Extracorporeal and Endoscopic SWIR Mapping of Dynamic Muscle Function

动态肌肉功能的体外和内窥镜短波红外映射

• 批准号: 10650439
• 负责人: JUSTUS M ANUMONWO
• 金额: $ 19.5万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10650439
• 关键词: Affect; Air; Anesthesia procedures; Animals; Area; Basic Science; Bladder; Blood; Body Surface; Breathing; Cardiac; Cardiac Myocytes; Catheterization; Clinical; Complex; Coupled; Coupling; Data; Data Set; Dependence; Development; Disease; Dissociation; Dyes; Electrocardiogram; Endoscopes; Endoscopy; Fingerprint; Fluorescence; Future; Goals; Health; Heart; Heart failure; Heterogeneity; Image; Intestines; Label; Light; Lighting; Link; Maps; Mechanics; Methods; Modality; Monitor; Mus; Muscle; Muscle Cells; Muscle Contraction; Muscle Fibers; Muscle function; Muscular Dystrophies; Myocardial; Myocardium; Myopathy; Noise; Optics; Organ; Parkinson Disease; Patients; Pattern; Property; Research; Right atrial structure; Right ventricular structure; Rod; Rodent; Short Waves; Shortwave Infrared Imaging; Signal Transduction; Smooth Muscle Myocytes; Source; Speed; Structure; Structure of jugular vein; System; Technology; Testing; Time; Tissues; Toxic effect; Uterus; Visual; body cavity; body position; clinical application; clinical diagnostics; electrical property; flexibility; heart function; imaging system; in vivo; in vivo imaging; instrument; lens; light intensity; mechanical properties; meter; movie; novel; novel strategies; optical imaging; photonics; spatiotemporal; spectroscopic survey; ultrasound; voltage sensitive dye

PROJECT SUMMARY/ABSTRACT The contraction of muscle cells in organs such as the heart, uterus, urinary bladder and intestine is regulated by and is affecting a complex activity associated with electrical excitation. Our long-term goal is to develop a paradigm-shifting approach for studying the dynamic actions and coupling between electrical and mechanical properties in muscular tissues and organs to better link them to health and diseases such as Parkinson’s disease and heart failure. New short-wave infrared (SWIR; ~1-2.5 µm) light imaging has bands with relatively low blood absorbance and scattering and has been proposed recently for both deep tissue and in vivo imaging. The general objective of this project is to develop an in vivo extracorporeal and endoscopic label-free SWIR approach for mapping patterns of muscular function. Accordingly, label-free multi-spectral datasets recorded simultaneously at optimized SWIR wavelengths will provide novel spectroscopic fingerprints of electro- mechanical actions in muscle tissue. We will use beating hearts of living mice as a platform for developing the technology for high-speed probing at multiple SWIR wavelengths to test the general hypothesis that in vivo intrinsic optical imaging can characterize simultaneously the distinct dynamic spatiotemporal patterns of electrical and mechanical muscular actions. Our Specific Aims are: Aim 1: To demonstrate that intrinsic multi-spectral SWIR light imaging can be used for extracorporeal mapping of cardiac muscle electro-mechanical dynamics. We will use a specialized high-speed SWIR camera and multi-spectral alternating light sources located outside of the body of label-free mice to test the hypothesis that extracorporeal mapping of spatiotemporal patterns of cardiac activity in vivo is feasible in two optical settings: (1a) wide view objective lens assembly for distanced positioning from the body surface to maximize viewing area and intensity of light illumination and probing. (1b) borescope system in contact with the body surface for imaging with minimal media heterogeneity and light loss. Aim 2: To demonstrate functionality of dual-mode endoscopic SWIR system for label-free mapping electrical excitability and mechanical contractility of cardiac muscle. We will test here the hypothesis that low absorbance and scattering of specific wavelengths in blood permits endoscopic probing of the myocardial electrical and mechanical activation, required for mapping deep muscular tissues in cases of large animals or patients. Accomplishing our aims will open the possibility of a new photonic approach for label- free mapping of dynamic excitation and contractile actions in muscular tissues for in vivo research and clinical applications.

项目概要/摘要 心脏、子宫、膀胱和肠道等器官中肌肉细胞的收缩受以下因素调节： 并正在影响与电激发相关的复杂活动。 研究电气和机械之间的动态作用和耦合的范式转换方法 肌肉组织和器官的特性，以更好地将它们与健康和帕金森病等疾病联系起来 新的短波红外（SWIR；~1-2.5 µm）光成像具有血流量相对较低的波段。 吸收和散射，最近被提议用于深层组织和体内成像。 该项目的总体目标是开发一种体内体外和内窥镜无标记 SWIR 因此，记录了无标签的多光谱数据集。 同时在优化的短波红外波长将提供新的电光谱指纹 我们将使用活体小鼠的跳动心脏作为开发肌肉组织的机械作用的平台。 在多个 SWIR 波长下进行高速探测的技术，以测试体内的一般假设 本征光学成像可以同时表征不同的动态时空模式 我们的具体目标是： 目标 1：证明内在的力量。 多光谱短波红外光成像可用于心肌机电的体外测绘 我们将使用专门的高速短波红外相机和多光谱交替光源。 位于无标记小鼠体外，以检验体外映射的假设 体内心脏活动的时空模式在两种光学设置下是可行的：(1a) 宽视角物镜 用于与身体表面保持一定距离的组件，以最大限度地提高可视面积和光强度 (1b) 与身体表面接触的管道镜系统，以最少的介质进行成像 目标 2：展示双模式内窥镜短波红外系统的功能。 我们将在这里测试心肌的电兴奋性和机械收缩性的无标记映射。 假设血液中特定波长的低吸光度和散射允许内窥镜探测 心肌电和机械激活，在以下情况下绘制深层肌肉组织所需 实现我们的目标将为大型动物或患者开启新的光子方法的可能性。 自由绘制肌肉组织中的动态兴奋和收缩作用，用于体内研究和临床 应用程序。