Assessing the accuracy of exposure measurements for common endocrine disrupting chemicals

评估常见内分泌干扰化学品暴露测量的准确性

• 批准号: 10697328
• 负责人: Patricia Hunt
• 金额: $ 15.39万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-06 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10697328
• 关键词: Address; Affect; Biological Monitoring; Blinded; Chemical Exposure; Chemicals; Collection; Coupled; Data; Dibutyl Phthalate; Dose; Endocrine Disruptors; Environmental Pollutants; Exposure to; Foundations; Funding; Glucuronides; Health; Human; Individual; Laboratories; Life; Measurement; Measures; Methodology; Methods; National Health and Nutrition Examination Survey; Ovary; Parabens; Pilot Projects; Pregnant Women; Research Design; Risk Assessment; Sampling; Second Pregnancy Trimester; Secure; Techniques; Urine; analytical method; analytical tool; bisphenol A; catalyst; cohort; epidemiologic data; epidemiology study; experimental study; exposed human population; fetal; insight; man; member; monobutyl phthalate; personal care products; safety assessment; sound

PROJECT SUMMARY Our ten-year project supporting the collection and analysis of human fetal ovaries was abruptly halted four years ago when high levels of BPA in some maternal urine samples in our cohort raised suspicion about our analytical prowess. We secured one year of R56 funding and used it to verify the accuracy of our new direct analytical method and demonstrate that older indirect methods on which human estimates of BPA exposure have been based do not accurately capture levels of the major BPA metabolite, BPA-glucuronide (BPA-G). Our data suggest human BPA exposure has been significantly underestimated. Further, because glucuronidated metabolites of other endocrine disrupting chemicals are measured using similar indirect methods, our findings raise concern that this may be a pervasive problem. The proposed pilot studies begin to address this concern by focusing on three chemicals that represent three different classes of endocrine disrupting chemicals. We will develop direct methods using authentic standards for the major metabolites of the three chemicals and use these methods to assess the accuracy of currently used indirect methods for measuring them in human biospecimens. We postulate that, like BPA, we will find inaccuracies in indirect analyses for at least two of the three. Because human biomonitoring data are used to obtain estimates of human exposure and these estimates, in turn, are essential in safety assessments, the proposed studies have the potential to affect not only human epidemiological studies designed to assess the effects of these chemicals on human health, but also to upend chemical risk assessment.

项目概要 我们为期十年的支持人类胎儿卵巢采集和分析的项目突然失败了 四年前停止，当时我们队列中一些母亲尿液样本中的 BPA 含量很高 引起了人们对我们分析能力的怀疑。我们获得了一年的 R56 资金并使用了它 验证我们新的直接分析方法的准确性并证明旧的间接分析方法 人类估计 BPA 暴露量所依据的方法不能准确捕捉 主要 BPA 代谢物 BPA-葡萄糖醛酸 (BPA-G) 的水平。我们的数据表明人类 BPA 暴露被严重低估。此外，由于葡萄糖醛酸化代谢物 我们的发现使用类似的间接方法测量其他内分泌干扰化学物质 引起人们的关注，这可能是一个普遍的问题。拟议的试点研究开始解决 通过关注代表三种不同类别内分泌的三种化学物质来解决这一问题 破坏化学物质。我们将使用专业的真实标准开发直接方法 三种化学物质的代谢物，并使用这些方法来评估当前的准确性 使用间接方法在人类生物样本中测量它们。我们假设，像 BPA 一样， 我们会发现这三者中至少有两个的间接分析是不准确的。因为人类 生物监测数据用于获得人类暴露的估计值，而这些估计值反过来， 对于安全评估至关重要，拟议的研究不仅有可能影响 人类流行病学研究旨在评估这些化学物质对人类的影响 健康，而且还颠覆化学品风险评估。