Kidney single cell and spatial molecular atlas project - KIDSSMAP

肾脏单细胞和空间分子图谱项目 - KIDSSMAP

• 批准号: 10705733
• 负责人: Sanjay Jain
• 金额: $ 46.42万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10705733
• 关键词: 3-Dimensional; Age; Anatomy; Atlases; Biological Assay; Cells; Characteristics; Chromatin; Communities; Complex; Data; Data Analyses; Data Set; Dimensions; Disease; Dissociation; Ensure; Generations; Genetic Transcription; Goals; Human; Human BioMolecular Atlas Program; Individual; Infrastructure; Kidney; Kidney Diseases; Life Cycle Stages; Light; Lipids; Location; Machine Learning; Measurement; Metadata; Microanatomy; Microscopy; Modality; Modeling; Modernization; Molecular; Molecular Target; Names; Outcome; Production; Proteins; Quality Control; RNA; Race; Reporting; Resolution; Sampling; Site; Slice; Statistical Models; Structure; Subcellular Anatomy; Suspensions; System; Techniques; Tissue atlas; Tissues; Visualization; analysis pipeline; cell type; computer infrastructure; computerized tools; data integration; data sharing; data submission; experience; machine learning method; member; sex; single nucleus RNA-sequencing; supervised learning; tool; transcriptome

$EVWUDFW &RUH The KIDney Single cell and Spatial Molecular Atlas Project (KIDSSMAP) aims to generate an extensive spatially resolved 3D atlas of human kidney. This involves generation of high-resolution data capturing different classes of molecular moieties: from RNA and chromatin accessibility, to proteins, lipids and metabolites. These molecular facets will be assessed by a suite of different experimental techniques, applied to 3D tissue blocks, 2D sections, or dissociated cell suspensions. The central goal of the KIDney Data Analysis Core (KIDDAC) will be to integrate these data across different scales, molecular modalities, and a diverse set of individual characteristics (sex, age, race). This will be done on both the level of cellular and microanatomical structure annotation. KIDDAC will also coordinate uniform pre-processing and quality control (QC) of the individual modalities. Finally, production of a comprehensive tissue atlas will also require effective computational infrastructure for tracking of individual samples, execution of primary analysis pipelines, as well as automated submission and sharing of data with the HIVE/HuBMAP consortium and wider scientific community. The resulting atlas will illuminate principles underlying the complex microanatomical organization of the kidney and will provide a much-needed integrated baseline for characterizing aberrant states of the cells or tissue that might arise during disease.

$EVWUDFW&RUH 肾脏单细胞和空间分子图谱项目 (KIDSSMAP) 旨在生成广泛的 人类肾脏的空间分辨 3D 图谱。这涉及生成捕获不同数据的高分辨率数据 分子部分的类别：从 RNA 和染色质可及性到蛋白质、脂质和代谢物。这些 分子方面将通过一系列不同的实验技术进行评估，应用于 3D 组织块， 二维切片或解离的细胞悬浮液。 KIDney 数据分析核心 (KIDDAC) 的中心目标是 将这些数据整合到不同的尺度、分子模式和不同的个体中 特征（性别、年龄、种族）。这将在细胞和显微解剖结构水平上完成 注解。 KIDDAC 还将协调个人的统一预处理和质量控制 (QC) 方式。最后，综合组织图谱的制作也需要有效的计算 用于跟踪单个样本、执行主要分析流程以及自动化的基础设施 向 HIVE/HuBMAP 联盟和更广泛的科学界提交和共享数据。这 由此产生的图谱将阐明肾脏复杂的显微解剖组织的原理 将为表征细胞或组织的异常状态提供急需的综合基线 疾病期间可能会出现。