Virus Detection Using Microarrays and Characterization of a Novel Cardiovirus

使用微阵列进行病毒检测和新型心脏病毒的表征

基本信息

批准号：
7680171
负责人：
Charles Yen Chiu
金额：
$ 12.54万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2011-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7680171
关键词：
Acute Adult Adult Respiratory Distress Syndrome Age Ambulatory Care Facilities Animal Model Antibody Formation Antiviral Agents Australia Award Biological Assay Biomedical Research Biophysics Biopsy Blood Banks Brain British Columbia Bronchoalveolar Lavage Fluid California Canada Cardiomyopathies Cardiovirus Cell Culture Techniques Cell Line Cells Child Childhood Chills Clinical Clinical Research Collaborations Communicable Diseases Communities Critical Illness DNA Microarray Chip Demyelinating Diseases Development Diagnosis Diagnostic Diagnostic tests Disease Doctor of Philosophy Economics Encephalitis Endothelium Etiology Feces Fever Fluorescent Antibody Technique Generations Genome Goals Gold Guide prevention Health Services Healthcare Human Individual Infection Influenza Life Liquid substance Mouse Strains Multiple Sclerosis Mus Myocarditis Neurologic Northern Blotting Organ Oropharyngeal Pan Genus Patients Pharyngeal structure Plasmids Prevalence Production Public Health RNA Research Research Personnel Respiratory Tract Infections Reverse Transcriptase Polymerase Chain Reaction Role Run-On Assays Sampling San Francisco Satellite Viruses Screening procedure Seasons Sensitivity and Specificity Serological Seroprevalences Serum Shotgun Sequencing Societies Swab TMEV Tissues Universities Upper respiratory tract Vaccines Viral Virus Virus Diseases Work base brain tissue care burden clinical practice flu high throughput screening human disease innovation mouse model novel novel virus pathogen programs promoter research study respiratory respiratory virus response restriction enzyme tool viral DNA viral detection

项目摘要

DESCRIPTION (provided by applicant): This is an application for a K08 award for Dr. Charles Chiu, a fellow in adult infectious diseases at the University of California, San Francisco and a 3rd year postdoctoral research fellow with a PhD in biophysics. UCSF is a leading academic biomedical research center with a strong clinical and research community. The work proposed here will help establish the candidate as an independent translational investigator in infectious diseases. The overall goal of the candidate's proposed research is to use an innovative pan-viral DNA microarray (Virochip) to detect and discover viruses associated with human respiratory infection. Acute respiratory tract infection imposes a major economic and health care burden on society. Viruses are associated with both lower and upper respiratory tract illnesses, yet a specific pathogen cannot be identified in 30-60% of cases. The candidate has demonstrated in preliminary experiments that the Virochip is comparable in sensitivity and specificity to that of routine clinical diagnostic tests and that the Virochip is capable of detecting viruses that elude all currently available diagnostic assays. For the proposed research, the candidate will use the Virochip to retrospectively analyze samples of bronchoalveolar lavage fluid from 33 patients with acute respiratory distress syndrome (Aim 1a) and prospectively analyze respiratory secretions from 15-20 critically ill patients in the ICU (Aim 1b) for the presence of viruses. To determine a potential causal relationship between a detected virus and the respiratory illness, the candidate will assess specific antibody responses and look for the virus in other bodily fluids and tissues. To search for novel viruses associated with respiratory illness, the candidate will also use the Virochip to screen culture-negative and viral PCR-negative respiratory samples collected by the California Department of Health Services (Aim 2). The candidate expects that potentially novel viruses will be discovered from this screen, and he will then proceed to sequence and develop specific serological or PCR-based assays for these viruses. Relevance: Understanding viral etiologies of critical respiratory illness may help guide prevention, diagnosis, and treatment of these life-threatening infections. Discovery of a novel respiratory virus would have significant implications on clinical practice and public health and may stimulate development of new antiviral drugs and vaccines.

描述（由申请人提供）：这是 Charles Chiu 博士的 K08 奖申请，他是加州大学旧金山分校成人传染病研究员，也是生物物理学博士学位的三年级博士后研究员。加州大学旧金山分校是领先的学术生物医学研究中心，拥有强大的临床和研究社区。这里提出的工作将有助于使候选人成为传染病领域的独立转化研究者。候选人提出的研究的总体目标是使用创新的泛病毒 DNA 微阵列 (Virochip) 来检测和发现与人类呼吸道感染相关的病毒。急性呼吸道感染给社会带来重大的经济和医疗负担。病毒与下呼吸道和上呼吸道疾病有关，但无法识别特定的病原体 30-60% 的情况下。候选人在初步实验中证明了 Virochip 是 Virochip 的灵敏度和特异性与常规临床诊断测试相当，并且能够检测出所有目前可用的诊断检测方法无法检测到的病毒。在拟议的研究中，候选人将使用 Virochip 回顾性分析 33 名急性呼吸窘迫综合征患者的支气管肺泡灌洗液样本（目标 1a），并前瞻性分析 ICU 中 15-20 名重症患者的呼吸道分泌物（目标 1b）对于病毒的存在。为了确定检测到的病毒与呼吸道疾病之间的潜在因果关系，候选人将评估特定抗体反应并在其他体液和组织中寻找病毒。为了寻找与呼吸道疾病相关的新型病毒，候选人还将使用 Virochip 筛选由加州卫生服务部收集的培养阴性和病毒 PCR 阴性呼吸道样本（目标 2）。候选人预计将从该筛选中发现潜在的新型病毒，然后他将对这些病毒进行测序并开发特定的血清学或基于 PCR 的检测方法。相关性：了解危重呼吸道疾病的病毒病因可能有助于指导这些危及生命的感染的预防、诊断和治疗。新型呼吸道病毒的发现将对临床实践和公共卫生产生重大影响，并可能刺激新的抗病毒药物和疫苗的开发。