National Resource for Aplysia

海兔国家资源

• 批准号: 8051619
• 负责人: MICHAEL C SCHMALE
• 金额: $ 51.96万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8051619
• 关键词: Adult; Afferent Neurons; Age; Aging; Agonist; Alzheimer' s Disease; Animal Experimentation; Animals; Aplysia; Aplysia species; Area; Basic Science; Behavior; Biological Models; Biomedical Research; California; Cells; Communities; Containment; D Aspartate; Development; Diet; Disease; Documentation; Environment; Food; Goals; Harvest; Health; Health Status; Laboratories; Larva; Learning; Life; Marines; Measurement; Measures; Modeling; Monitor; Nervous system structure; Neurobiology; Photoperiod; Prevention; Process; Production; Reflex action; Reliance; Research; Research Personnel; Resources; Role; Sales; Screening procedure; Shipping; Ships; Snails; Specimen; Staging; Study models; Syndrome; Tail; Temperature; Time; Tissues; Universities; Withdrawal; animal resource; base; cost; egg; improved; meetings; neurophysiology; operation; pathogen; programs; public health relevance; rapid detection; research study; voltage clamp; water quality

DESCRIPTION (provided by applicant): This proposal describes the continuation of the National Resource for Aplysia, whose overall goal is to provide consistently high-quality cultured sea hares Aplysia californica (and their cultured red algal food) to researchers. A. californica is an important non-vertebrate (Opisthobranch mollusk) model system for health- related research, primarily in the neurophysiology of behavior and learning. We will continue to produce animals for research and to conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-projects/specific aims: 1. Production - Sales of animals to researchers in the US and internationally is projected to continue at present levels of 25,000 to 30,000 animals per year. This resource makes available high-quality A. californica at all life stages from eggs through mature adults by rearing animals in carefully controlled laboratory conditions. All life-stages are available in sufficient quantities to meet user demand throughout the year, eliminating reliance on wild-collected animals which have limited seasonal availability and are of unknown age and health status. 2. Production research programs - The resource has been continuously improved over the past 12 years by research to optimize rearing conditions for most life stages of A. californica. The proposed research program will focus on measurement and improvement of egg quality. 3. Animal Health Monitoring - We will continue a monitoring program based on screening animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animals at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied. 4. Developmental Neurophysiology - We will begin to develop Aplysia as an aging model by studying the changes in D-Aspartate- and other agonist-activated ionic currents that modulate sensory neuron input in the tail withdrawal reflex, using single cell voltage clamping and recording of the intact reflex. Through this combination of production and basic research, we will improve the model system and extend its usefulness to other areas of research. PUBLIC HEALTH RELEVANCE STATEMENT (provided by applicant): The California sea hare, Aplysia californica, is a large marine snail with a very simple nervous system. The National Resource for Aplysia raises and ships 25,000 to 30,000 of these animals per year to labs in the US and around the world for conducting basic research in neurobiology and behavior. This proposal would continue the production of these animals and expand their usefulness concerning studies of aging and Alzheimer's disease. D-Aspartate

描述（由申请人提供）：该提案描述了Aplysia国家资源的延续，其总体目标是向研究人员提供一致的高质量培养的Sea Hares Aplysia Aplysia Aplysia Aplysia（及其培养的红色藻类食品）。 A. californica是针对健康相关研究的重要非掌管脑（Opisthobranch Mollusk）模型系统，主要是在行为和学习的神经生理学中。我们将继续生产动物进行研究，并进行旨在探索新模型用途并改善资源的基础研究。有四个子项目/特定目的： 1。生产 - 预计每年25,000至30,000只动物的动物向美国和国际研究人员销售。该资源使从卵到成年成年人的所有生命阶段都可以在精心控制的实验室条件下饲养动物，从而提供高质量的A. californica。所有生命阶段都有足够的数量可满足全年用户需求，从而消除了对季节性供应有限且年龄和健康状况未知的野生动物的依赖。 2。生产研究计划 - 在过去的12年中，通过研究以优化A. Califortica的大多数寿命阶段的饲养条件，不断提高资源。拟议的研究计划将着重于卵质量的测量和改善。 3。动物健康监测 - 我们将继续基于筛查动物健康参数的监测计划，以确保在孵化场的任何动物发育阶段可能发生的任何疾病过程的快速检测和完整记录。任何观察到的疾病综合征和可疑病原体都将进行研究，并采用适当的控制措施。 4。发育神经生理学 - 我们将通过研究D-天冬氨酸和其他激动剂激活的离子电流的变化来开发作为衰老模型的Aplysia，这些变化使用单细胞电压夹紧和记录完整反射的单细胞电压夹紧和记录。 通过生产和基础研究的结合，我们将改善模型系统并将其有用性扩展到其他研究领域。 公共卫生相关性声明（由申请人提供）：加利福尼亚州野兔加利福尼亚州是一个大型海洋蜗牛，具有非常简单的神经系统。全国Aplysia资源每年将这些动物中的25,000至30,000只动物筹集到美国和世界各地的实验室，以进行神经生物学和行为的基础研究。该提议将继续生产这些动物，并扩大其对衰老和阿尔茨海默氏病的研究的有用性。 D-天冬氨酸