FITNESS AND LONGEVITY IN EXERCISE: FLEX

运动中的健身和长寿：FLEX

基本信息

批准号：
7718097
负责人：
KRISANN K OURSLER
金额：
$ 0.41万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-03-01 至 2009-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7718097
关键词：
Acquired Immunodeficiency Syndrome Activities of Daily Living Adult Aerobic Aerobic Exercise Affect Aging Baltimore Body Composition Cardiovascular system Censuses Clinic Communities Computer Retrieval of Information on Scientific Projects Database Control Groups Cytochromes DNA Elderly Electron Microscopy Eligibility Determination Enrollment Exercise Female Functional disorder Funding Gender Goals Grant HIV Heart Rate Highly Active Antiretroviral Therapy Histology Institution Intervention Jordan Longevity Maryland Measures Medical center Metabolic Methods Mitochondria Morphology Muscle Myopathy Nuclear Outcome Oxidases Performance Phase Physical Function Physiological Polymerase Chain Reaction Population Prevention Procedures Randomized Randomized Controlled Clinical Trials Recording of previous events Recovery Recruitment Activity Research Research Personnel Resources Screening procedure Skeletal Muscle Source Stretching Succinate Dehydrogenase Testing Training United States National Institutes of Health Universities Week X-Ray Computed Tomography base design eligible participant fitness follow-up improved male older patient programs sarcopenia sedentary trial comparing volunteer

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this proposal is to investigate the mechanisms by which aging affects physical functioning and aerobic capacity in HIV-infected adults, and to determine if aerobic exercise training improves functional and cardiovascular performance. Results will be used to design and test specific exercise interventions for older adults infected with HIV. Our long-term objective is to devise effective prevention and treatment strategies that will improve and preserve function in this rapidly growing older patient population. Specific Aim: To determine the effect of 20 week aerobic exercise training (16 weeks endurance training plus 4 weeks short-sprint interval training) on skeletal muscle oxidative function and aerobic capacity in older HIV adults. We will conduct a randomized trial of 60 HIV subjects = 55 years. The following parameters will be assessed at baseline,16 weeks, and 20 weeks in the treatment group (N=30) and control group (N=30): a) aerobic capacity (VO2peak); b) functional performance; c) skeletal muscle oxidative function (Cytochrome C oxidase (COX) and succinate dehydrogenase (SDH); d) skeletal muscle mitochondrial morphology (electron microscopy) and mtDNA (mt/nuclear DNA ratio by PCR); e) lean body mass (DEXA;CT scan); f) skeletal muscle histology (HIV-related myopathy). Hypothesis 1. Skeletal muscle oxidative dysfunction is a mechanistic factor underlying reduced aerobic capacity and functional limitations in older HIV adults. Hypothesis 2. Aerobic exercise training will improve muscle oxidative dysfunction, and the associated functional limitations and reduced aerobic capacity in older HIV subjects compared to sedentary older HIV controls; however, improvements may be limited by the extent of sarcopenia and HIV-related myopathy. Design/procedure: This randomized trial compares the effect of 20-weeks aerobic exercise training versus stretching control in HIV-infected adults = 55 years. Outcomes include physiologic, functional, muscular, metabolic, and body composition measures which will be assessed at baseline, 16 weeks, and 20 weeks. All subjects will be HIV-infected and on stable highly active antiretroviral therapy (HAART). The majority of the volunteers will be recruited from the ID Clinic at the Baltimore VA Medical Center (VAMC)(census 620) and the University of Maryland adult HIV clinic, Evelyn Jordan Center (EJC) (census 1,800), and the community. We will use the same recruitment strategies and eligibility criteria recently employed to enroll 63 older HIV-infected adults into a cross-sectional exercise study (GET Fit, H-25827). Sixty eligible participants will be will be stratified by gender and AIDS history into one of four strata: (1) Male, history of AIDS ; (2) Male, no history of AIDS; (3) Female, history of AIDS; (4) Female, no history of AIDS. Available subjects in each stratum will be assigned at random 1:1 to either the experimental group or the stretching control group, using the closed envelope method. Targets per training phase for the aerobic training group are expressed as percent heart rate reserve (HRR). The control group will complete 20 weeks of a supervised center-based stretching program. The study phases are listed here: Phase 1- Screening testing and randomization. Phase 2- Baseline outcomes testing. Phase 3- aerobic base training (4 weeks), goal end is 30 minutes at 60-70% HRR. Phase 4- endurance training (12 weeks), goal is 45 minutes at 70-85% HRR Phase 5- follow-up testing #1 Phase 6- short-term sprint interval training (4 weeks), goal is 6 sets of 30 second intervals at 90+% HRR with 4-6 minutes recovery. Phase 7- follow-up testing #2

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。该提案的目的是研究衰老影响HIV感染的成年人的身体功能和有氧能力的机制，并确定有氧运动训练是否改善了功能性和心血管表现。结果将用于针对感染HIV的老年人设计和测试特定的运动干预措施。我们的长期目标是制定有效的预防和治疗策略，这些策略将在这个迅速增长的老年患者人群中改善和保留功能。具体目的：确定20周有氧运动训练（16周耐力训练以及4周的短期间隔训练）对老年HIV成年人骨骼肌氧化功能和有氧运动能力的影响。我们将对60名HIV受试者进行随机试验= 55年。在治疗组（n = 30）和对照组（n = 30）的基线，16周和20周时，将评估以下参数：a）有氧能力（vo2peak）； b）功能性能； c）骨骼肌氧化功能（细胞色素c氧化酶（COX）和琥珀酸脱氢酶（SDH）； d）骨骼肌线粒体形态（电子显微镜）和mtDNA（MT/核DNA比率）； e）瘦体重（DEXA； CT扫描）； F）骨骼肌组织学（与HIV相关的肌病）。假设1。骨骼肌氧化功能障碍是降低有氧运动能力和功能限制的机械因素。假设2。有氧运动训练将改善肌肉氧化功能障碍，以及与久坐的老年HIV对照相比，老年HIV受试者的相关功能局限性和有氧运动能力降低；但是，改善可能受到肌肉减少症和与HIV相关的肌病的限制。设计/程序：这项随机试验比较了20周有氧运动训练与HIV感染的成年人= 55岁的伸展控制的效果。结果包括生理，功能，肌肉，代谢和身体组成测量，将在基线，16周和20周时评估。所有受试者将受到HIV感染，并在稳定的高度活性抗逆转录病毒疗法（HAART）上。大多数志愿者将是从巴尔的摩VA医疗中心（VAMC）（人口普查620）的ID诊所和马里兰大学成人艾滋病毒诊所（EJC）（EJC）（人口普查1,800）和社区招募的。我们将使用最近采用的相同招聘策略和资格标准将63名年龄较大的艾滋病毒感染的成年人纳入横断面运动研究（Get Fit，H-25827）。六十名合格的参与者将通过性别和艾滋病历史分为四个阶层之一：（1）男性，艾滋病历史；（2）男性，没有艾滋病史；（3）女性，艾滋病史；（4）女性，没有艾滋病史。每个层中的可用受试者将使用封闭的包络方法以随机的1：1分配给实验组或拉伸对照组。有氧训练组的每个训练阶段目标表示为心率储备百分比（HRR）。对照组将完成20周监督的基于中心的伸展计划。研究阶段在此处列出：1阶段筛选测试和随机分组。第2阶段基线结果测试。第3阶段 - 有氧基础训练（4周），目标末端为30分钟，为60-70％HRR。第4阶段 - 耐力训练（12周），目标为45分钟，在70-85％HRR阶段5-随访测试＃1第6阶段6-短期SPRINT间隔训练（4周），目标为6套30秒间隔，在90+％HRR时，恢复了4-6分钟。第7阶段 - 后续测试＃2