MOLECULAR CYTOGENETICS

分子细胞遗传学

• 批准号: 8182208
• 负责人: MARGARET A LEVERSHA
• 金额: $ 10.75万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8182208
• 关键词: MOLECULAR; CYTOGENETICS; MOLECULAR; CYTOGENETICS

Molecular Cytogenetics enables the analysis of chromosomal changes over a broad range of focus, from whole genome composition or organization to specific gene copy number or location. It provides a comprehensive genomic context for global or targeted cell biology studies. In contrast to most other approaches, it enables a cell-by-cell survey of chromosomal content, revealing heterogeneity and possible associations within that heterogeneity. Thus cytogenetic analysis remains a simple and efficient first step towards identifying novel areas of genomic change. The Molecular Cytogenetics Core provides MSKCC investigators with effective chromosome-based analyses for human or research animal cells. It processes samples from primary cells, cell lines, or archival tissue, performs chromosome analysis on research samples, using conventional Cytogenetics (chromosome banding and karyotyping) and molecular Cytogenetics procedures based on fluorescence in situ hybridization (FISH), including Spectral Karyotyping (SKY). The Core staff works with investigators to design the most appropriate and efficient analysis for their needs and produces customized probes for specific projects. The Core has assembled a broad range of molecular Cytogenetics resources for human and mouse analysis, including plasmid and BAG clone stocks, as well as chromosome paints. Chromosome analysis is an integral part of research focusing on genomic instability. The Core's experience in karyotyping and chromosome identification provides valuable support to investigators attempting to understand the basis of chromosomal instability in cancer. In addition to specific research applications, the Core also provides an essential function in maintaining Good Laboratory Practice for MSKCC research projects that use cultured cell lines. Karyotype analysis provides basic confirmation and documentation of cell line identity, and is used to monitor chromosomal integrity.

分子细胞遗传学可以分析染色体变化，从广泛的焦点中 整个基因组组成或组织到特定基因拷贝数或位置。它提供了 全球或靶向细胞生物学研究的综合基因组环境。与大多数其他 方法，它可以逐一调查染色体含量，揭示异质性和可能 该异质性内的关联。因此，细胞遗传学分析仍然是一个简单有效的第一步 旨在识别基因组变化的新领域。 分子细胞遗传学核心为MSKCC研究者提供了有效的基于染色体的分析 用于人类或研究动物细胞。它处理来自原代细胞，细胞系或档案组织的样品， 使用常规细胞遗传学（染色体）对研究样品进行染色体分析 基于荧光原位杂交的带和核分型）和分子细胞遗传学程序 （鱼），包括光谱核分型（天空）。核心人员与调查人员合作设计最多 针对他们的需求进行适当有效的分析，并为特定项目生产自定义探针。这 核心已组装了广泛的分子细胞遗传学资源，用于人类和小鼠分析， 包括质粒和袋子克隆汤以及染色体油漆。 染色体分析是关注基因组不稳定性的研究的组成部分。核心的经验 在核分型和染色体识别中，为试图进行的调查人员提供了宝贵的支持 了解癌症中染色体不稳定性的基础。 除了特定的研究应用程序外，核心还提供了维护的重要功能 使用培养细胞系的MSKCC研究项目的良好实验室实践。核型分析 提供了基本的确认和单元线身份的文档，并用于监测染色体 正直。