CORE E

核心E

• 批准号: 7907618
• 负责人: Joanne I Yeh
• 金额: $ 36.42万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7907618
• 关键词: Ammonium Sulfate; Bacteria; Chemicals; Cluster Analysis; Complex; Crystallization; Data; Data Collection; Data Quality; Databases; Diffuse; Diffusion; Doctor of Philosophy; Drug Formulations; Goals; HIV; Individual; Label; Literature; Metals; Methods; Microdialysis; Phase; Polyethylene Glycols; Probability; Procedures; Production; Proteins; Reporting; Resolution; Screening procedure; Signal Transduction; Solubility; Solutions; Structure; System; Techniques; Time; Twin Multiple Birth; Universities; X ray diffraction analysis; X-Ray Diffraction; base; cofactor; genetic regulatory protein; improved; literature survey; meetings; protein complex; small molecule; tool; trend; vapor

Proteins from the Protein Production Core produced in bacteria for crystal structure analysis will routinely be Se-Met labeled to directly enable phasing. For those produced in other hosts or initially provided by collaborators, additional approaches to phasing, such as via multiple isomorphous replacement methods as described below, will be used. The major objectives are (1) to develop crystallization and additive screens formulated specifically for protein complexes to optimize the probability of obtaining diffracting crystals and (2) to solve structures of HIV accessory/regulatory proteins using high-throughput approaches for crystallization, data collection, and structure determination. The ultimate goal is to solve the structures of HIV accessory/regulatory proteins in complexes with cellular proteins and to accomplish this by using rational approaches, as described throughout this proposal.

来自细菌中用于晶体结构分析的蛋白质生产核心的蛋白质将常规 被标记为直接启用相位。对于其他主机或最初提供的人 合作者，其他相位的方法，例如通过多种同构替换方法作为 下面描述的将使用。主要目标是（1）开发结晶和添加屏幕 专门为蛋白质复合物配制，以优化获得衍射晶体的可能性，（2） 使用高通量方法来解决HIV附件/调节蛋白的结构，以进行结晶， 数据收集和结构确定。最终目标是解决艾滋病毒的结构 与细胞蛋白的复合物中的辅助/调节蛋白，并通过使用理性来实现这一目标 在本提案中所述的方法。