ESTABLISHMENT OF SPF RHESUS MACAQUE BREEDING COLONY: HIV THERAPEUTIC AGENT DVMT

SPF级恒河猴繁育群体的建立：HIV治疗剂DVMT

• 批准号: 7961209
• 负责人: KEITH G. MANSFIELD
• 金额: $ 12.85万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7961209
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Animals; Biological; Breeding; Cercopithecine Herpesvirus 1; Computer Retrieval of Information on Scientific Projects Database; Confounding Factors (Epidemiology); Disease; Disease Progression; Funding; Genetic screening method; Goals; Grant; HIV; Health; Housing; Human; Immunity; Infection; Infectious Agent; Institution; Lead; Macaca; Macaca mulatta; Mission; Pathogenesis; Research; Research Personnel; Resources; Role; SIV; Scientist; Simian Retroviruses; Simian T-lymphotropic virus 1; Source; Specific Pathogen Frees; Testing; Therapeutic Agents; United States National Institutes of Health; Universities; Viral; Virus; base; germ free condition; meetings; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The SIV-infected rhesus macaque has emerged as the premiere animal model of human AIDS and has lead to important advances in understanding aspects of disease pathogenesis, the role of viral determinants on disease progression and the impact of host immunity in controlling viral replication. The limited availability of Indian-origin rhesus macaques (Macaca mulatta) specific pathogen free of B virus (BV), Simian T lymphotropic Virus (STLV-1), Simian Retrovirus Type D (SRV-D) and Simian Immunodeficiency Virus (SIV) has become an impediment to investigators conducting animal model based AIDS-related research. Such animals are important for reasons of colony health, and biosafety and to reduce confounding variables associated with co-infection with these viruses. In addition to these requirements, animals free of other infectious agents or of defined MHC type have become increasingly important in the implementation of certain research programs. The NEPRC first established a breeding colony free of BV, STLV-1, SRV-D and SIV in 1988 and this colony has been instrumental in meeting its national resource mission by providing well-defined animals to core and collaborating scientists for use in AIDS-related research. In recognition of the importance of this colony, Harvard University recently funded the construction of a $3.1 M specific pathogen free rhesus macaque breeding facility and a $7.1M SPF macaque housing and testing facility. This application proposes to expand and further refine the existing NEPRC colony to take advantage of these and other improvements. To accomplish this goal we propose the following specific aims: 1) To expand the NEPRC breeding colony specific pathogen free of BV, STLV-1, SRV-D and SIV through internal and external recruitment of well-defined Indian-origin rhesus macaques; 2) To continue virologic testing of the SPF colony and expand microbiologic assessment of the SPF and "super-clean" breeding groups; and 3) To continue MHC typing and genetic testing with the goal of enhancing colony management and the biological resource.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 描述（由申请人提供）：SIV感染的恒河猴已成为人类艾滋病的首屈一指的动物模型，并在理解疾病发病机理方面，病毒决定因素对疾病进展的作用以及宿主免疫在控制病毒复制中的影响方面取得了重要的进步。印度 - 原发性恒河猕猴（Macaca Mulatta）的可用性有限，没有B病毒（BV），Simian T型淋巴机病毒（STLV-1），D型D型（SRV-D）（SRV-D）和Simian替代性病毒（SRV-D）和邻肌免疫缺陷病毒（SIV）对基于调查人员的模型进行了模型的研究。这些动物的重要原因是菌落健康和生物安全的原因，并减少与这些病毒共同感染相关的混杂变量。除这些要求外，没有其他传染性药物或定义的MHC类型的动物在某些研究计划的实施中变得越来越重要。 NEPRC于1988年首次建立了一个没有BV，STLV-1，SRV-D和SIV的繁殖殖民地，该殖民地通过提供明确定义的动物来核心和合作的科学家来实现与艾滋病相关的研究的核心和协作，从而有助于实现其国家资源任务。为了意识到这个殖民地的重要性，哈佛大学最近资助了建造3.1 m的特定病原体猕猴育种设施以及710万美元的SPF猕猴住房和测试设施。该申请建议扩展并进一步完善现有的NEPRC殖民地，以利用这些和其他改进。 为了实现这一目标，我们提出以下特定目的： 1）通过内部和外部招募印度 - 原来的印度原晶状猕猴，扩大了NEPRC繁殖特异性病原体，不具有BV，STLV-1，SRV-D和SIV； 2）继续对SPF菌落的病毒学测试，扩大对SPF和“超级清洁”育种组的微生物学评估； 3）继续MHC打字和基因测试，以增强菌落管理和生物资源。