FITNESS AND LONGEVITY IN EXERCISE: FLEX

运动中的健身和长寿：FLEX

基本信息

批准号：
7718097
负责人：
KRISANN K OURSLER
金额：
$ 0.41万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-03-01 至 2009-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7718097
关键词：
Acquired Immunodeficiency Syndrome Activities of Daily Living Adult Aerobic Aerobic Exercise Affect Aging Baltimore Body Composition Cardiovascular system Censuses Clinic Communities Computer Retrieval of Information on Scientific Projects Database Control Groups Cytochromes DNA Elderly Electron Microscopy Eligibility Determination Enrollment Exercise Female Functional disorder Funding Gender Goals Grant HIV Heart Rate Highly Active Antiretroviral Therapy Histology Institution Intervention Jordan Longevity Maryland Measures Medical center Metabolic Methods Mitochondria Morphology Muscle Myopathy Nuclear Outcome Oxidases Performance Phase Physical Function Physiological Polymerase Chain Reaction Population Prevention Procedures Randomized Randomized Controlled Clinical Trials Recording of previous events Recovery Recruitment Activity Research Research Personnel Resources Screening procedure Skeletal Muscle Source Stretching Succinate Dehydrogenase Testing Training United States National Institutes of Health Universities Week X-Ray Computed Tomography base design eligible participant fitness follow-up improved male older patient programs sarcopenia sedentary trial comparing volunteer

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this proposal is to investigate the mechanisms by which aging affects physical functioning and aerobic capacity in HIV-infected adults, and to determine if aerobic exercise training improves functional and cardiovascular performance. Results will be used to design and test specific exercise interventions for older adults infected with HIV. Our long-term objective is to devise effective prevention and treatment strategies that will improve and preserve function in this rapidly growing older patient population. Specific Aim: To determine the effect of 20 week aerobic exercise training (16 weeks endurance training plus 4 weeks short-sprint interval training) on skeletal muscle oxidative function and aerobic capacity in older HIV adults. We will conduct a randomized trial of 60 HIV subjects = 55 years. The following parameters will be assessed at baseline,16 weeks, and 20 weeks in the treatment group (N=30) and control group (N=30): a) aerobic capacity (VO2peak); b) functional performance; c) skeletal muscle oxidative function (Cytochrome C oxidase (COX) and succinate dehydrogenase (SDH); d) skeletal muscle mitochondrial morphology (electron microscopy) and mtDNA (mt/nuclear DNA ratio by PCR); e) lean body mass (DEXA;CT scan); f) skeletal muscle histology (HIV-related myopathy). Hypothesis 1. Skeletal muscle oxidative dysfunction is a mechanistic factor underlying reduced aerobic capacity and functional limitations in older HIV adults. Hypothesis 2. Aerobic exercise training will improve muscle oxidative dysfunction, and the associated functional limitations and reduced aerobic capacity in older HIV subjects compared to sedentary older HIV controls; however, improvements may be limited by the extent of sarcopenia and HIV-related myopathy. Design/procedure: This randomized trial compares the effect of 20-weeks aerobic exercise training versus stretching control in HIV-infected adults = 55 years. Outcomes include physiologic, functional, muscular, metabolic, and body composition measures which will be assessed at baseline, 16 weeks, and 20 weeks. All subjects will be HIV-infected and on stable highly active antiretroviral therapy (HAART). The majority of the volunteers will be recruited from the ID Clinic at the Baltimore VA Medical Center (VAMC)(census 620) and the University of Maryland adult HIV clinic, Evelyn Jordan Center (EJC) (census 1,800), and the community. We will use the same recruitment strategies and eligibility criteria recently employed to enroll 63 older HIV-infected adults into a cross-sectional exercise study (GET Fit, H-25827). Sixty eligible participants will be will be stratified by gender and AIDS history into one of four strata: (1) Male, history of AIDS ; (2) Male, no history of AIDS; (3) Female, history of AIDS; (4) Female, no history of AIDS. Available subjects in each stratum will be assigned at random 1:1 to either the experimental group or the stretching control group, using the closed envelope method. Targets per training phase for the aerobic training group are expressed as percent heart rate reserve (HRR). The control group will complete 20 weeks of a supervised center-based stretching program. The study phases are listed here: Phase 1- Screening testing and randomization. Phase 2- Baseline outcomes testing. Phase 3- aerobic base training (4 weeks), goal end is 30 minutes at 60-70% HRR. Phase 4- endurance training (12 weeks), goal is 45 minutes at 70-85% HRR Phase 5- follow-up testing #1 Phase 6- short-term sprint interval training (4 weeks), goal is 6 sets of 30 second intervals at 90+% HRR with 4-6 minutes recovery. Phase 7- follow-up testing #2

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目及研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。该提案的目的是研究衰老影响艾滋病毒感染成人的身体功能和有氧能力的机制，并确定有氧运动训练是否可以改善功能和心血管功能。结果将用于设计和测试针对感染艾滋病毒的老年人的具体运动干预措施。我们的长期目标是制定有效的预防和治疗策略，以改善和保持这一快速增长的老年患者群体的功能。具体目标：确定 20 周有氧运动训练（16 周耐力训练加 4 周短冲刺间歇训练）对老年 HIV 成人骨骼肌氧化功能和有氧能力的影响。我们将对 60 名 HIV 受试者进行一项随机试验 = 55 年。将在治疗组（N=30）和对照组（N=30）中在基线、16周和20周评估以下参数： a）有氧能力（VO2peak）； b) 功能性能； c) 骨骼肌氧化功能（细胞色素 C 氧化酶（COX）和琥珀酸脱氢酶（SDH）；d) 骨骼肌线粒体形态（电子显微镜）和 mtDNA（通过 PCR 检测 mt/核 DNA 比率）； e) 去脂体重（DEXA；CT 扫描）； f) 骨骼肌组织学（HIV 相关肌病）。假设 1. 骨骼肌氧化功能障碍是老年 HIV 成人有氧能力降低和功能限制的机制因素。假设 2. 与久坐的老年 HIV 对照相比，有氧运动训练将改善老年 HIV 受试者的肌肉氧化功能障碍以及相关的功能限制和有氧能力降低；然而，改善可能会受到肌肉减少症和艾滋病毒相关肌病的程度的限制。设计/程序：这项随机试验比较了 55 岁 HIV 感染成人中 20 周有氧运动训练与拉伸控制的效果。结果包括生理、功能、肌肉、代谢和身体成分测量，这些测量将在基线、16 周和 20 周时进行评估。所有受试者均感染 HIV，并接受稳定的高效抗逆转录病毒治疗 (HAART)。大多数志愿者将从巴尔的摩退伍军人医疗中心 (VAMC)（人口普查 620）的 ID 诊所、马里兰大学成人艾滋病毒诊所、伊芙琳乔丹中心 (EJC)（人口普查 1,800）和社区招募。我们将使用最近用于招募 63 名老年 HIV 感染者参加横断面运动研究 (GET Fit, H-25827) 的相同招募策略和资格标准。六十名符合资格的参与者将根据性别和艾滋病史分为四个阶层之一：（1）男性，有艾滋病史；（2）男性，无艾滋病史； (3)女性，有艾滋病史； (4)女性，无艾滋病史。每个层中的可用受试者将使用封闭信封方法以 1:1 的比例随机分配到实验组或拉伸对照组。有氧训练组每个训练阶段的目标以心率储备百分比 (HRR) 表示。对照组将完成为期 20 周的监督中心拉伸计划。此处列出了研究阶段：第 1 阶段 - 筛选测试和随机化。第 2 阶段 - 基线结果测试。第 3 阶段 - 有氧基础训练（4 周），目标结束时间为 30 分钟，HRR 为 60-70%。第 4 阶段 - 耐力训练（12 周），目标是在 70-85% HRR 下进行 45 分钟第 5 阶段 - 后续测试 #1 第 6 阶段 - 短期冲刺间歇训练（4 周），目标是 6 组，每组 30 秒间隔时间为 90+% HRR，恢复时间为 4-6 分钟。第 7 阶段 - 后续测试 #2