DESIGN/CONSTRUCT/TEST A NEW MASS SPECTROM FOR PROFILING PHOSPHORYLATION SITES

设计/构建/测试用于分析磷酸化位点的新质谱

• 批准号: 8361498
• 负责人: SUNNIE MYUNG
• 金额: $ 6.52万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361498
• 关键词: Coupled; Funding; Grant; International; Ions; Journals; Legal patent; Link; Mass Spectrum Analysis; National Center for Research Resources; Paper; Phosphorylation Site; Principal Investigator; Proteins; Publishing; Research; Research Infrastructure; Resources; Scanning; Source; Testing; Time; United States National Institutes of Health; Work; cost; design; design and construction; instrument; instrumentation; macromolecule; mass spectrometer; novel; prototype; simulation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have designed and constructed a novel mass spectrometer for profiling phosphorylation sites on proteins. The instrument is designed to be up to three orders of magnitude more sensitive than current commercially available instrumentation. We have tested the prototype and found that it works as per its design and our simulations. We have applied for and being granted a US patent for our novel design. A paper describing this work has been published: S. Myung, H. Cohen, D. Feny¿, J.C. Padovan, A.N. Krutchinsky, B.T. Chait. "High-capacity ion trap coupled to a time-of-flight mass spectrometer for comprehensive linked scans with no scanning losses", International Journal of Mass Spectrometry 2010. In press

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们设计并构建了一个新型的质谱仪，用于在蛋白质上分析磷酸化位点。该仪器的设计最多要比当前可商购的仪器更敏感。我们已经测试了原型，发现它根据其设计和我们的模拟工作。我们已申请并获得了美国专利的新颖设计专利。 一篇描述这项工作的论文已发表： S. Myung，H。Cohen，D。Feny¿，J.C。Padovan，A.N。 Krutchinsky，B.T。 Chait。 “高容量的离子陷阱耦合到飞行时间质谱仪，用于全面连接的扫描，没有扫描损失”，国际质谱杂志2010。