Functional characters of microRNAs in T lymphocytes

T淋巴细胞中microRNA的功能特征

• 批准号: 7624610
• 负责人: MARIANTHI KIRIAKIDOU
• 金额: $ 12.02万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-15 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7624610
• 关键词: Address; Apoptosis; Base Pairing; Biology; Cell Proliferation; Cell physiology; Clinical; Development; Elements; Gene Expression; Gene Expression Regulation; Gene Targeting; Genes; Goals; Hematopoietic; Human; Immunology; Institution; Internal Medicine; Knowledge; Lymphocyte; Maintenance; Messenger RNA; MicroRNAs; Molecular Biology; Mus; Organism; Pathway interactions; Pattern; Pear; Physicians; Play; Research Personnel; Research Training; Resources; Rheumatology; Role; Scientist; Staging; Steroid biosynthesis; T-Cell Development; T-Lymphocyte; Time; Training; Validation; career; novel; research facility

DESCRIPTION (provided by applicant): The dual goals of this proposal are to investigate the function of microRNAs in hematopoietic/T cell development and to establish the applicant as an independent investigator. MicroRNAs represent a novel class of small regulatory RNAs. miRNAs control gene expression by base pairing with miRNA-recognition elements found in their mRNA targets. The effects of miRNAs in gene expression regulation are pleiotropic, miRNAs control cellular proliferation, apoptosis and development, although many of the genes that mammalian miRNAs regulate are unknown. The role of miRNAs in T lymphocytes and their development is completely unknown. Studies to address the function of miRNAs in hematopoietic/T cell development promise to unravel novel pathways that control hematopoietic/lymphocytic development and at the same time enhance our understanding of the function of miRNAs. In Aim #1, mRNA targets for mammalian miRNAs will be identified. An experimental-computational approach is presented along with strategies for validation of miRNA targets. In Aim #2, the function of miRNAs in murine hematopoietic/T cell development will be investigated. The applicant has spent the last several years for a career as a physician-scientist, through her prior research training in the molecular biology of steroidogenesis and clinical training in Internal Medicine and Rheumatology. Drs. Warren Pear, Zissimos Mourelatos and Philip Cohen, recognized for their expertise in the fields of T cell development and Immunology (Drs. Pear and Cohen) and microRNA biology (Dr. Mourelatos), will sponsor the candidate's scientific development. The quality of the research facilities and the diversity of the resources available at the Sponsoring Institution in combination with the intellectual and academic strength of the sponsors, provide an ideal setting for the conduction of the proposed studies.

描述（由申请人提供）：该提案的双重目标是研究microRNA在造血/T细胞发育中的功能，并确定申请人为独立研究者。 microRNA代表了一类新型的小型RNA。 miRNA通过基碱与miRNA识别元件配对的mRNA识别元件在其mRNA靶标中控制基因表达。 miRNA在基因表达调节中的作用是多效性，miRNA控制细胞增殖，凋亡和发育，尽管哺乳动物miRNAS调节的许多基因尚不清楚。 miRNA在T淋巴细胞中的作用及其发育是完全未知的。解决造血/T细胞发育中miRNA的功能的研究有望揭示控制造血/淋巴细胞发育的新途径，同时又增强了我们对miRNA功能的理解。在AIM＃1中，将确定哺乳动物miRNA的mRNA靶标。提出了一种实验计算方法以及验证miRNA靶标的策略。在AIM＃2中，将研究miRNA在鼠造血/T细胞发育中的功能。申请人在过去的几年中一直在医师科学家的职业生涯中度过，她先前在内科和风湿病学的类固醇生成和临床培训方面进行了研究培训。博士。沃伦·皮尔（Warren Pear），Zissimos Mourelatos和Philip Cohen因其在T细胞开发和免疫学领域的专业知识（Pear和Cohen博士）和MicroRNA Biology（Morelatos博士）而闻名，他们将赞助候选人的科学发展。研究设施的质量以及赞助机构可用的资源与赞助商的智力和学术实力相结合的多样性为进行拟议的研究提供了理想的环境。

项目成果

Clarifying mammalian RISC assembly in vitro.

澄清哺乳动物 RISC 体外组装。

• DOI: 10.1186/1471-2199-12-19
• 发表时间: 2011
• 期刊: BMC molecular biology
• 作者: Tan,GraceS;Garchow,BarryG;Liu,Xuhang;Metzler,David;Kiriakidou,Marianthi
• 通讯作者: Kiriakidou,Marianthi