High-Speed High-Sensitivity Detector for X-ray Micro-Crystallography at GM/CA@APS

GM/CA@APS 用于 X 射线微晶体学的高速高灵敏度探测器

• 批准号: 10632971
• 负责人: ROBERT F. FISCHETTI
• 金额: $ 200万
• 依托单位: UCHICAGO ARGONNE, LLC
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632971
• 关键词: 10 year old; Complex; Coupled; Crystallography; Data; Data Collection; Detection; Dose; Funding; Generations; Optics; Phase; Photons; Preparation; Research Personnel; Resolution; Rod; Roentgen Rays; Sampling; Shapes; Software Tools; Source; Speed; United States National Institutes of Health; Viscosity; Vision; beamline; detector; lens; next generation; quantum; structural biology; tool

Project Summary GM/CA @ APS operates two highly successful undulator beamlines as a national user facility for macromolecular crystallography at the Advanced Photon Source (APS). The GM/CA group develops and operates world-leading beamlines in micro-crystallography, offering user-selectable “min-beam” sizes down to 5 m for all samples. Giving users the ability to change beam size rapidly, reliably, and on-demand without staff involvement to optimize the beam size for a given project has led to a sweeping change in experimental practice. The hardware for micro-crystallography is coupled with sophisticated software tools in the JBluIce user interface. Essential tools include rastering to find micro- crystals in complex mounts or to identify the best diffracting regions of larger inhomogeneous samples, helical data collection to distribute dose along rod-shaped crystals and serial data collection from viscous jets or fixed targets. Consequently, the GM/CA beamlines are oversubscribed due to the high demand by a growing number of investigators with challenging NIH-funded structural projects. We are about to embark on an exciting new phase. Our vision is to provide the highest resolution data achievable from challenging problems in structural biology that tend to yield tiny, weakly diffracting crystals. The APS will shut down in April of 2023. The storage ring will be upgraded (APS-U) with a 4th generation multi-bend achromat storage ring, increasing the X-ray brightness by two to three orders of magnitude depending on the X-ray energy. In preparation for the APS-U start-up in April 2024, new focusing optics were installed on 23ID-D. The new mirrors will focus the APS-U source to a beam as small as ~5-microns, and new Compound Refractive Lenses will provide an intense ~1-micron beam. Combining the APS-U source and the new optics is a game-changing opportunity for structural biology. However, beamline 23ID-D has a Pilatus3 6M detector that will be ten years old when the APS-U starts up and must be replaced. To realize our vision, we propose to purchase an Eiger2 XE 16M CdTe detector from Dectris, AG, a next-generation high-speed, high count rate detector with high quantum efficiency for the detection of X-rays with energies from 6 – 35 keV.

项目概要 GM/CA @ APS 运营着两条非常成功的波荡器光束线，作为国家用户设施 GM/CA 小组开发了高级光子源 (APS) 的大分子晶体学。 并在微晶体学领域运营世界领先的光束线，提供用户可选的“最小光束” 所有样品的尺寸小至 5 µm，使用户能够快速、可靠地改变光束尺寸。 无需工作人员参与即可按需优化给定项目的光束尺寸，这导致了 微晶体学的硬件与实验实践发生了巨大的变化。 JBluIce 用户界面中的先进软件工具 基本工具包括用于查找微观特征的光栅化。 复杂安装中的晶体或识别较大不均匀样品的最佳衍射区域， 螺旋数据收集沿着棒状晶体分布剂量和串行数据收集 检查粘性射流或固定目标时，由于高，GM/CA 光束线被超额认购。 越来越多的研究人员对具有挑战性的 NIH 资助的结构项目提出了需求。 我们即将进入一个令人兴奋的新阶段，我们的愿景是提供最高分辨率的数据。 可以通过结构生物学中具有挑战性的问题来实现，这些问题往往会产生微小、弱的衍射 APS将于2023年4月关闭。存储环将升级为第4个（APS-U）。 一代多弯消色差存储环，将X射线亮度提高两到三个数量级 星等取决于 X 射线能量 为 2024 年 4 月 APS-U 启动做准备，新的。 光学器件安装在 23ID-D 上，新的聚焦镜将 APS-U 光源聚焦为光束。 小至约 5 微米，新型复合折射透镜将提供强烈的约 1 微米光束。 APS-U 光源和新光学器件的结合为结构生物学带来了改变游戏规则的机会。 然而，Beamline 23ID-D 有一个 Pilatus3 6M 探测器，当 APS-U 启动时，该探测器已经有十年的历史了 为了实现我们的愿景，我们建议购买 Eiger2 XE 16M CdTe。 Dectris, AG 的探测器，具有高量子能力的下一代高速、高计数率探测器 检测能量为 6 – 35 keV 的 X 射线的效率。