A PILOT STUDY TO EVALUATE NUCLEIC ACID AMPLIFICATION TESTS TO PREDICT TB RELAPSE

评估核酸扩增测试预测结核病复发的试点研究

• 批准号: 7718708
• 负责人: MARC H WEINER
• 金额: $ 0.02万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7718708
• 关键词: Blinded; Centers for Disease Control and Prevention (U.S.); Computer Retrieval of Information on Scientific Projects Database; DNA; Enrollment; Evaluation; Funding; Grant; Informed Consent; Institution; Leukocytes; Measures; Messenger RNA; Nucleic Acid Amplification Tests; Nucleic Acids; Pilot Projects; Relapse; Research; Research Design; Research Personnel; Resources; Ribosomal RNA; Source; Specimen; Sputum; Testing; Time; Tuberculosis; United States National Institutes of Health; United States Public Health Service; Writing; inclusion criteria; treatment duration; treatment trial; tuberculosis treatment

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. OBJECTIVE: The aim of this study is to determine the utility of different nucleic acid amplification and broth culture tests obtained early in treatment and in the post-treatment periods to predict subsequent relapse of tuberculosis. Bacillary burden and MTB viability will be measured: in sputa by quantitative rRNA, qualitative (MTD Gen-Probe) rRNA, mRNA; 'time to positivity' in broth cultures; and in circulating leukocytes ("burry coat" specimens) by MTB DNA. RESEARCH PLAN: The study design is a blinded retrospective evaluation of specimens obtained in the course of two multi-center TB treatment trials. This is a CDC sponsored study. Inclusion criteria are (1) enrollment in TBTC / USPHS Trials 23 or 24, and (2) provision of written informed consent.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目的：这项研究的目的是确定在治疗早期和处理后期获得的不同核酸扩增和肉汤培养试验的实用性，以预测随后的结核病复发。 将测量细菌负担和MTB生存力：在痰液中，定量rRNA，定性（MTD Gen-Probe）rRNA，mRNA；肉汤文化中的“积极性”；以及MTB DNA循环的白细胞（“ Burry Coat”标本）。 研究计划：研究设计是对两项多中心结核病治疗试验中获得的标本的盲目回顾性评估。 这是CDC赞助的研究。 纳入标准是（1）入学TBTC / USPHS试验23或24，以及（2）提供书面知情同意书。