Gene Expression by Moraxella catarrhalis in vivo

卡他莫拉菌体内基因表达

• 批准号: 7685382
• 负责人: Todd C Hoopman
• 金额: $ 10.8万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7685382
• 关键词: Address; Adult; Affect; Animal Model; Antibodies; Antigens; Area; Award; Bacteria; Bacterial Proteins; Biological Models; Child; Chinchilla (genus); Chronic Obstructive Airway Disease; Clinical; Commit; DNA Microarray Chip; Development; Development Plans; Disease; Ensure; Environment; Evaluation; Faculty; Fostering; Foundations; Gene Expression; Genes; Goals; Growth; Human; Infant; Institution; Instruction; K-Series Research Career Programs; Laboratories; Light; Lower respiratory tract structure; Mentors; Microarray Analysis; Microbial Biofilms; Microbiology; Modeling; Molecular; Moraxella (Branhamella) catarrhalis; Mutation; Nasopharynx; Organism; Otitis Media; Physicians; Process; Proteins; Research; Research Personnel; Resources; Respiratory Tract Diseases; Scientific Advances and Accomplishments; Scientist; Solid; Study models; Surface; Techniques; Time; Training; Vaccinated; Vaccines; Work; career development; design; ear infection; experience; in vivo; mutant; pathogen; skills training; time use

DESCRIPTION (provided by applicant): The global objective of this Mentored Clinical Scientist Research Career Development Award is to facilitate the development of the Candidate as an academic physician-scientist and to ensure his transition to independence. In light of these goals, the Candidate and his Mentor have designed an integrated plan that includes a strong foundation of didactic instruction in the field of molecular microbiology, combined with an intensive research component. The didactic component of his training will be directed by the experienced faculty at UT Southwestern, and will help to establish and foster a solid understanding of the important scientific principles and thought processes necessary for independence as an investigator. The Candidate will also receive training in cutting-edge and advanced scientific techniques in the laboratory of the Mentor and the Department of Microbiology. The Candidate's research progress and career development will be assessed and guided by a Mentoring Committee composed of established and successful physician-scientists at the Candidate's institution. The proposed award will provide the Candidate with the resources, training, and skills necessary for successful development into an independent physician-scientist. His career development plan fully addresses areas that are key to his successful growth, and this institution has committed to protect 80% of his time for these activities. The Candidate has successfully implemented a model for the study of nasopharyngeal colonization by Moraxella catarrhalis in the chinchilla. For the first time, the use of this model system will allow for the study of M. catarrhalis gene products expressed in vivo. In the first Specific Aim, DNA microarray technology will be utilized to identify M. catarrhalis genes that are induced or up-regulated during growth in the chinchilla nasopharynx. Mutant analysis will be used in the second Specific Aim to determine which of these gene products is essential for survival of this organism in the chinchilla nasopharynx. Finally, in the third Specific Aim, selected M. catarrhalis proteins which are determined to be exposed on the surface of this bacterium will be used as experimental immunogens to evaluate their ability to vaccinate against nasopharyngeal colonization in this animal model. Moraxella catarrhalis is a bacterial pathogen that is an important cause of respiratory tract disease in adults with certain preexisting conditions (i.e., chronic obstructive pulmonary disease) and in babies and very young children (i.e., ear infections). Information obtained from this research will identify components of this bacterium that have the potential to be developed into a vaccine to protect against M. catarrhalis disease.

描述（由申请人提供）：这一指导临床科学家研究职业发展奖的全球目标是促进候选人作为学术医师 - 科学家的发展，并确保他向独立过渡。鉴于这些目标，候选人及其导师设计了一个综合计划，其中包括分子微生物学领域的教学指导的强大基础，并结合了强化的研究组成部分。他的培训的教学成分将由UT西南大学的经验丰富的教师指导，并将有助于建立和促进对独立作为研究者所必需的重要科学原则和思维过程的扎实理解。候选人还将接受导师和微生物学系实验室的尖端和先进科学技术的培训。候选人的研究进度和职业发展将由由候选人机构的既定且成功的医师科学家组成的指导委员会进行评估和指导。拟议的奖项将为候选人提供成功发展为独立医师科学家所必需的资源，培训和技能。他的职业发展计划完全解决了他成功发展的关键领域，该机构致力于保护他的80％的时间从事这些活动。该候选人成功实施了一个模型，用于研究麦芽孢菌的鼻咽定植。该模型系统的使用首次将允许研究在体内表达的Cartarrhalis基因产物。在第一个特定目的中，将利用DNA微阵列技术来识别龙骨鼻咽生长期间被诱导或上调的Cartarrhalis基因。突变分析将在第二个特定目的中使用，以确定这些基因产物中的哪种对于该生物在龙猫Nasopharynx中的生存至关重要。最后，在第三个特定目的中，确定在该细菌表面暴露的选定的卡他律蛋白质蛋白将用作实验免疫原子，以评估其在该动物模型中针对鼻咽静脉结肠化疫苗接种的能力。摩拉氏菌Catarrhalis是一种细菌病原体，是患有某些先前存在疾病（即慢性阻塞性肺疾病）以及婴儿和非常小的孩子（即耳部感染）的成年人中呼吸道疾病的重要原因。从这项研究中获得的信息将确定该细菌的成分，这些细菌有可能发展为疫苗以防止卡他氏菌疾病。