Animal Core

动物核心

• 批准号: 7917193
• 负责人: ROBERT C MALENKA
• 金额: $ 10.96万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7917193
• 关键词: Adult; Age; Animal Behavior; Animals; Behavioral; Behavioral Assay; Biochemical; Biological; Biological Assay; Brain; Breeding; Cells; Characteristics; Consultations; Core Facility; Cost Savings; Embryo; Ensure; Event; Generations; Genes; Genotype; Goals; Grant; Harvest; Individual; Internet; Knock-out; Laboratories; Lead; Methods; Molecular; Monitor; Mus; Mutant Strains Mice; Mutation; Pattern; Principal Investigator; Procedures; Property; Proteins; Quality Control; Reagent; Research Personnel; Resources; Role; Scientist; Series; Shipping; Ships; Sum; Supervision; Synapses; Synaptic plasticity; Time; Transgenic Organisms; Universities; Update; cost; design; experience; insight; interdisciplinary approach; member; multidisciplinary; mutant; presynaptic; programs; research study

Core B: Animal Core Genetically modified mice offer many advantages for the multidisciplinary analysis of the mechanisms and functions of synaptic plasticity. They provide a renewable, reproducible and reliable resource that can be analyzed at multiple levels by multiple investigators with complementary expertise. Thus, a critical component of this program project is the use of the same lines of RIM mutant mice by all investigators. This will allow exploration of RIM function at multiple levels simultaneously, an approachwhich should synergistically lead to a more sophisticated understanding of the role of RIM proteins in active zone function and various forms of presynaptic plasticity. Importantly, by examining these mice behaviorally in well- defined assays of experience-dependent plasticity, we will also be able to probe the specific functional role of presynaptic LTP and the other forms of synaptic plasticity in which RIMs are involved. To accomplish this ambitious multidisciplinary approach will require a stable supply of correctly genotyped RIM mutant mice that can be used simulaneously by all members of the program project. The critical purpose of this animal core is to provide such animals. This Core will have two components, one at the Sudhof laboratory at UT Southwestern and one at Stanford. The UT Southwestern core will be responsible for the generation and initial characterization of the various mutant mice at the anatomical and biochemical levels. Mice will then be shipped to the Stanford Mouse Core B in which colonies will be established so that they can be examined in the cell biological, electrophysiological and behavioral assays described in Projects 2-4. Centralizing this function will achieve maximal efficiency both in terms of costs and labor and thus will greatly facilitate the overall progress of this program project.

核心B：动物核心 转基修饰的小鼠为机制的多学科分析提供了许多优势 突触可塑性的功能。他们提供可再生，可重现和可靠的资源 由具有互补专业知识的多个研究人员在多个级别进行了分析。因此，一个批判 该程序项目的组成部分是所有研究人员使用相同的RIM突变小鼠。这 将允许同时在多个级别上探索RIM功能，该方法应 协同地导致对RIM蛋白在活性区功能的作用的更复杂的理解 以及各种形式的突触前可塑性。重要的是，通过在良好的行为上检查这些小鼠 定义的依赖经验可塑性的测定法，我们还将能够探测 突触前LTP和涉及轮辋的其他形式的突触可塑性。实现这一目标 雄心勃勃的多学科方法将需要稳定的正确基因分型的边缘突变体小鼠， 程序项目的所有成员都可以模拟使用。该动物核心的关键目的是 提供这样的动物。该核心将有两个组件，一个在UT的Sudhof实验室 西南，一个在斯坦福大学。 UT西南核心将负责一代和 各种突变小鼠在解剖学和生化水平上的初始表征。然后老鼠将是 运送到斯坦福老鼠核心B，将建立殖民地，以便可以在 项目2-4中描述的细胞生物学，电生理和行为测定。集中这个 功能将在成本和劳动方面达到最大效率，因此将极大地促进 该计划项目的总体进展。