Slippery Nanoemulsion-Infused Polymer Coatings that Prevent Bacterial Fouling and Block Bacterial Virulence

光滑的纳米乳液注入聚合物涂层可防止细菌污染并阻止细菌毒力

• 批准号: 10667161
• 负责人: DAVID M LYNN
• 金额: $ 21.99万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10667161
• 关键词: Address; Adopted; Anti-Bacterial Agents; Antibiotics; Attenuated; Bacteria; Behavior; Clinical; Collaborations; Development; Devices; Drug Design; Economics; Environment; Equipment; Evaluation; Exhibits; Foundations; Future; Goals; Health; Healthcare; Human; Hydrophobicity; Incidence; Industrialization; Infection; Infusion procedures; Intervention; Knowledge; Lead; Life; Liquid substance; Methods; Modeling; Oils; Organism; Outcome; Pain; Pathogenicity; Patients; Pharmaceutical Preparations; Phase; Polymers; Porosity; Prevention; Property; Research; Research Personnel; Resistance; Rewards; Risk; Staphylococcus aureus; Structure; Surface; Testing; Translations; Virulence; Virulent; Water; Work; antimicrobial; clinical care; clinically relevant; combat; cost; design; economic impact; fabrication; health care settings; human pathogen; implant material; improved; innovation; instrument; microbial; nanoemulsion; novel; novel strategies; pathogenic bacteria; prevent

PROJECT SUMMARY: This R21 project will develop new classes of synthetic liquid-infused surfaces and coatings that prevent bacterial fouling and attenuate bacterial virulence in clinical and healthcare settings. These objectives will be accomplished by the pursuit of two focused and integrated Aims: (1) to explore new designs of slippery nanoemulsion-infused porous surfaces (SNIPS) and characterize the impacts of infused nanoemulsions on antifouling behavior, including the ability to prevent fouling by bacterial pathogens, and (2) to design SNIPS that can host and release active agents and characterize the ability of drug-eluting SNIPS to enhance prevention of surface biofouling and attenuate bacterial load and virulence. Contamination and fouling of surfaces by bacteria pose persistent and costly threats in many industrial, commercial, and clinical healthcare settings. These problems are urgent, and the potential societal and economic impacts of strategies to prevent bacterial fouling and virulence are nearly impossible to overstate. Many strategies have been used to design materials that resist bacterial fouling, but all of them ultimately fail when deployed in real-world scenarios. Fundamentally new approaches to the design of antifouling or `anti- virulence' surfaces that move beyond conventional design strategies are desperately needed and would have substantial impacts on human health. One promising approach to prevent bacterial fouling on surfaces is to exploit the properties of `slippery' liquid-infused porous surfaces. These so-called `SLIPS' have enormous potential in healthcare settings, but are generally passive materials—they can strongly repel bacteria with which they come into contact, but can do little to attenuate the virulent behaviors of organisms in surrounding environments or reduce microbial load. This proposal seeks to advance innovative designs of `drug-eluting' SLIPS that can address this challenge and, thereby, enhance inherent anti-biofouling properties by eluting antimicrobial and anti-virulence agents. The proposed work is based on two broad propositions: (i) that infusion of water-in-oil nanoemulsions, rather than conventional hydrophobic oils, into porous polymer coatings can be used to design `slippery' antifouling materials (`SNIPS') that can host and release bioactive agents, and (ii) that SNIPS containing potent antibiotics and novel anti-virulence agents can reduce bacterial loads and alter bacterial behaviors in ways that enhance inherent anti-biofouling behaviors and expand the practical utility of liquid-infused materials. Our innovative and cross-disciplinary research plan seeks to explore these new ideas and test hypotheses that will create a foundation for the development of new synthetic polymer coatings that can prevent bacterial fouling in practical settings. The scope of the proposed studies embodies novel questions and associated levels of risk that are appropriate for an R21-level study and unites a team of established and actively collaborating investigators to demonstrate and explore the feasibility of this new materials-focused approach.

项目摘要：该 R21 项目将开发新型合成液体注入表面和 在临床和医疗保健环境中防止细菌污染并减弱细菌毒力的涂层。 这些目标将通过追求两个集中且综合的目标来实现：（1）探索新的 光滑纳米乳液注入多孔表面 (SNIPS) 的设计并表征注入的影响 纳米乳液的防污行为，包括防止细菌病原体污染的能力，以及（2） 设计可以容纳和释放活性剂的 SNIPS，并表征药物洗脱 SNIPS 的能力 加强表面生物污染的预防并减弱细菌负荷和毒力。 细菌对表面的污染和结垢对许多工业、 这些问题是紧迫的，并且是潜在的社会和临床医疗保健环境。 防止细菌污染和毒力的策略的经济影响几乎不可能被夸大。 人们使用了许多策略来设计抵抗细菌污染的材料，但它们最终都失败了 当部署在现实世界的场景中时，这是一种全新的防污或“防污”设计方法。 迫切需要超越传统设计策略的毒力表面 对人类健康产生重大影响。 防止表面细菌污染的一种有前途的方法是利用“滑”的特性 这些所谓的“SLIPS”在医疗保健环境中具有巨大的潜力，但 通常是被动材料——它们可以强烈排斥与其接触的细菌，但可以 几乎无法减弱周围环境中生物体的毒性行为或减少微生物负荷。 该提案旨在推进“药物洗脱”SLIPS 的创新设计，以应对这一挑战，并且， 通过洗脱抗菌剂和抗毒剂来增强固有的抗生物污垢特性。 拟议的工作基于两个广泛的主张：（i）注入纳米油乳液中的水， 与传统的疏水性油不同，多孔聚合物涂层可用于设计“光滑” 可以容纳和释放生物活性剂的防污材料（“SNIPS”），以及（ii）含有强效物质的 SNIPS 抗生素和新型抗毒剂可以减少细菌负荷并改变细菌行为 增强固有的抗生物污垢行为并扩大液体注入材料的实用性。 创新和跨学科研究计划旨在探索这些新想法并检验假设 为开发可防止细菌污染的新型合成聚合物涂层奠定了基础 拟议研究的范围体现了新的问题和相关的风险水平。 适合 R21 级别的研究，并组成一个由成熟且积极合作的团队组成 研究人员展示并探索这种以新材料为中心的方法的可行性。