NIEHS Center Aquatic Animal Facility Renovation

NIEHS中心水生动物设施改造

• 批准号: 7628865
• 负责人: DAVID Harold PETERING
• 金额: $ 43.02万
• 依托单位: UNIVERSITY OF WISCONSIN MILWAUKEE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7628865
• 关键词: Accident and Emergency department; Adult; Animal Model; Animals; Back; Biological Models; Biomedical Research; Cardiac; Caring; Child; Clinical Investigator; Development; Disease; Embryo; Environmental Health; Fishes; Freezing; Funding; Genetic; Height; Housing; Human Resources; Institutes; Life; Liquid substance; Maintenance; Microscope; Modeling; Monitor; National Institute of Environmental Health Sciences; Neurologic; Nitrogen; Oxygen; Request for Proposals; Research Support; Scientist; Secure; Source; Staging; Sterilization for infection control; Survival Rate; System; Temperature; Time; Toxicology; Universities; Water; Water Supply; Wisconsin; Zebrafish; animal facility; base; cryogenics; developmental disease; developmental toxicology; facility renovation; public health relevance; reproductive; sperm cell; water quality

DESCRIPTION (provided by applicant): The NIEHS Children's Environmental Health Sciences Core Center provides research support to a cadre of scientists who use aquatic animals as model organisms in their biomedical research. With a focus on toxicology and environmental health, the Center has recently moved to emphasize the zebrafish as a prime model in developmental toxicology. Targeted hiring at the University of Wisconsin-Milwaukee and the formation of the inter-institutional Children=s Environmental Health Institute have greatly expanded the Center=s membership and made it necessary to undertake the conversion of the Aquatic Animal Facility (AAF) from a general purpose biomedical aquatic animal facility to one that focuses on the zebrafish and also can provide care for a few other fish species. The objective is establish a unit that supports high survival rates and use of all life stages from the embryo to the adult of these fish species. In this context, the proposal requests funds for remodeling the facility with the following specific aims: (1) To provide a secure, keyless access system for the facility and its internal rooms and emergency power to the facility. (2) To physically remodel the entry space to the fish facility so that fish obtained from exogenous sources (e.g. wild caught or from other animal facilities) are physically separated from in-house fish stocks, thus, reducing the potential for introducing disease into the fish facility stocks, while maintaining the ability to introduce fresh genetic stocks. (3) To install a large capacity deionized water system with an automated injector to augment or replace the dechlorinated water system presently used to supply water to the fish. (4) To acquire and install water quality monitoring systems for each recirculating and flow through rack system for maintenance of water quality. The monitoring systems would monitor in real time the levels of dissolved oxygen, pH salinity, and temperature and alert appropriate personnel when any of the monitored components wnet outside designated parameters. (5) To replace standard lab height benches with lower benches for use with stereo dissecting microscopes. (6) To acquire stereo and inverted microscopes. (7) To obtain a cryogenic system with freezing rate control and liquid nitrogen based storage system for generating and maintaining frozen sperm as back ups for valued zebrafish strains. (8) To purchase a replacement autoclave to provide sterilization capacity for the facility. PUBLIC HEALTH RELEVANCE (provided by applicant): The Aquatic Animal Facility is a unique unit that supports the use of zebrafish by providing scientists with husbandry support, specialized facilities, and staff expertise. The zebrafish is a prime developmental biological model. Its existence has facilitated the assembly of a critical mass of basic and clinical investigators who collaborate and use this model to understand the origins of reproductive and developmental diseases and disorders, particularly those related to cardiac and neurological development.

描述（由申请人提供）：NIEHS儿童环境健康科学核心中心为一群使用水生动物作为生物医学研究中的模型生物的科学家提供了研究支持。该中心侧重于毒理学和环境健康，最近已提出强调斑马鱼作为发育毒理学中的主要模型。在威斯康星大学 - 米尔沃基分校的招聘和机构间儿童的形成= S环境健康研究所的形成大大扩展了中心= S会员资格，并有必要将水生动物设施（AAF）转换为从一般的生物医学生物医学动物设施中，从而将重点放在Zebrafish上，并提供一些其他范围，以提供一些其他物种，并提供其他其他范围。该目标是建立一个支持高存活率和所有生命阶段使用的单位，从胚胎到这些鱼类的成年人。在这种情况下，该提案要求以以下特定目的对设施进行改建：（1）为设施及其内部房间及其内部房间以及设施的紧急功率提供安全，无钥匙的访问系统。 （2）要物理地重塑入口空间，以便从外源性来源获得的鱼（例如，捕获的野生动物或其他动物设施）与内部鱼类的储备物进行了物理分离，因此，降低了将疾病引入鱼类设施库存的潜力，同时维持了引入新鲜遗传库存的能力。 （3）使用自动喷油器安装大容量去离子水系统，以增强或更换目前用于向鱼类供水的脱氯水系统。 （4）为每个循环和流过机架系统的循环并安装水质监测系统，以维持水质。监测系统将实时监视溶解的氧气，pH盐度和温度的水平，并在任何受监视的组件WNET外部指定参数时提醒适当的人员。 （5）用较低的长凳代替标准实验室高度长凳，以与立体声剖析显微镜一起使用。 （6）获取立体声和倒置显微镜。 （7）获得具有冷冻速率控制和基于液氮的储存系统的低温系统，以生成和维持冷冻的精子作为质量斑马鱼菌株的后备。 （8）购买替换高压灭菌器以提供该设施的灭菌能力。 公共卫生相关性（由申请人提供）：水上动物设施是一个独特的单位，通过为科学家提供饲养，专业设施和员工专业知识来支持斑马鱼的使用。斑马鱼是主要的发育生物学模型。它的存在促进了大量基本和临床研究人员的组装，他们合作并使用此模型来理解生殖和发育疾病和疾病的起源，尤其是与心脏和神经科学有关的疾病。