COMPUTATIONAL IMAGING CORE

计算成像核心

• 批准号: 7958995
• 负责人: DALLAS Melvin HYDE
• 金额: $ 3.56万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958995
• 关键词: Animals; Archives; Calendar; California; Client; Color; Computer Retrieval of Information on Scientific Projects Database; Computer Systems; Computer Workstations; Computer software; Computers; Confocal Microscopy; Consultations; Core Facility; Coupled; Data; Data Collection; Data Set; Data Storage and Retrieval; Databases; Development; Equilibrium; Equipment; Faculty; Film; Fluorescence; Frozen Sections; Funding; Future; Goals; Grant; Head; Hour; Housing; Image; Image Analysis; Institution; Lasers; Link; Location; Microscope; Microscopy; Microtome - medical device; Monitor; Online Systems; Paper; Paraffin; Pathology; Plastics; Preparation; Primates; Printing; Process; Production; Publications; Records; Research; Research Personnel; Reservations; Resolution; Resources; Sampling; Services; Source; Students; System; Training; Training Support; United States National Institutes of Health; Update; Vision; base; cryostat; digital imaging; fluorescence imaging; improved; innovation; instrument; instrumentation; light microscopy; method development; technique development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core facility provides light microscopy, stereology, digital imaging, image database development, and consultation services, primarily for the CNPRC, and also for all campus departments on a recharge basis. The goal is to assist faculty, staff, and students with their research needs for qualitative and quantitative applications. This Core assists with the production of publication quality images and offers consultation on experimental approaches for use with its equipment. Additionally, it is engaged in digitizing and correcting archived film images from various sources but primarily those created by the Pathology Service at the CNPRC. The Core also provides a service to annotate and upload fully corrected digital images into the CNPRC image database. Uploaded images are linked in the database to other information for a specific animal and made available to all who have been granted access. Instrumentation housed in the Core is available 24 hours a day, 7 days a week for trained CNPRC users. Consultation, training, and support service, along with access to the equipment for other departments, is available from 8-5 Monday through Friday. The facility houses the following equipment: * A Cryostat for frozen sections. * Microtomes for paraffin and plastic sectioning. * Sample preparation equipment for plastic processing and embedding. * Data storage servers (expandable multi-terabyte volumes) for archiving client, Pathology Unit and Core data. These systems provide rapid access to very large (multi-gigabyte) files or data sets that would otherwise require hours to transfer from other locations in the CNPRC. * Web-based calendar for instrument reservations. * An Olympus BX61 research microscope with Olympus MicroSuite image analysis software and Intelligent Imaging Innovations SlideBook software for stereology and image data collection. * A second Olympus BX61 microscope with Intelligent Imaging Innovations SlideBook software for stereology technique development by the Core's staff. * A graphics workstation, a flatbed scanner, and a high-quality color corrected printer for publication quality prints, converting film to digital images, image correction, and archiving. * Document scanners for rapid digitization and OCR conversion of paper records. * A Delta Vision Microscopy System and computer workstation for very high resolution multi-dimensional fluorescence imaging with deconvolution. * A computer workstation for offline analysis of stereology data (used for method development by the Core staff), and large multidimensional image analysis. * A recently updated Visiopharm VIS/CAST Grid Stereology System for quantitative data collection and automated image analysis. * Photoshop, Illustrator, LightRoom, and DreamWeaver software to support all digital imaging efforts of the Core  all computers are outfitted with spectrophotometrically color balanced monitors. * A fluorescence stereomicroscope outfitted with a high sensitivity camera coupled to a computer system. * A dual-head brightfield stereomicroscope. Future additions: The Core is in the process of purchasing a non-laser based confocal attachment for one of the Olympus BX61 microscope systems to improve the quality of fluorescent images. The Core hopes to purchase a widefield zoom confocal microscopy system for applications that require a very wide range of magnifications.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该核心设施主要针对CNPRC以及所有校园部门提供光学显微镜，立体学，数字成像，图像数据库开发和咨询服务。目的是协助教师，教职员工和学生对定性和定量应用的研究需求。该核心有助于生产出版质量图像，并就其设备使用的实验方法进行咨询。 此外，它还参与数字化和纠正来自各种来源的存档胶片图像，但主要是由CNPRC的病理服务创建的胶片图像。 核心还提供了一项服务，以注释和上传完全校正的数字图像到CNPRC图像数据库中。 上传的图像在数据库中链接到其他特定动物的其他信息，并提供给所有被授予访问权限的人。 核心中容纳的仪器每周24小时，每周7天，训练有素的CNPRC用户。咨询，培训和支持服务以及对其他部门的设备的访问，可从周一至周五8-5开始。 该设施容纳以下设备： *冷冻部分的低温恒温器。 *用于石蜡和塑料切片的微滴定。 *用于塑料加工和嵌入的样品准备设备。 *归档客户端，病理单元和核心数据的数据存储服务器（可扩展的多保持量量）。 这些系统可快速访问非常大的（多gabyte）文件或数据集，否则这些文件集将需要数小时从CNPRC中的其他位置转移。 *仪器预订的基于Web的日历。 * Olympus BX61研究显微镜，使用Olympus Microsuite图像分析软件和智能成像创新幻灯簿软件，用于立体和图像数据收集。 *第二个Olympus BX61显微镜，具有智能成像创新的幻灯箱软件，用于核心员工的立体学技术开发。 *图形工作站，平板扫描仪和高质量的颜色校正打印机，用于出版质量印刷品，将膜转换为数字图像，图像校正和归档。 *记录扫描仪，以快速数字化和OCR转换纸记录。 *用于高分辨率多维荧光成像的三角洲视觉显微镜系统和计算机工作站，具有反向卷积。 *用于离线分析的计算机工作站（由核心人员使用用于方法开发）和大型多维图像分析。 *最近更新的Visiopharm Vis/cast网格立场系统，用于定量数据收集和自动图像分析。 * Photoshop，Illustrator，Lightroom和Dreamweaver软件，以支持核心的所有数字成像工作，所有计算机都配备了分光光度计量的颜色平衡显示器。 *荧光立体显微镜，配备了高灵敏度相机，耦合到计算机系统。 *双头Brightfield立体显微镜。 未来增加： 核心是在为Olympus BX61显微镜系统之一购买非激光的共聚焦附件以提高荧光图像的质量。 核心希望为需要非常广泛的宏伟速度的应用购买广场变焦共聚焦显微镜系统。