Integration of resources and studies to elucidate neuropeptide signaling

整合资源和研究以阐明神经肽信号传导

基本信息

批准号：
7762955
负责人：
SANDRA L RODRIGUEZ ZAS
金额：
$ 19.01万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7762955
关键词：
Address Animal Model Bees Behavior Bioinformatics Biological Process Brain Characteristics Classification Code Communities Complement Complex Computer Simulation Data Databases Development Diagnosis Diagnostic Disease Drug Addiction Drug abuse Genes Goals Grant Honey Human Joints Knowledge Label Lead Learning Link Mass Spectrum Analysis Mediating Mining Modeling Mus National Institute of Drug Abuse Neurons Neuropeptide Receptor Neuropeptides Neurosciences Neurosciences Research Ontology Outcome Peptide Signal Sequences Peptides Pharmaceutical Preparations Phase Post-Translational Protein Processing Prevention Proteomics Publishing Rattus Research Research Infrastructure Resolution Resources Role Signal Transduction Simulate Stimulus Substance abuse problem System Systems Biology Techniques Transcript Validation Work abstracting addiction crosslink drug of abuse extracellular improved insight intercellular communication prognostic prohormone public health relevance ranpirnase repository research study response tool transcriptomics

Address Animal Model Bees Behavior Bioinformatics Biological Process Brain Characteristics Classification Code Communities Complement Complex Computer Simulation Data Databases Development Diagnosis Diagnostic Disease Drug Addiction Drug abuse Genes Goals Grant Honey Human Joints Knowledge Label Lead Learning Link Mass Spectrum Analysis Mediating Mining Modeling Mus National Institute of Drug Abuse Neurons Neuropeptide Receptor Neuropeptides Neurosciences Neurosciences Research Ontology Outcome Peptide Signal Sequences Peptides Pharmaceutical Preparations Phase Post-Translational Protein Processing Prevention Proteomics Publishing Rattus Research Research Infrastructure Resolution Resources Role Signal Transduction Simulate Stimulus Substance abuse problem System Systems Biology Techniques Transcript Validation Work abstracting addiction crosslink drug of abuse extracellular improved insight intercellular communication prognostic prohormone public health relevance ranpirnase repository research study response tool transcriptomics

展开

项目摘要

DESCRIPTION (provided by applicant): Integration of resources and studies to elucidate neuropeptide signaling Abstract Elucidating the mechanisms governing drug addiction requires knowledge of the role of neuropeptides on the neuronal networks. These signaling peptides mediate responses to environmental stimuli and influence addiction behaviors. Many drugs of abuse directly interact with neuropeptide receptors, and others appear to mediate peptide responses. NIDA has a long tradition of supporting fundamental neuroscience research that has lead to the elucidation of signaling systems and provided critical insights into substance abuse and behavior. Signaling peptides have been studied using a wide range of techniques and model organisms. Multiple complementary neuropeptide repositories and peptidomic and transcriptomic experiments are now available. Even with such information, the challenge remains to gain a complete and systematic understanding of the neuropeptidome and its association with drug escalation and abuse. We propose to address this challenge by developing a public and comprehensive neuropeptide resource much needed by the research community and by collectively analyzing proteomic and transcriptomic experiments to augment the understanding of extracellular signaling peptides both at the fundamental neuroscience as well as the applied substance abuse levels. To accomplish these objectives, we plan to (Aim 1) integrate complementary peptide repositories and develop tools to assemble and effectively query a comprehensive and public resource of experimental and in silico predictions; mine this resource to (Aim 2) perform secondary and joint analysis of available high proteomic experiments; and (Aim 3) perform integrated analysis of proteomic and transcriptomic experiments. The overarching strategy is to integrate complementary information across databases, experiments and platforms to provide a unique and comprehensive understanding of the dynamic neuropeptide complement. The outcome of this project will be resources, tools and information that will fill critical gaps in the knowledge on intercellular signaling systems and suggest targets for prevention, diagnosis and cure of substance abuse. PUBLIC HEALTH RELEVANCE: Neuropeptides are signaling peptides that both influence and are influenced by drug abuse and complex behaviors. The proposed project strengthens the significant contributions of NIDA to the advancement of neuropeptide and drug abuse research by developing public bioinformatic resources and by integrating and further mining information from experiments already available. Outcomes from the proposed work will advance the identification and characterization of neuropeptide that support the prognostic, diagnostic and treatment of disorders associated with substance abuse.

描述（由申请人提供）：阐明神经肽信号传导的资源和研究的整合抽象阐明了有关药物成瘾的机制，需要了解神经肽在神经元网络中的作用。这些信号肽介导对环境刺激的反应并影响成瘾行为。许多滥用药物直接与神经肽受体相互作用，而另一些药物似乎介导了肽反应。 NIDA具有支持基本神经科学研究的悠久传统，这导致了信号系统的阐明，并为滥用药物和行为提供了重要的见解。已经使用广泛的技术和模型生物研究了信号肽。现在可以使用多种互补的神经肽存储库以及肽组和转录组实验。即使有了这样的信息，挑战仍然是对神经肽组及其与药物升级和滥用的关联的完整而系统的了解。我们建议通过开发研究界急需的公共和全面的神经肽资源，并共同分析蛋白质组学和转录组学实验，以增强对基本神经科学的细胞外信号肽的理解以及应用物质滥用水平的理解。为了实现这些目标，我们计划（AIM 1）整合互补的肽存储库，并开发工具，以组装并有效地查询实验和硅预测中的全面和公共资源；将此资源挖掘为（AIM 2）对可用的高蛋白质组学实验进行次要和联合分析；（目标3）对蛋白质组学和转录组实验进行综合分析。总体策略是整合跨数据库，实验和平台的互补信息，以提供对动态神经肽补体的独特而全面的理解。该项目的结果将是资源，工具和信息，这些资源，工具和信息将填补有关细胞间信号系统知识的关键空白，并提出预防，诊断和治愈药物滥用的目标。公共卫生相关性：神经肽是信号的肽，既影响又受药物滥用和复杂行为的影响。拟议的项目通过开发公众的生物信息学资源，通过已经可用的实验中整合和进一步的采矿信息来增强NIDA对神经肽和药物滥用研究的重要贡献。拟议工作的结果将推动神经肽的识别和表征，这些神经肽支持与药物滥用相关的疾病的预后，诊断和治疗。