai;Hailing Jin
通讯作者：
Hailing Jin

Message in a Bubble: Shuttling Small RNAs and Proteins Between Cells and Interacting Organisms Using Extracellular Vesicles.

气泡中的信息：使用细胞外囊泡在细胞和相互作用的生物体之间穿梭小 RNA 和蛋白质。

DOI：
发表时间：
2021
期刊：
影响因子：
0
作者：
Cai, Qiang;He, Baoye;Wang, Shumei;Fletcher, Stephen;Niu, Dongdong;Mitter, Neena;Birch, Paul R J;Jin, Hailing
通讯作者：
Jin, Hailing

Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis.

真菌小 RNA 乘坐在细胞外囊泡中，通过网格蛋白介导的内吞作用进入植物细胞。