Pathology

病理

基本信息

批准号：
7727037
负责人：
Thomas March
金额：
$ 28.71万
依托单位：
LOVELACE BIOMEDICAL & ENVIRONMENTAL RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7727037
关键词：
Acute Alcian Blue Animal Euthanasia Animals Antigens Area Autopsy Biochemistry Blood Blood Cell Count Body Fluids Bronchoalveolar Lavage Bronchoalveolar Lavage Fluid Canis familiaris Cells Chemistry Chronic Clinical Clinical Pathology Data Detection Economic Inflation End Point Evaluation Fibrosis Freezing Hematology Hematoxylin and Eosin Staining Method Histology Histopathology Human Resources Hyperplasia Hypertrophy Immunohistochemistry In Situ Hybridization Laboratories Liquid substance Lung Messenger RNA Methods Molecular Biology Mucous body substance Mustard Gas Organ Paraffin Embedding Pathogenesis Pathologist Pathology Periodic acid Schiff stain method Preparation Procedures Process Proteomics Pulmonary Emphysema Research Institute Research Project Grants Rodent Safety Scientist Serum Services Slide Specimen Staining method Stains Standards of Weights and Measures Therapeutic Intervention Therapeutic Uses Time Tissue Banks Tissue Fixation Tissues Toxic effect Trichrome stain method United States Food and Drug Administration Urinalysis Work experience lung injury morphometry pressure sample collection sample fixation tissue processing

项目摘要

Pathology Core The Pathology Core will be used by all of the projects of the Center. It will coordinate and provide services for specimen collection, clinical hematology and serum chemistry, tissue fixation and processing, preparation of histology specimens, and histopathologic analyses. For Project 5, the core will collect and process tissues and body fluids under standard operating procedures (SOPs) from animals designated for safety assessment of potential therapeutics using Good Laboratory Practices (GLP) as outlined by the FDA. For Projects 2, 3, and 5, pathogenesis of sulfur mustard-induced acute and chronic lung injury will be investigated. Specimens obtained by the core in these projects may be analyzed by clinical pathology and histopathology as well as by methods outlined in Project 1 for identification of potential targets of therapeutic intervention. Specific methods for study will include: 1) routine necropsy procedures for dogs and rodents, 2) full-set tissue collection for histopathologic evaluation of target organ and sytemic toxicity, 3) routine hematology, serum chemistry, and urinalysis for toxicity assessment, 4) bronchoalveolar lavage (BAL) for analysis of cells and fluid, 5) constant pressure inflation-fixation of lungs for stereology/morphometry, 6) hematoxylin and eosin (H&E) staining of paraffin embedded tissues, 7) special stains for mucus (alcian blue and periodic acid-Schiff) and fibrous tissue (Masson's trichrome), 8) morphometry of mucous cell hyperplasia and hypertrophy in the conducting airways, and 9) morphometry for pulmonary emphysema and fibrosis. Additional procedures may include freezing of tissue for biochemistry, molecular biology, and proteomics (Project 1); cryomicrotomy; immunohistochemistry for any antigen in frozen or paraffin-embedded tissue sections;and in situ hybridization for mRNA detection.

病理核心病理核心将由中心的所有项目使用。它将协调并为标本收集，临床血液学和血清化学，组织固定和加工，制备组织学标本和组织病理学分析。对于项目5，核心将收集和处理组织指定用于安全评估的动物的标准操作程序（SOP）下的体液和体液 FDA概述的使用良好实验室实践（GLP）的潜在治疗剂。对于项目2、3，和5，将研究硫芥末引起的急性和慢性肺损伤的发病机理。标本通过临床病理学和组织病理学以及项目1中概述的方法用于识别治疗干预的潜在靶标。具体的研究方法将包括： 1）狗和啮齿动物的常规尸检程序， 2）全设置的组织收集，用于靶器官和肿瘤毒性的组织病理学评估， 3）常规血液学，血清化学和尿液分析用于毒性评估， 4）支气管肺泡灌洗（BAL）用于分析细胞和液体， 5）肺的恒定压力通货膨胀固定用于立体学/形态计量学， 6）石蜡嵌入组织的苏木精和曙红（H＆E）染色， 7）粘液（Alcian蓝色和周期性酸 - chiff）和纤维组织（Masson's Trichrome）的特殊污渍， 8）导电道中的粘液细胞增生和肥大的形态计量学，以及 9）用于肺肺气肿和纤维化的形态计量学。其他程序可能包括冻结用于生物化学，分子生物学和蛋白质组学的组织（项目1）;冷冻切开术；用于冷冻或石蜡包裹组织中任何抗原的免疫组织化学切片；以及原位杂交用于mRNA检测。